scholarly journals Adenovirus Virus Expressing Myostatin- Somatostatin Fusing Gene Promote the Growth Rate of the Mice

2020 ◽  
Author(s):  
Xinglong Wang ◽  
Gaoxiao Xu ◽  
Ning Zhang ◽  
Xingfa Chen ◽  
Dongling Yu
Keyword(s):  
Immune Response ◽  
Growth Rate ◽  
Antibody Response ◽  
Muscle Mass ◽  
Recombinant Adenovirus ◽  
Pathological Change ◽  
Inhibitory Effects ◽  
Booster Immunization ◽  
Related Cell ◽  
Animal Growth

Abstract Background Myostatin (MSTN) and somatostatin (SST) are all involved in regulation the growth of the animals. Animals immunized with these proteins induce the related immune response, which could neutralize the normal inhibitory effects of these two proteins. Here, a recombinant adenovirus expressing MSTN-SST was constructed and used to deliver these two antigen to mice, so as to check the immunize efficacy of these proteins delivered in this way. Results The adenovirus expression fusing protein MSTN-SST was successfully rescued on HEK293A cell as adenovirus related cell pathological change were observed after transfection with the adenovirus genome harboring MSTN-SST gene. The expression of MSTN-SST were confirmed using western blotting and indirect immunofluorescence. Immunization with the recombinant adenovirus once or twice were successfully induce immune response against MSTN-SST and resulted in an improved growth rate and muscle mass. In addition, a booster immunization leaded a better results shown as higher antibody response and mice growth rate. Conclusions Accordingly, adenovirus can be used as a vector for deliver myostatin and somatostatin gene to increase animal growth rate and muscle mass .

scholarly journals Adenovirus Virus Expressing Myostatin- Somatostatin Fusing Gene Promote the Growth Rate of the Mice

2019 ◽  
Author(s):  
Xinglong Wang ◽  
Gaoxiao Xu ◽  
Ning Zhang ◽  
Xingfa Chen ◽  
Dongling Yu
Keyword(s):  
Immune Response ◽  
Growth Rate ◽  
Muscle Mass ◽  
Recombinant Adenovirus ◽  
Specific Immune Response ◽  
Vaccine Antigen ◽  
Inhibitory Effects ◽  
Boost Immunization ◽  
Animal Growth

Abstract Myostatin (MSTN) and somatostatin (SST) are negative regulate factors in the animals. Immunized animals with these proteins induce the related immune response, which could neutralize the normal inhibitory effects of these proteins. Since adenovirus was the mostly wildly used vector for delivering vaccine antigen, here, a recombinant adenovirus expressing MSTN-SST were constructed and mice were inoculated with the recombinant adenovirus. As our predicted, immunization with this adenovirus one time or two times were all induced the specific immune response and resulting in an improve growth rate and muscle mass. And boost immunization improved the effect of the immunization. Accordingly, adenovirus can be used as a vector for deliver myostatin and somatostatin gene, so as to increase animal growth rate and muscle mass of the animals.

scholarly journals Adenovirus Expressing the Myostatin-Somatostatin-fusing Gene Increases Mouse Growth Rate

2020 ◽  
Author(s):  
Gaoxiao Xu ◽  
Ning Zhang ◽  
Siying Liu ◽  
Tingting Wei ◽  
Xingfa Chen ◽  
...  
Keyword(s):  
Growth Rate ◽  
Growth Performance ◽  
Muscle Mass ◽  
Transforming Growth Factor ◽  
Recombinant Adenovirus ◽  
Muscle Growth ◽  
Active Immunisation ◽  
Gh Secretion ◽  
Booster Immunisation ◽  
Animal Growth

