scholarly journals First isolation of Mycobacterium ulcerans from Swabs and Fine-Needle-Aspiration Specimens in Togo.

2019 ◽  
Author(s):  
Tchalare Kondi Makagni ◽  
Maman Issaka ◽  
Piten Ebekalisai ◽  
Disse Kodjo ◽  
Essossimna A. Kadanga ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Slow Growth ◽  
Buruli Ulcer ◽  
Needle Aspiration ◽  
Mycobacterium Ulcerans ◽  
Clinical Samples ◽  
Direct Examination ◽  
Fine Needle ◽  
Pcr Targeting

Abstract Background Buruli ulcer is a skin disease caused by a mycobacterium called Mycobacterium ulcerans . It is prevalent in more than 33 countries on several continents but West Africa is the most affected. The isolation in culture of the bacteria is difficult because of its slow growth and the facilities required. In Togo, studies have been done on the risk factors for Mycobacterium ulcerans infection and the detection of cases by the Ziehl-Neelsen and PCR technique on clinical and environmental samples, but to date no data of isolates from clinical samples are available. The purpose of this study was to perform an in vitro culture of M. ulcerans from swab and fine needle aspiration samples through the confirmation stages of direct examination and IS2404 -PCR. Method A total of 70 clinical samples from Togo and 10 clinical isolates from Benin are analyzed by the three techniques indicated in the diagnosis, in particular the direct examination of acid-fast bacilli (AFB) using the Ziehl-Neelsen staining, the PCR targeting the IS2404 sequence, and the culture after transport of the samples in a transport medium made of Middlebrook 7H9 medium supplemented with a mixture of PANTA and OADC and decontamination by the modified Petroff method. Results The application of the three techniques of diagnosis for clinical samples yielded 44.28% of positivity rates on direct examination of AFB, 35.71% on culture and 77.14% on qPCR IS2404 with a significantly higher rate for qPCR (0.0001). All samples positive for Ziehl-Neelsen staining and culture were also positive for qPCR. Conclusion : Our results show that the culture, despite it difficulty and the slow growth of the bacteria, can be carried out with recommended tools of the mycobacteria culture and a good method of decontamination of the samples can improve the positivity rates. Its realization will allow the assessment of the in vitro sensitivity to the antibiotics used in the treatment and the discovery of new strains of Mycobacterium ulcerans .

Fine Needle Aspiration as a Tool To Establish Primary Human Breast Cancer Cultures in Vitro

1999 ◽  
Vol 43 (6) ◽  
pp. 985-990 ◽  
Author(s):  
Roberto W. Araujo ◽  
Vera Paiva ◽  
Fátima Gartner ◽  
Isabel Amendoeira ◽  
José Martinez Oliveira ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Fine Needle Aspiration ◽  
Human Breast Cancer ◽  
Needle Aspiration ◽  
Human Breast ◽  
Fine Needle ◽  
Primary Human Breast

Optimierung der Schilddrüsen- Feinnadelpunktion

2011 ◽  
Vol 50 (05) ◽  
pp. 189-194 ◽  
Author(s):  
S.-Y. Sheu-Grabellus ◽  
C. Leitzen ◽  
V. Hartung ◽  
K.-W. Schmid ◽  
A. Bockisch ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Confidence Level ◽  
Thyroid Nodules ◽  
Preliminary Test ◽  
Needle Aspiration ◽  
Fine Needle ◽  
Significant Difference ◽  
Thyroid Glands

SummaryObjective: Optimization of a specially developed automatic microaspirator for fine-needle aspiration of suspicious thyroid nodules. Patients, method: In a preliminary test biopsy effectiveness was evaluated in 20 native resected thyroid glands in vitro with both a Cameco® gun and a specially designed microaspirator respectively. In addition in both techniques two different needles (21-G and 27-G) were used to evaluate the influence of these two cannula. Subsequently, 103 thyroid nodules were biopsied in vivo and compared the results with a preliminary series of the same physician. In the workup and evaluation of the cytology the ThinPrep® technology was used. Results: In vitro the automatic microaspirator was superior to Cameco gun in both when using the 21-Gauge and the 27-Gauge needle. In terms of needle sizes a statistically significant difference at the 95% confidence level was evident for both comparisons in favor of 21-gauge needle. In vivo, 91% of punctures with the microaspirator were usable, while in the pre-series only 84% were usable (p > 0.05). Conclusion: The automatic microaspirator is superior to the manual aspiration. Moreover, under sonographic control it is more convenient, to biopsy even very small nodules and lesiosn (down to 4 mm in diameter)

The management of differentiated thyroid cancer in Europe in 1988.

