Puccinia triticina pathotypes-THTT and THTS display complex transcript profiles on Thatcher

Abstract Background: Wheat leaf rust is an important disease worldwide. Understanding the Puccinia triticina (Pt) pathogenic molecular mechanism and inconstant toxic region are highly important for managing the disease. The present study aimed to analyze pathogenic divergences between Pt isolates. Results: Total RNA was extracted from Thatcher infected by two Pt isolates, Tc361_1 (THTT) and Tc284_2 (THTS) at 144 hours post inoculation (hpi). The mRNA then was harvested and sequenced. Results indicated that a total of 2,784 differential expressed genes (DEGs) were detected. Forty-five of genes were specifically expressed in Tc361_1 including transcription initiation factor, transmembrane transporter activity and so on, while 26 of genes were specially expressed in Tc284_2 including GTPase activity, ABC transporter and so on. Sixty-seven differential expressed candidate effectors were screened. qRT-PCR of 14 significantly different expressed genes were carried out to prove that the results of RNA-seq is similar to that of qRT-PCR at 144 hpi and show their expression level in the early stage and the difference between two Pt pathotypes, which revealed the complex transcript profiles. Conclusion: The results obtained in this study might have a solid foundation for the future studies on clarifying the mechanism of pathogenicity differences of Chinese isolates and pathogenic mechanism of Pt.

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Puccinia triticina pathotypes-THTT and THTS display complex transcript profiles on Thatcher

10.21203/rs.2.12168/v2 ◽

2020 ◽

Author(s):

jie Wei ◽

Liping Cui ◽

dongdong Du ◽

na Zhang ◽

qingfang Meng ◽

...

Keyword(s):

Transcription Initiation ◽

Early Stage ◽

Puccinia Triticina ◽

Initiation Factor ◽

Post Inoculation ◽

Qrt Pcr ◽

Transcription Initiation Factor ◽

Differential Expressed Genes ◽

The Difference ◽

Transcript Profiles

Abstract Background: Wheat leaf rust is an important disease worldwide. Understanding the Puccinia triticina f.sp tritici (Pt) pathogenic molecular mechanism and inconstant toxic region are highly important for managing the disease. The present study aimed to analyze pathogenic divergences between Pt isolates. Results: Total RNA was extracted from Thatcher infected by two Pt isolates, Tc361_1 (THTT) and Tc284_2 (THTS) at 144 hours post inoculation (hpi). The mRNA then was harvested and sequenced. Results indicated that a total of 2,784 differential expressed genes (DEGs) were detected. Forty-five genes were specifically expressed in THTT including transcription initiation factor, transmembrane transporter activity and so on, while 26 genes were specially expressed in THTS including GTPase activity, ABC transporter and so on. Fifty-four differential expressed candidate effectors were screened from the tow isolates. Two candidate effectors were chosen and validated on tabacco, and the result showed that they can inhibit the necrosis induced by Bax. qRT-PCR of 12 significantly different expressed genes were carried out to prove that the results of RNA-seq is similar to that of qRT-PCR at 144 hpi and show their expression level in the early stage and the difference between two Pt pathotypes.Conclusion: The results obtained in this study showed that the transcript profiles between THTT or THTS and Thatcher were very complicated.

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Puccinia triticina pathotypes THTT and THTS display complex transcript profiles on wheat cultivar Thatcher

10.21203/rs.2.12168/v5 ◽

2020 ◽

Author(s):

jie Wei ◽

Liping Cui ◽

na Zhang ◽

dongdong Du ◽

qingfang Meng ◽

...

Keyword(s):

Transcription Initiation ◽

Wheat Cultivar ◽

Early Stage ◽

Puccinia Triticina ◽

Differentially Expressed ◽

Post Inoculation ◽

Rna Seq ◽

Initiation Factors ◽

Transcript Profiles ◽

Important Disease

Abstract Background: Wheat leaf rust is an important disease worldwide. Understanding the pathogenic molecular mechanism of Puccinia triticina Eriks . ( Pt ) and the inconstant toxic region is critical for managing the disease. The present study aimed to analyze the pathogenic divergence between Pt isolates. Results: Total RNA was extracted from the wheat cultivar Thatcher infected by two Pt isolates, Tc361_1 (THTT) and Tc284_2 (THTS), at 144 hours post inoculation (hpi). The mRNA was then sequenced, and a total of 2,784 differentially expressed genes (DEGs) were detected. Forty-five genes were specifically expressed in THTT; these genes included transcription initiation factors and genes with transmembrane transporter activity and other genes. Twenty-six genes were specifically expressed in THTS, including genes with GTPase activity, ABC transporters and other genes. Fifty-four differentially expressed candidate effectors were screened from the two isolates. Two candidate effectors were chosen and validated on tobacco, and the results showed that they could inhibit necrosis induced by BAX. qRT-PCR of 12 significant DEGs was carried out to validate that the results are similar to those of RNA-seq at 144 hpi, to show the expression levels of these DEGs in the early stage and to elucidate the differences in expression between the two Pt pathotypes. Conclusion: The results obtained in this study showed that although the two pathotypes of THTT and THTS contribute similar virulence to wheat, there are a large number of genes participate in the interaction with the susceptible wheat cultivar Thatcher, and revealed the pathogenicity of rust is very complicated.

