Molecular-genetic identification of microorganism strains isolated from activated sludge based on an analysis of nucleotide sequences of the 16s rRNA gene

A molecular-genetic identification of four bacterial strains isolated from activated sludge of urban wastewater treatment plants (Ulan-Ude) and the industrial enterprise OJSC “Selenginsky Pulp and Paper Mill” (Selenginsk) was carried out. Bacterial strains were identified by a capillary sequencer ABI 3130XL Genetic Analyzer (Applied Biosystems) using 16S primers 27F and 1492R at the Genomics Collective Use Center of the Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk. The results were obtained using the method of determination of the direct nucleotide sequence of a 16S rRNA fragment followed by comparison of the nucleotide identity with the sequences deposited in the international database GenBank. Bacterial strains isolated from activated sludge were identified according to the GenBank database: strain B 1.1 corresponds to Paenibacillus dendritiformis strain P411 (similarity 99.93%), strains B 1.2 and B 1.3 correspond to Bacillus licheniformis strain PB399 (similarity 86 and 100%, respectively), strain P 1.1 corresponds to the Paenibacillus polymyxa strain ISSDS-85 (similarity 99.86%). The biochemical properties of the identified strains were determined: amylolytic, proteolytic and lipolytic activity; the ability to ferment carbohydrates in Hiss’ nutrient medium; the ability to form ammonia, urea and nitrate reduction. The bacterial strains isolated from activated sludge may be promising for the destruction of wastewater pollutants. On their basis, it is planned to create a consortium of microorganisms for the destruction of protein and fatty pollutants in wastewater.

Molecular-genetic identification of arbuscular mycorrhiza fungi from Teberda natural reserve

Arbuscular mycorrhiza fungi of soil samples from North Caucasus were identified via Illumina Miseq and universal primers for ITS region. It was shown, that both ITS1 and ITS2 are necessary for identification.

Molecular-genetic identification of chameleon goby Tridentiger trigonocephalus (Gill, 1859) in the Black Sea

The article focuses on the molecular-genetic identification of the chameleon goby, which has recently become established in the Black Sea basin. The natural range of the chameleon goby are the seas of the Far-East. Genetic variation was studied for the16S rRNA mtDNA gene. The obtained genetic data were compared with similar data of chameleon goby from the natural range and other species from the Tridentiger genus. We determined that, according to the 16S rRNA gene, the identification of the goby from the mouth of the Chernaya River as a species of Tridentiger trigonocephalus was confirmed, and the parameters of the variability indicate an extremely low genetic variability for this gene. According to the authors, the adaptive success of the chameleon goby in the conditions of the Black Sea basin is similar to such of invasive species, namely pumpkinseed (Lepomis gibbosus) and stone moroko (Pseudorasbora parva).

Молекулярно-генетическая идентификация токсинообразующих фитопатогенов из родов Fusarium и Penicillium на озимой пшенице

The aim of the study was the molecular-genetic identification (nested-PCR analysis) of pathogens from genera Fusarium and Penicillium, which are dangerous to human and animal health, in the winter wheat. As a result, species from both genera were detected in the winter wheat at different stages of plants development. The most important was the fact that these pathogens were detected in mature seeds. This can present a potential danger of contamination of food raw material with mycotoxins produced by identified fungi. Additionally, the influence of climatic conditions of the year to the spread of tested pathogens was established.

Designing a system of multiplex PCR with hybridization-fluorescent registration of results, on solid substrate, for indication and identification Y. pestis strains

Plague agent strains differ in intraspecific appurtenance, place of origin, as well as their virulence. Development of a rapid and reliable means of Y. pestis strain indication, their identification by intra-specific appurtenance and assessment of their virulence is a relevant issue. To solve the task, we have constructed a system of multiplex PCR with hybridization-fluorescent registration of results on solid substrate, for indication and identification of strains by their belonging to Y. pestis species, sub-species, biovars, phylogenetic lines, also by the presence of the main gene-factors of pathogenicity of chromosomal and plasmid localization. Its effectiveness is confirmed on 114 plague agent strains of various origins. The developed system of multiplex PCR with hybridization-fluorescent registration of results on solid substrate, can be applied for molecular-genetic identification of strains from natural plague foci with specification of their appurtenance to the main phylogenetic branches and assessment of virulence, for enhancement of sanitary-epidemiological control of RF territories and CIS member-states. plague pathogen, strains, indication and identification, PCR