1989 ◽  
Vol 120 (5) ◽  
pp. 547-558 ◽  
Author(s):  
Line Baldet ◽  
Jean-Claude Manderscheid ◽  
Daniel Glinoer ◽  
Claude Jaffiol ◽  
Béatrice Coste-Seignovert ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Case Report ◽  
Total Thyroidectomy ◽  
Fine Needle Aspiration ◽  
Differentiated Thyroid Cancer ◽  
Needle Aspiration ◽  
Suppressive Therapy ◽  
In Vitro Tests ◽  
Fine Needle

Abstract. In order to know how thyroid nodules and differentiated thyroid cancers are investigated and treated in 1988, an international inquiry was performed by mean of a questionnaire based on a well-defined case report of a 35-year-old female with a solitary small thyroid nodule. Clinicians were asked to indicate their diagnostic and therapeutic approaches to the reported case and to some variations. Analysis of the 157 responses from thyroid experts showed that three in vitro tests (sensitive-TSH, free T4 and total T4) and three in vivo tests (99mTc or radioiodide scintiscan, fine needle aspiration and ultrasonography) were performed most frequently. In the case of a solid and cold nodule and in the absence of fine needle aspiration results, 19% of respondents advocated suppressive therapy and 81% surgery. In the same clinical case, but whom fine needle aspiration had been performed and cytology was benign, surgery was advocated by 24%, suppressive therapy by 48% and a regular follow-up without treatment by 28% of respondents. When surgery was performed and the diagnosis was a differentiated thyroid cancer, (near) total thyroidectomy was more frequently chosen than partial thyroidectomy in both papillary (60 and 40%, respectively, of respondents) and follicular (74 and 26%, respectively, of respondents) cancers; 80% of clinicians did not change their surgical technique in relation to histological type of the tumour. Total thyroidectomy was more often recommended in most of the clinical or anatomical variations compared with the basic case report. Pre- or postoperative hormonal therapy was initiated with L-T4 and TSH suppression was controlled by sensitive-TSH and thyroglobulin determinations. After total thyroidectomy, 131I was used with similar modalities for papillary and follicular cancers to ablate a thyroid remnant.

scholarly journals Use of Fine-Needle Aspiration for Diagnosis of Mycobacterium ulcerans Infection

2010 ◽  
Vol 48 (6) ◽  
pp. 2263-2264 ◽  
Author(s):  
V. Cassisa ◽  
A. Chauty ◽  
E. Marion ◽  
M. F. Ardant ◽  
S. Eyangoh ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Mycobacterium Ulcerans ◽  
Fine Needle

scholarly journals Evaluation of the sensitivity to chemotherapeutics or thiazolidinediones of primary anaplastic thyroid cancer cells obtained by fine-needle aspiration

2008 ◽  
Vol 159 (3) ◽  
pp. 283-291 ◽  
Author(s):  
Alessandro Antonelli ◽  
Silvia Martina Ferrari ◽  
Poupak Fallahi ◽  
Piero Berti ◽  
Gabriele Materazzi ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Cell Growth ◽  
Surgical Procedures ◽  
Fine Needle Aspiration ◽  
Anaplastic Thyroid Cancer ◽  
Needle Aspiration ◽  
Chemotherapeutic Agents ◽  
Inhibition Of Proliferation ◽  
Fine Needle

ObjectiveAnaplastic thyroid cancer (ATC) is often unoperable and chemotherapy and radiotherapy are the main treatments. Until now ‘primary ATC cell cultures’ (ANA) have been developed from surgical biopsies. The possibility to obtain ANA from fine-needle aspiration (FNA-ANA) and to test their sensitivity to different drugs could increase the effectiveness of treatments and avoid unnecessary surgical procedures.DesignTo obtain FNA-ANA from six ATC patients before undergoing surgery and to evaluate the chemosensitivity of FNA-ANA to chemotherapeutic agents and thiazolidinediones (TZD).Methods and resultsFNA-ANA from the six ATC patients were cultured in RPMI 1640 and propagated in DMEM. Chemosensitivity was evaluated by inhibiting the proliferation with increasing concentrations of five different chemotherapeutic agents (bleomycin, cisplatin, gemcitabine, etoposide, and carboplatin) or TZD (rosiglitazone). Chemotherapeutic agents significantly inhibited (P<0.0001) FNA-ANA proliferation, such as TZD (P<0.001); etoposide was the most effective in reducing cell growth. Another ANA culture for each patient was obtained from a biopsy specimen; the results for the chemosensitivity tests were similar to those obtained with FNA-ANA. The V600EBRAF mutation was observed in two ATC patients; the inhibition of proliferation by drugs was similar in tumors with or without V600EBRAF mutation.ConclusionsOur study demonstrates 1) the possibility to obtain FNA-ANA, and opens the way to the use of FNA-ANA to test the chemosensitivity to different drugs (chemotherapeutic agents or TZD; and possibly the radiosensitivity) in each patient, avoiding unnecessary surgical procedures and the administration of inactive chemotherapeutics; and 2) that etoposide is highly effective in reducing ATC cell growth in vitro.

scholarly journals Efficiency of Fine-Needle Aspiration Compared with Other Sampling Techniques for Laboratory Diagnosis of Buruli Ulcer Disease

2010 ◽  
Vol 48 (10) ◽  
pp. 3732-3734 ◽  
Author(s):  
K.-H. Herbinger ◽  
M. Beissner ◽  
K. Huber ◽  
N.-Y. Awua-Boateng ◽  
J. Nitschke ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Buruli Ulcer ◽  
Laboratory Diagnosis ◽  
Needle Aspiration ◽  
Sampling Techniques ◽  
Fine Needle ◽  
Ulcer Disease
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