Abstract BackgroundMyostatin (MSTN), a type of transforming growth factor, can negatively regulate skeletal muscle growth. Immunisation of mice with recombinant yeast expressing MSTN increased bodyweight and muscle composition of them. Somatostatin (SST) is an inhibitory effector of growth hormone (GH), and active immunisation against SST with a DNA vaccine improves the growth performance of piglets via an influence on GH secretion. Here, a recombinant adenovirus was constructed and used to deliver MSTN-SST to mice to achieve the goal of regulation the growth performance of the mice. ResultsA recombinant adenovirus, rAd-MSTN-SST, expressing the MSTN-SST fusing protein was successfully rescued in HEK293A cells. The expressions of MSTN-SST were confirmed by western blotting and indirect immunofluorescence. Mice immunised with rAd-MSTN-SST were successfully induced to have an immune response against MSTN-SST, which increased their growth rate and muscle mass. In addition, a booster immunisation was beneficial in terms of higher antibody response and mouse growth rate. ConclusionsFusing protein MSTN-SST is a good candidate for regulating animal growth rate and muscle mass. Adenovirus can be used as a vector for delivering MSTN and SST to animals.

scholarly journals Adenovirus Expressing the Myostatin-Somatostatin-fusing Gene Increases Mouse Growth Rate

2020 ◽  
Author(s):  
Gaoxiao Xu ◽  
Ning Zhang ◽  
Siying Liu ◽  
Tingting Wei ◽  
Xingfa Chen ◽  
...  
Keyword(s):  
Growth Rate ◽  
Growth Performance ◽  
Muscle Mass ◽  
Transforming Growth Factor ◽  
Recombinant Adenovirus ◽  
Muscle Growth ◽  
Active Immunisation ◽  
Gh Secretion ◽  
Booster Immunisation ◽  
Animal Growth

Abstract Background Myostatin (MSTN), a type of transforming growth factor, can negatively regulate skeletal muscle growth. Immunisation of mice with recombinant yeast expressing MSTN increased bodyweight and muscle composition of them. Somatostatin (SST) is an inhibitory effector of growth hormone (GH), and active immunisation against SST with a DNA vaccine improves the growth performance of piglets via an influence on GH secretion. Here, a recombinant adenovirus was constructed and used to deliver MSTN-SST to mice to achieve the goal of regulation the growth performance of the mice. Results A recombinant adenovirus, rAd-MSTN-SST, expressing the MSTN-SST fusing protein was successfully rescued in HEK293A cells. The expressions of MSTN-SST were confirmed by western blotting and indirect immunofluorescence. Mice immunised with rAd-MSTN-SST were successfully induced to have an immune response against MSTN-SST, which increased their growth rate and muscle mass. In addition, a booster immunisation was beneficial in terms of higher antibody response and mouse growth rate. Conclusions Fusing protein MSTN-SST is a good candidate for regulating animal growth rate and muscle mass. Adenovirus can be used as a vector for delivering MSTN and SST to animals.

scholarly journals Adenovirus Expressing the Myostatin-Somatostatin-fusing Gene Increases Mouse Growth Rate

2020 ◽  
Author(s):  
Gaoxiao Xu ◽  
Ning Zhang ◽  
Siying Liu ◽  
Tingting Wei ◽  
Xingfa Chen ◽  
...  
Keyword(s):  
Growth Rate ◽  
Growth Performance ◽  
Muscle Mass ◽  
Transforming Growth Factor ◽  
Recombinant Adenovirus ◽  
Muscle Growth ◽  
Active Immunisation ◽  
Gh Secretion ◽  
Booster Immunisation ◽  
Animal Growth

Abstract Background Myostatin (MSTN), a type of transforming growth factor, can negatively regulate skeletal muscle growth. Immunisation of mice with recombinant yeast expressing MSTN increased bodyweight and muscle composition of them. Somatostatin (SST) is an inhibitory effector of growth hormone (GH), and active immunisation against SST with a DNA vaccine improves the growth performance of piglets via an influence on GH secretion. Here, a recombinant adenovirus was constructed and used to deliver MSTN-SST to mice to achieve the goal of regulation the growth performance of the mice. Results A recombinant adenovirus, rAd-MSTN-SST, expressing the MSTN-SST fusing protein was successfully rescued in HEK293A cells. The expressions of MSTN-SST were confirmed by western blotting and indirect immunofluorescence. Mice immunised with rAd-MSTN-SST were successfully induced to have an immune response against MSTN-SST, which increased their growth rate and muscle mass. In addition, a booster immunisation was beneficial in terms of higher antibody response and mouse growth rate. Conclusions Fusing protein MSTN-SST is a good candidate for regulating animal growth rate and muscle mass. Adenovirus can be used as a vector for delivering MSTN and SST to animals.

