scholarly journals Complete mitochondrial DNA sequence analysis of Bluespotted seabream, Pagrus caeruleostictus (Perciformes: Sparidae)

2019 ◽  
Author(s):  
Gyamfua Afriyie ◽  
Yusong Guo ◽  
Felix K.A Kuebutornye ◽  
Christian Ayisi Larbi ◽  
Zhongduo Wang

Abstract Background Bluespotted seabream, Pagrus caeruleostictus is a benthopelagic marine species found in tropical regions and a member of the family Sparidae and genus Pagrus . In this article, a mitochondrial DNA sequence by high-throughput technique, Illumina Hiseq, was carried out on muscle of the above species and determined the complete mitogenome. Samples were obtained from species collected from the coast of Ghana, West Africa.Results The complete mitochondrial DNA sequence was 16,653 bases pairs in length (GenBank Accession number: MN319701) and comprises of 37 genes; 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes as well as a control region as in a typical vertebrate mitochondrial DNA gene. The phylogenetic analysis showed P. caeruleostictus clustered with the clade of P. auriga. Conclusion This research will serve as the foundation for molecular genetic studies of Ghanaian fish species. Moreover, it will contribute to the phylogenetics of class Actinopterygii, order Spariformes, family Sparidae and genus Pagrus.

2019 ◽  
Author(s):  
Gyamfua Afriyie ◽  
Yusong Guo ◽  
Felix K.A Kuebutornye ◽  
Christian Ayisi Larbi ◽  
Zhongduo Wang

Abstract Background Bluespotted seabream, Pagrus caeruleostictus is a benthopelagic marine species found in tropical regions and a member of the family Sparidae and genus Pagrus . In this article, a mitochondrial DNA sequence by high-throughput technique, Illumina Hiseq, was carried out on muscle of the above species and determined the complete mitogenome. Samples were obtained from species collected from the coast of Ghana, West Africa.Results The complete mitochondrial DNA sequence was 16,653 bases pairs in length (GenBank Accession number: MN319701) and comprises of 37 genes; 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes as well as a control region as in a typical vertebrate mitochondrial DNA gene. The phylogenetic analysis showed P. caeruleostictus clustered with the clade of P. auriga. Conclusion This research will serve as the foundation for molecular genetic studies of Ghanaian fish species. Moreover, it will contribute to the phylogenetics of class Actinopterygii, order Spariformes, family Sparidae and genus Pagrus.


2020 ◽  
Author(s):  
Gyamfua Afriyie ◽  
Yusong Guo ◽  
Felix K.A Kuebutornye ◽  
Christian Ayisi Larbi ◽  
Zhongduo Wang

Abstract The authors have withdrawn this preprint from Research Square


2021 ◽  
Vol 9 ◽  
Author(s):  
Peng Tian ◽  
Jiaguang Xiao ◽  
Zhiyu Jia ◽  
Feng Guo ◽  
Xiaolei Wang ◽  
...  

Complete mitochondrial DNA sequence data have played a significant role in phylogenetic and evolutionary studies of scleractinian corals. In this study, the complete mitogenome of Psammocora profundacella Gardiner, 1898, collected from Guangdong Province, China, was sequenced by next-generation sequencing for the first time. Psammocora profundacella is the first species for which a mitogenome has been sequenced in the family Psammocoridae. The length of its assembled mitogenome sequence was 16,274 bp, including 13 protein-coding genes, two tRNAs and two rRNAs. Its gene content and gene order were consistent with the other Scleractinia species. All genes were encoded on the H strand and the GC content of the mitochondrial genome was 30.49%. Gene content and order were consistent with the other Scleractinia species. Based on 13 protein-coding genes, Maximum Likelihood phylogenetic analysis showed that P. profundacella belongs to the “Robust” clade. Mitochondrial genome data provide important molecular information for understanding the phylogeny of stony corals. More variable markers and additional species should be sequenced to confirm the evolutionary relationships of Scleractinia in the future.


2020 ◽  
Vol 5 (2) ◽  
pp. 1913-1914
Author(s):  
Gyamfua Afriyie ◽  
Yusong Guo ◽  
Felix K.A Kuebutornye ◽  
Christian Ayisi Larbi ◽  
Zhongduo Wang

2014 ◽  
Vol 27 (1) ◽  
pp. 416-417
Author(s):  
Wei Xu ◽  
Long-Wu Geng ◽  
Min Xu ◽  
Guang-Xiang Tong ◽  
Hai-Feng Jiang

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