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Puccinia triticina pathotypes- THTT and THTS display complex transcript profiles on wheat cultiver Thatcher

10.21203/rs.2.12168/v3 ◽

2020 ◽

Author(s):

jie Wei ◽

Liping Cui ◽

dongdong Du ◽

na Zhang ◽

qingfang Meng ◽

...

Keyword(s):

Transcription Initiation ◽

Early Stage ◽

Puccinia Triticina ◽

Differentially Expressed ◽

Post Inoculation ◽

Rna Seq ◽

Initiation Factors ◽

Transcript Profiles ◽

Abc Transporter Genes ◽

Important Disease

Abstract Background: Wheat leaf rust is an important disease worldwide. Understanding the Puccinia triticina f. sp. tritici (Pt) pathogenic molecular mechanism and the inconstant toxic region is critical for managing the disease. The present study aimed to analyze pathogenic divergence between Pt isolates.Results: Total RNA was extracted from wheat cultiver Thatcher infected by two Pt isolates, Tc361_1 (THTT) and Tc284_2 (THTS) at 144 hours post inoculation (hpi). The mRNA was then sequenced, and a total of 2,784 differentially expressed genes (DEGs) were detected. Forty-five genes were specifically expressed in THTT; these genes included transcription initiation factors and genes with transmembrane transporter activity and other genes. Twenty-six genes were specifically expressed in THTS, including genes with GTPase activity, ABC transporter genes and other genes. Fifty-four differentially expressed candidate effectors were screened from the two isolates. Two candidate effectors were chosen and validated on tobacco, and the results showed that they could inhibit necrosis induced by Bax. qRT-PCR of 12 significant DEGs was carried out to validate the results are similar to that of RNA-seq at 144 hpi, to show the expression levels of these DEGs in the early stage and to elucidate the differences in expression between the two Pt pathotypes.Conclusion: The results obtained in this study showed that the two pathtypes of THTT and THTS although contribute similar virulence to wheat, there are a large number of genes participate in the interaction with susceptible wheat cultivar Thatcher, and revealed the pathgenicity of rust is very complicated.

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Puccinia triticina pathotypes THTT and THTS display complex transcript profiles on wheat cultivar Thatcher

10.21203/rs.2.12168/v4 ◽

2020 ◽

Author(s):

jie Wei ◽

Liping Cui ◽

na Zhang ◽

dongdong Du ◽

qingfang Meng ◽

...

Keyword(s):

Transcription Initiation ◽

Wheat Cultivar ◽

Early Stage ◽

Puccinia Triticina ◽

Differentially Expressed ◽

Post Inoculation ◽

Rna Seq ◽

Initiation Factors ◽

Transcript Profiles ◽

Important Disease

Abstract Background: Wheat leaf rust is an important disease worldwide. Understanding the pathogenic molecular mechanism of Puccinia triticina Eriks. (Pt) and the inconstant toxic region is critical for managing the disease. The present study aimed to analyze the pathogenic divergence between Pt isolates. Results: Total RNA was extracted from the wheat cultivar Thatcher infected by two Pt isolates, Tc361_1 (THTT) and Tc284_2 (THTS), at 144 hours post inoculation (hpi). The mRNA was then sequenced, and a total of 2,784 differentially expressed genes (DEGs) were detected. Forty-five genes were specifically expressed in THTT; these genes included transcription initiation factors and genes with transmembrane transporter activity and other genes. Twenty-six genes were specifically expressed in THTS, including genes with GTPase activity, ABC transporters and other genes. Fifty-four differentially expressed candidate effectors were screened from the two isolates. Two candidate effectors were chosen and validated on tobacco, and the results showed that they could inhibit necrosis induced by BAX. qRT-PCR of 12 significant DEGs was carried out to validate that the results are similar to those of RNA-seq at 144 hpi, to show the expression levels of these DEGs in the early stage and to elucidate the differences in expression between the two Pt pathotypes. Conclusion: The results obtained in this study showed that although the two pathotypes of THTT and THTS contribute similar virulence to wheat, there are a large number of genes participate in the interaction with the susceptible wheat cultivar Thatcher, and revealed the pathogenicity of rust is very complicated.

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