Adenovirus Virus Expressing Myostatin- Somatostatin Fusing Gene Promote the Growth Rate of the Mice

Abstract Myostatin (MSTN) and somatostatin (SST) are negative regulate factors in the animals. Immunized animals with these proteins induce the related immune response, which could neutralize the normal inhibitory effects of these proteins. Since adenovirus was the mostly wildly used vector for delivering vaccine antigen, here, a recombinant adenovirus expressing MSTN-SST were constructed and mice were inoculated with the recombinant adenovirus. As our predicted, immunization with this adenovirus one time or two times were all induced the specific immune response and resulting in an improve growth rate and muscle mass. And boost immunization improved the effect of the immunization. Accordingly, adenovirus can be used as a vector for deliver myostatin and somatostatin gene, so as to increase animal growth rate and muscle mass of the animals.

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Adenovirus Virus Expressing Myostatin- Somatostatin Fusing Gene Promote the Growth Rate of the Mice

10.21203/rs.2.19059/v2 ◽

2020 ◽

Author(s):

Xinglong Wang ◽

Gaoxiao Xu ◽

Ning Zhang ◽

Xingfa Chen ◽

Dongling Yu

Keyword(s):

Immune Response ◽

Growth Rate ◽

Antibody Response ◽

Muscle Mass ◽

Recombinant Adenovirus ◽

Pathological Change ◽

Inhibitory Effects ◽

Booster Immunization ◽

Related Cell ◽

Animal Growth

Abstract Background Myostatin (MSTN) and somatostatin (SST) are all involved in regulation the growth of the animals. Animals immunized with these proteins induce the related immune response, which could neutralize the normal inhibitory effects of these two proteins. Here, a recombinant adenovirus expressing MSTN-SST was constructed and used to deliver these two antigen to mice, so as to check the immunize efficacy of these proteins delivered in this way. Results The adenovirus expression fusing protein MSTN-SST was successfully rescued on HEK293A cell as adenovirus related cell pathological change were observed after transfection with the adenovirus genome harboring MSTN-SST gene. The expression of MSTN-SST were confirmed using western blotting and indirect immunofluorescence. Immunization with the recombinant adenovirus once or twice were successfully induce immune response against MSTN-SST and resulted in an improved growth rate and muscle mass. In addition, a booster immunization leaded a better results shown as higher antibody response and mice growth rate. Conclusions Accordingly, adenovirus can be used as a vector for deliver myostatin and somatostatin gene to increase animal growth rate and muscle mass .

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Adenovirus Expressing the Myostatin-Somatostatin-fusing Gene Increases Mouse Growth Rate

10.21203/rs.2.19059/v3 ◽

2020 ◽

Author(s):

Gaoxiao Xu ◽

Ning Zhang ◽

Siying Liu ◽

Tingting Wei ◽

Xingfa Chen ◽

...

Keyword(s):

Growth Rate ◽

Growth Performance ◽

Muscle Mass ◽

Transforming Growth Factor ◽

Recombinant Adenovirus ◽

Muscle Growth ◽

Active Immunisation ◽

Gh Secretion ◽

Booster Immunisation ◽

Animal Growth

Abstract BackgroundMyostatin (MSTN), a type of transforming growth factor, can negatively regulate skeletal muscle growth. Immunisation of mice with recombinant yeast expressing MSTN increased bodyweight and muscle composition of them. Somatostatin (SST) is an inhibitory effector of growth hormone (GH), and active immunisation against SST with a DNA vaccine improves the growth performance of piglets via an influence on GH secretion. Here, a recombinant adenovirus was constructed and used to deliver MSTN-SST to mice to achieve the goal of regulation the growth performance of the mice. ResultsA recombinant adenovirus, rAd-MSTN-SST, expressing the MSTN-SST fusing protein was successfully rescued in HEK293A cells. The expressions of MSTN-SST were confirmed by western blotting and indirect immunofluorescence. Mice immunised with rAd-MSTN-SST were successfully induced to have an immune response against MSTN-SST, which increased their growth rate and muscle mass. In addition, a booster immunisation was beneficial in terms of higher antibody response and mouse growth rate. ConclusionsFusing protein MSTN-SST is a good candidate for regulating animal growth rate and muscle mass. Adenovirus can be used as a vector for delivering MSTN and SST to animals.

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Adenovirus Expressing the Myostatin-Somatostatin-fusing Gene Increases Mouse Growth Rate

10.21203/rs.2.19059/v4 ◽

2020 ◽

Author(s):

Gaoxiao Xu ◽

Ning Zhang ◽

Siying Liu ◽

Tingting Wei ◽

Xingfa Chen ◽

...

