scholarly journals Internal Standard Corrected Signal Integration Method for Determination of Radioactive Strontium by Online Solid Phase Extraction/ICP-MS

2017 ◽  
Vol 66 (3) ◽  
pp. 181-187 ◽  
Author(s):  
Makoto FURUKAWA ◽  
Makoto MATSUEDA ◽  
Yoshitaka TAKAGAI
Keyword(s):  
Solid Phase Extraction ◽  
Integration Method ◽  
Solid Phase ◽  
Internal Standard ◽  
Phase Extraction ◽  
Signal Integration ◽  
Icp Ms ◽  
Online Solid Phase Extraction

scholarly journals A Modified Method for Determination of Lumefantrine in Human Plasma by HPLC-UV and Combination of Protein Precipitation and Solid-Phase Extraction: Application to a Pharmacokinetic Study

2010 ◽  
Vol 5 ◽  
pp. ACI.S4431 ◽  
Author(s):  
Liusheng Huang ◽  
Patricia S. Lizak ◽  
Anura L. Jayewardene ◽  
Florence Marzan ◽  
Ming-Na Tina Lee ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Human Plasma ◽  
Solid Phase ◽  
Internal Standard ◽  
Pharmacokinetic Interaction ◽  
Gradient Elution ◽  
Protein Precipitation ◽  
Phase Extraction ◽  
Modified Method

An HPLC-UV method was developed and validated for the determination of lumefantrine in human plasma. Lumefantrine and its internal standard halofantrine were extracted from plasma samples using protein precipitation with acetonitrile (0.2% perchloric acid) followed by solid-phase extraction with Hypersep C8 cartridges. Chromatographic separation was performed on a Zorbax SB-CN HPLC column (3.0 × 150 mm, 3.5 μm) with water/methanol (0.1% TFA) as the mobile phases in a gradient elution mode. Detection was performed using UV/vis detector at λ = 335 nm. The method showed to be linear over a range of 50-10,000 ng/mL with acceptable intra- and inter-day precision and accuracy. The mean recoveries were 88.2% for lumefatrine and 84.5% for the I.S. The internal standard halofantrine is readily available from commercial sources. This method was successfully applied to a pharmacokinetic interaction study between a first-line antimalarial combination (artemether—lumefantrine) and antiretroviral therapy.

scholarly journals Determination of Phthalate Plasticisers in Palm Oil Using Online Solid Phase Extraction-Liquid Chromatography (SPE-LC)

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Nazarudin Ibrahim ◽  
Rozita Osman ◽  
Azmui Abdullah ◽  
Norashikin Saim
Keyword(s):  
Solid Phase Extraction ◽  
Palm Oil ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Correlation Coefficients ◽  
Array Detector ◽  
Phase Extraction ◽  
Extraction Solvent ◽  
Online Solid Phase Extraction

Contamination of phthalates plasticisers to food has raised concern as some of the phthalates are suspected to be endocrine disruptors. The phthalates have high affinity with oily environment and analysing these chemicals in such matrices is difficult because of the trace amount of the analyte and interference from matrix. An online solid phase extraction (SPE) technique using a large volume (3.5 mL) injection was developed for the analysis of 6 common plasticisers in palm oil. A simple sample preparation involving alumina as a fat retainer and methanol : acetonitrile (1 : 1) as the extraction solvent was performed prior to the usage of online SPE-LC system. This system consists of two columns, C16for the solid phase extraction (SPE) and C18as the analytical column, and a photo diode array detector. The calibration curves were linear from 5 to 1000 μg L−1, with correlation coefficients above 0.99. The instrumental limit of detection was 3 μg L−1and satisfactory recovery was obtained. A screening on a few samples in the retail market revealed the presence of dibutyl phthalate (DBP) and butylbenzylphthalate (BBP) in the palm oil, with concentration less than 1 mg L−1.

scholarly journals Determination of Trace Elements in High Purity Iron by Solid Phase Extraction Using Bonded Silica/ICP-MS

2003 ◽  
Vol 89 (9) ◽  
pp. 958-961 ◽  
Author(s):  
Shin-ichi HASEGAWA ◽  
Kunikazu IDE ◽  
Takeshi KOBAYASHI ◽  
Koichi SATO ◽  
Shukuro IGARASHI ◽  
...  
Keyword(s):  
Trace Elements ◽  
Solid Phase Extraction ◽  
High Purity ◽  
Solid Phase ◽  
Phase Extraction ◽  
Icp Ms ◽  
Purity Iron ◽  
High Purity Iron

scholarly journals Determination of Coumarin-type Anticoagulants in Human Plasma by HPLC-Electrospray Ionization Tandem Mass Spectrometry with an Ion Trap Detector

2002 ◽  
Vol 48 (1) ◽  
pp. 84-91 ◽  
Author(s):  
Manfred Kollroser ◽  
Caroline Schober
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Solid Phase Extraction ◽  
Electrospray Ionization ◽  
Solid Phase ◽  
Internal Standard ◽  
Tandem Mass ◽  
Phase Extraction ◽  
Ionization Tandem Mass Spectrometry

Abstract Background: Coumarin-type anticoagulants are used for the long-term treatment and prevention of thromboembolic disorders. The identification of these drugs is crucial in patients with an increased prothrombin time of unknown origin. The aim of this study was to develop a sensitive and specific method for the simultaneous determination of phenprocoumon, acenocoumarol, and warfarin in human plasma by HPLC-electrospray ionization tandem mass spectrometry. Methods: After addition of the internal standard, p-chlorowarfarin, plasma samples were extracted using Oasis® MCX solid-phase extraction cartridges. The compounds were separated on a Symmetry C18 column (Waters) with a mobile phase of acetonitrile–1 g/L formic acid (75:25 by volume) at a flow rate of 0.5 mL/min. Results: Extraction and separation of the three drugs and the internal standard were accomplished in 9 min. The overall extraction efficiency was >89% for all three compounds. The limits of detection were 1 μg/L for phenprocoumon and warfarin and 10 μg/L for acenocoumarol. Regression analysis of the calibration data revealed good correlation (r2 ≥0.995) for all compounds. Within-run accuracies for quality-control samples were ± 1% to 7% of the target concentration, with CVs <9%. Conclusions: The proposed method enables the unambiguous identification and quantification of phenprocoumon, warfarin, and acenocoumarol in both clinical and forensic specimens. This method combines a new, rapid solid-phase extraction procedure with an extremely fast chromatographic analysis, which is especially advantageous for clinical laboratories.

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