scholarly journals Adenovirus Expressing the Myostatin-Somatostatin-fusing Gene Increases Mouse Growth Rate

2020 ◽  
Author(s):  
Gaoxiao Xu ◽  
Ning Zhang ◽  
Siying Liu ◽  
Tingting Wei ◽  
Xingfa Chen ◽  
...  
Keyword(s):  
Growth Rate ◽  
Growth Performance ◽  
Muscle Mass ◽  
Transforming Growth Factor ◽  
Recombinant Adenovirus ◽  
Muscle Growth ◽  
Active Immunisation ◽  
Gh Secretion ◽  
Booster Immunisation ◽  
Animal Growth

Abstract Background Myostatin (MSTN), a type of transforming growth factor, can negatively regulate skeletal muscle growth. Immunisation of mice with recombinant yeast expressing MSTN increased bodyweight and muscle composition of them. Somatostatin (SST) is an inhibitory effector of growth hormone (GH), and active immunisation against SST with a DNA vaccine improves the growth performance of piglets via an influence on GH secretion. Here, a recombinant adenovirus was constructed and used to deliver MSTN-SST to mice to achieve the goal of regulation the growth performance of the mice. Results A recombinant adenovirus, rAd-MSTN-SST, expressing the MSTN-SST fusing protein was successfully rescued in HEK293A cells. The expressions of MSTN-SST were confirmed by western blotting and indirect immunofluorescence. Mice immunised with rAd-MSTN-SST were successfully induced to have an immune response against MSTN-SST, which increased their growth rate and muscle mass. In addition, a booster immunisation was beneficial in terms of higher antibody response and mouse growth rate. Conclusions Fusing protein MSTN-SST is a good candidate for regulating animal growth rate and muscle mass. Adenovirus can be used as a vector for delivering MSTN and SST to animals.

The nature of the immune response of the mouse to the bile duct cestode, Hymenolepis microstoma

Parasitology ◽  
1971 ◽  
Vol 62 (2) ◽  
pp. 285-294 ◽  
Author(s):  
G. D. Moss
Keyword(s):  
Immune Response ◽  
Growth Rate ◽  
Bile Duct ◽  
Antibody Response ◽  
Worm Burden ◽  
Technical Assistance ◽  
Immune Reaction ◽  
Slowing Down ◽  
Cellular Immune ◽  
Hymenolepis Microstoma

The immune reaction of the mouse to the cestode, Hymenolepis microstoma, has been investigated. The mouse can carry a worm burden of up to 500 mg wet wt and there is no evidence either of rejection of the parasite by the mouse or a slowing down of growth rate of the mouse as a result of the parasite.The nature of the antibody response has been investigated with regard to the type of antibody, onset of production, and titre.The nature of the antibody is discussed together with the fact that there is no rejection of the parasite. The possibility that a cellular immune response might be necessary for rejection is also discussed.I should like to express my thanks to Dr T. S. C. Orr of Fisons Research Laboratories, Loughborough, for many helpful comments and suggestions during the course of this work.My thanks are due also to Professor C. A. Hopkins who put the facilities of the Wellcome Laboratory at my disposal at all times. My thanks, too, to Miss Gillian Moore whose technical assistance was invaluable.The work was in part supported by a Grant from Fisons Ltd.