Keyword(s):

Growth Rate ◽

Growth Performance ◽

Muscle Mass ◽

Transforming Growth Factor ◽

Recombinant Adenovirus ◽

Muscle Growth ◽

Active Immunisation ◽

Gh Secretion ◽

Booster Immunisation ◽

Animal Growth

Abstract Background Myostatin (MSTN), a type of transforming growth factor, can negatively regulate skeletal muscle growth. Immunisation of mice with recombinant yeast expressing MSTN increased bodyweight and muscle composition of them. Somatostatin (SST) is an inhibitory effector of growth hormone (GH), and active immunisation against SST with a DNA vaccine improves the growth performance of piglets via an influence on GH secretion. Here, a recombinant adenovirus was constructed and used to deliver MSTN-SST to mice to achieve the goal of regulation the growth performance of the mice. Results A recombinant adenovirus, rAd-MSTN-SST, expressing the MSTN-SST fusing protein was successfully rescued in HEK293A cells. The expressions of MSTN-SST were confirmed by western blotting and indirect immunofluorescence. Mice immunised with rAd-MSTN-SST were successfully induced to have an immune response against MSTN-SST, which increased their growth rate and muscle mass. In addition, a booster immunisation was beneficial in terms of higher antibody response and mouse growth rate. Conclusions Fusing protein MSTN-SST is a good candidate for regulating animal growth rate and muscle mass. Adenovirus can be used as a vector for delivering MSTN and SST to animals.

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Adenovirus Expressing the Myostatin-Somatostatin-fusing Gene Increases Mouse Growth Rate

10.21203/rs.2.19059/v5 ◽

2020 ◽

Author(s):

Gaoxiao Xu ◽

Ning Zhang ◽

Siying Liu ◽

Tingting Wei ◽

Xingfa Chen ◽

...

Keyword(s):

Growth Rate ◽

Growth Performance ◽

Muscle Mass ◽

Transforming Growth Factor ◽

Recombinant Adenovirus ◽

Muscle Growth ◽

Active Immunisation ◽

Gh Secretion ◽

Booster Immunisation ◽

Animal Growth

Abstract Background Myostatin (MSTN), a type of transforming growth factor, can negatively regulate skeletal muscle growth. Immunisation of mice with recombinant yeast expressing MSTN increased bodyweight and muscle composition of them. Somatostatin (SST) is an inhibitory effector of growth hormone (GH), and active immunisation against SST with a DNA vaccine improves the growth performance of piglets via an influence on GH secretion. Here, a recombinant adenovirus was constructed and used to deliver MSTN-SST to mice to achieve the goal of regulation the growth performance of the mice. Results A recombinant adenovirus, rAd-MSTN-SST, expressing the MSTN-SST fusing protein was successfully rescued in HEK293A cells. The expressions of MSTN-SST were confirmed by western blotting and indirect immunofluorescence. Mice immunised with rAd-MSTN-SST were successfully induced to have an immune response against MSTN-SST, which increased their growth rate and muscle mass. In addition, a booster immunisation was beneficial in terms of higher antibody response and mouse growth rate. Conclusions Fusing protein MSTN-SST is a good candidate for regulating animal growth rate and muscle mass. Adenovirus can be used as a vector for delivering MSTN and SST to animals.

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Adenovirus Expressing the Myostatin-Somatostatin-fusing Gene Increases Mouse Growth Rate

10.21203/rs.2.19059/v6 ◽

2020 ◽

Author(s):

Gaoxiao Xu ◽

Ning Zhang ◽

Siying Liu ◽

Tingting Wei ◽

Xingfa Chen ◽

...

Keyword(s):

Growth Rate ◽

Growth Performance ◽

Muscle Mass ◽

Transforming Growth Factor ◽

Recombinant Adenovirus ◽

Muscle Growth ◽

Active Immunisation ◽

Gh Secretion ◽

Booster Immunisation ◽

Animal Growth

Abstract Background Myostatin (MSTN), a type of transforming growth factor, can negatively regulate skeletal muscle growth. Immunisation of mice with recombinant yeast expressing MSTN increased bodyweight and muscle composition of them. Somatostatin (SST) is an inhibitory effector of growth hormone (GH), and active immunisation against SST with a DNA vaccine improves the growth performance of piglets via an influence on GH secretion. Here, a recombinant adenovirus was constructed and used to deliver MSTN-SST to mice to achieve the goal of regulation the growth performance of the mice. Results A recombinant adenovirus, rAd-MSTN-SST, expressing the MSTN-SST fusing protein was successfully rescued in HEK293A cells. The expressions of MSTN-SST were confirmed by western blotting and indirect immunofluorescence. Mice immunised with rAd-MSTN-SST were successfully induced to have an immune response against MSTN-SST, which increased their growth rate and muscle mass. In addition, a booster immunisation was beneficial in terms of higher antibody response and mouse growth rate. Conclusions Fusing protein MSTN-SST is a good candidate for regulating animal growth rate and muscle mass. Adenovirus can be used as a vector for delivering MSTN and SST to animals.

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