scholarly journals Determination of Doxycycline Hyclate by Batch and Reverse Flow Injection Analysis Based on the Oxidative Coupling Reaction with 3-Methyl-2-benzothiazolinone Hydrazone hydrochloride (MBTH)

2016 ◽  
Vol 13 (2) ◽  
pp. 489-495
Author(s):  
Baghdad Science Journal
Keyword(s):  
Oxidative Coupling ◽  
Reverse Flow ◽  
Pharmaceutical Preparations ◽  
Coupling Reaction ◽  
Calibration Graph ◽  
Water Soluble ◽  
Green Water ◽  
Doxycycline Hyclate ◽  
Oxidative Coupling Reaction

New, simple and sensitive batch and reverse FIA spectrophotometric methods for the determination of doxycycline hyclate in pure form and in pharmaceutical preparations were proposed. These methods based on oxidative coupling reaction between doxycycline hyclate and 3-methylbenzothiazolinone-2-hydrazone hydrochloride (MBTH) in the presence ammonium ceric sulfate in acidic medium, to form green water-soluble dye that is stable and has a maximum absorbance at 626 nm. A calibration graph shows that a Beer's law is obeyed over the concentration range of 1-80 and 0.5-110 ?g.mL-1 of DCH for the batch and rFIA respectively with detection limit of 0.325 ?g.mL-1 of DCH for r-FIA methods. All different chemicals and physical experimental parameters affecting the development and stability of the colored product were carefully studied. The proposed methods were successfully applied for the determination of DCH in pharmaceutical preparations.

scholarly journals Spectrophotometric Determination of Bisacodyl in Pure and Pharmaceutical Preparation via Oxidative Coupling Organic Reaction

2017 ◽  
Vol 14 (1) ◽  
pp. 181-188
Author(s):  
Baghdad Science Journal
Keyword(s):  
Oxidative Coupling ◽  
Pharmaceutical Preparation ◽  
Pharmaceutical Preparations ◽  
Coupling Reaction ◽  
Molar Absorptivity ◽  
Water Soluble ◽  
Alkaline Media ◽  
Oxidative Coupling Reaction ◽  
Relative Standard ◽  
Limit Of Quantitation

A simple, accurate and sensitive spectrophotometric way is used to determine Bisacodyl in pure and pharmaceutical preparations. The proposed method depends on using 2,4-Dinitrophenylhydrazine as chromogenic reagent . The method was based on the oxidative coupling reaction of Bisacodyl with 2,4-Dinitrophenylhydrazine with Sodium periodate in the presence of sodium hydroxide as alkaline media to form red water soluble dye product , that has a maximum absorption at ?max 522nm . Beer ,s law is obeyed in the concentration of (2.00–20.00) ?g.ml -1 .The molar absorptivity is (6505) L.mol-1.cm-1,a sandall sensitivity of(0.0555) ?g.cm-2), correlation coefficient of (0.9970) , Limitof detection (LOD) (0.0312 ?g.ml-1), limit of Quantitation (LOQ) (0. 3125 ?g.ml-1) and the relative standard deviation of RSD% (1.6). The method gave a successful determination for Bisacodyl in pharmaceutical preparations and the value of recovery % was better than (100.16%)

scholarly journals Spectrophtometric determination of Hydrochlorothiazaide by oxidative coupling with O-Phenylendiamine

2019 ◽  
Vol 24 (2) ◽  
pp. 46
Author(s):  
Rahma Abdul Hameed Hasan1 ◽  
Ali Ibraheem Khaleel2
Keyword(s):  
Oxidative Coupling ◽  
Coupling Reaction ◽  
Molar Absorptivity ◽  
Water Soluble ◽  
Sensitivity Index ◽  
Medium Ph ◽  
Oxidative Coupling Reaction ◽  
Stable Product ◽  
Orange Color

This study includes the development of a new sensitive spectrophotometric method for the determination of a hydrochlorothaizide in aqueous solution and pharmaceultical preparations. The method is based on the oxidative coupling reaction of hydrochlorothaizide with O-phenylendiamine reagent in a acidic medium pH 1.5 in the presence of potassium ferricyanide  to produce an intense orange color, water soluble and stable product , which exhibits maximum absorption at 450 nm. Beer’s law is obeyed over the concentration arange 6 to 48 µg.ml-1 of hydrochlorothaizide, with a molar absorptivity of  3602.17  L.mo1-1 .cm -1 , Sandell’s sensitivity index of 0.03 µg.cm-2 , relative error range not more than 0.215% , and D.L 0.0835µg.ml-1 . The method has been successfully applied for the determination of hydrochlorothaizide in tablets.   http://dx.doi.org/10.25130/tjps.24.2019.030

scholarly journals Spectrophotometric Determination of Phenylephrine Hydrochloride in Pharmaceutical Preparations by Oxidative Coupling Reaction

2020 ◽  
Vol 10 (03) ◽  
pp. 323-327
Author(s):  
Ahmed Hamdi Hattab ◽  
Abdul Majeed Khorsheed Ahmed ◽  
Sunbul M. Anwar
Keyword(s):  
Oxidative Coupling ◽  
Pharmaceutical Preparations ◽  
Coupling Reaction ◽  
Molar Absorptivity ◽  
Simple Condition ◽  
First Line ◽  
Phenylephrine Hydrochloride ◽  
Oxidative Coupling Reaction ◽  
Prevention And Treatment

Phenylephrine is a medication mainly applied as a decongestant to relieve hemorrhoids, increase blood pressure, and dilate the pupil. It is the first-line choice for prevention and treatment of hypotension during spinal anesthesia for cesarean section. However, many methods were used for the determination of this drug and its properties. This paper proposes a method that uses N, N dimethyl-p-phenylenediamine dihydrochloride as a new chromogenic reagent (NNDPH). This method is based on the oxidative coupling reaction of phenylephrine with N, N dimethyl-p-phenylenediamine dihydrochloride with ferric chloride in basic media to form green-blue soluble dye product. The outcomes demonstrate that the maximum absorption is at 680 nm, molar absorptivity of 5.54 × 103 mol-1.cm-1, and Sandell’s sensitivity of 0.038 μg.cm-2. The concentration range of 4 to 22 μg.mL-1 of the product is conformable to Beer’s law. Furthermore, it is applied successfully for estimating the drug properties on a simple condition or pharmaceutical preparations.

scholarly journals SPECTROPHTOTOMETRIC DETERMINATION OF PURE SULFAMETHOXAZOLE IN PHARMACEUTICAL PREPARATIONS BY OXIDATIVE COUPLING REACTION

2021 ◽  
Vol 13 (1) ◽  
pp. 65-76
Author(s):  
Noor M. Ghaib Allah1 ◽  
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Oxidative Coupling ◽  
Sulfonic Acid ◽  
Pharmaceutical Preparations ◽  
Coupling Reaction ◽  
Potassium Iodate ◽  
Oxidative Coupling Reaction ◽  
Sensitive Spectrophotometric Method

A new, simple, rapid and sensitive spectrophotometric method for the determination of sulfamethoxazole in both pure form and pharmaceutical preparations has been reported.The adapted technique based on utilization 4-aminobenzene sulfonic acid as a new modern chromogenic through an oxidative coupling reaction with sulfamethoxazole and potassium iodate in basic media to form orange soluble dye product with absorption maxima at 490 nm. Subject to Beer's law in the range 2–32μg mL-1. The values of molarabsorption coefficient (ε) and correlation coefficient were found to be 9.118 × 103 and0.9999 respectively whereas the Sandels index was 0. 02778 µg.cm-2

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