Gene therapy with recombinant adenovirus vectors: evaluation of the host immune response

1997 ◽  
Vol 57 (1-3) ◽  
pp. 19-25 ◽  
Author(s):  
Marielle Christ ◽  
Monika Lusky ◽  
Fabienne Stoeckel ◽  
Dominique Dreyer ◽  
Annick Dieterlé ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Immune Response ◽  
Recombinant Adenovirus ◽  
Host Immune Response ◽  
Adenovirus Vectors

scholarly journals Booster immunization with a fractional dose of Prevnar 13 affects cell-mediated immune response but not humoral immunity in CD-1 mice

Heliyon ◽  
2021 ◽  
pp. e07314
Author(s):  
Rose-Marie Catalioto ◽  
Claudio Valenti ◽  
Francesca Bellucci ◽  
Cecilia Cialdai ◽  
Maria Altamura ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immunity ◽  
Booster Immunization ◽  
Cell Mediated Immune Response ◽  
Fractional Dose

scholarly journals Immunological imprinting of the antibody response in COVID-19 patients

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Teresa Aydillo ◽  
Alexander Rombauts ◽  
Daniel Stadlbauer ◽  
Sadaf Aslam ◽  
Gabriela Abelenda-Alonso ◽  
...  
Keyword(s):  
Immune Response ◽  
Severe Acute Respiratory Syndrome ◽  
Antibody Response ◽  
Humoral Immune Response ◽  
Nucleocapsid Protein ◽  
Spike Protein ◽  
Ongoing Research ◽  
Variable Regions ◽  
Humoral Immune ◽  
Human Coronaviruses

AbstractIn addition to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), humans are also susceptible to six other coronaviruses, for which consecutive exposures to antigenically related and divergent seasonal coronaviruses are frequent. Despite the prevalence of COVID-19 pandemic and ongoing research, the nature of the antibody response against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is unclear. Here we longitudinally profile the early humoral immune response against SARS-CoV-2 in hospitalized coronavirus disease 2019 (COVID-19) patients and quantify levels of pre-existing immunity to OC43, HKU1 and 229E seasonal coronaviruses, and find a strong back-boosting effect to conserved but not variable regions of OC43 and HKU1 betacoronaviruses spike protein. However, such antibody memory boost to human coronaviruses negatively correlates with the induction of IgG and IgM against SARS-CoV-2 spike and nucleocapsid protein. Our findings thus provide evidence of immunological imprinting by previous seasonal coronavirus infections that can potentially modulate the antibody profile to SARS-CoV-2 infection.

scholarly journals Antibody response to immunization with influenza A/USSR/77 (H1N1) virus in young individuals primed or unprimed for A/New Jersey/76 (H1N1) virus

1981 ◽  
Vol 87 (2) ◽  
pp. 201-209 ◽  
Author(s):  
N. Masurel ◽  
P. Ophof ◽  
P. de Jong
Keyword(s):  
New Jersey ◽  
Side Effects ◽  
Antibody Response ◽  
Virus Vaccine ◽  
Influenza A ◽  
H1n1 Virus ◽  
Vaccine Dose ◽  
Booster Vaccination ◽  
Booster Immunization ◽  
Group A

SummaryA group of 269 pupils of the Harbour and Transport Training Institute in Rotterdam (group A), aged 13–20 years, and of 109 patients of the Dr Mr Willem van den Bergh Foundation at Noordwijk (group B), aged 11–21 years, were immunized with a whole virus vaccine containing 10, 20, or 40 μg HA of A/USSR/92/77 (H1N1) influenza virus. A booster vaccination was administered 6 weeks later with 20 μg HA of the same virus. Many of the participants had been immunized during the two preceding years with a whole virus vaccine containing A/New Jersey/8/76 (H1N1) (A/NJ/76) virus. The side-effects, mostly of a moderate nature, increased with the dose of virus in the vaccine. In group A side effects were least frequent in the vaccinees who had never received A/NJ/76 vaccine. A single dose of A/USSR/77 vaccine did not produce satisfactory levels of homologous antibodies. After booster immunization with 20 μg HA of A/USSR/77 virus participants showed a higher homologous antibody response in all vaccine-dose groups if they had not been immunized with A/NJ/76 virus in previous years. After primary and especially after booster immunization with A/USSR/77 virus, a very high response against A/NJ/76 virus and adequate levels of A/NJ/76 antibody were found in participants who had been immunized previously with A/NJ/76 virus. Those who had not been immunized with this virus previously showed no or a very low antibody response to A/NJ/76 virus.

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