No gender difference in synaptic potentiation in the anterior cingulate cortex of adult mice

2020 ◽  
Author(s):  
Ren-Hao Liu ◽  
Man Xue ◽  
Xu-Hui Li ◽  
Min Zhuo
Keyword(s):  
Synaptic Plasticity ◽  
Gender Difference ◽  
Anterior Cingulate Cortex ◽  
Emotional Responses ◽  
Cingulate Cortex ◽  
Anterior Cingulate ◽  
Male Mice ◽  
Adult Mice ◽  
Significant Difference

Abstract Gender differences in certain types of pain sensitivity and emotional responses have been previously reported. Synaptic plasticity is a key cellular mechanism for pain perception and emotional regulation, including long-term potentiation (LTP) and long-term depression (LTD). However, it is unclear whether there is a gender difference at synaptic level. Recent studies indicate that excitatory transmission and plasticity in the anterior cingulate cortex (ACC) are critical in chronic pain and pain related emotional responses. In the present study, we used 64-channel multielectrode (MED64) system to record synaptic plasticity in the ACC of male and female adult mice. We found that there was no significant difference in theta-burst stimulation (TBS)-induced LTP between female and male mice. Furthermore, the recruitment of inactive channels was also not different. For LTD, we found that LTD was greater in slices of ACC in male mice than female mice. Our results demonstrate that LTP in the ACC does not show any gender-related difference.

scholarly journals Sex difference in synaptic plasticity in the anterior cingulate cortex of adult mice

2020 ◽  
Author(s):  
Ren-Hao Liu ◽  
Man Xue ◽  
Xu-Hui Li ◽  
Min Zhuo
Keyword(s):  
Synaptic Plasticity ◽  
Anterior Cingulate Cortex ◽  
Sex Difference ◽  
Emotional Responses ◽  
Cingulate Cortex ◽  
Anterior Cingulate ◽  
Male Mice ◽  
Adult Mice ◽  
Significant Difference

Abstract Sex differences in certain types of pain sensitivity and emotional responses have been previously reported. Synaptic plasticity is a key cellular mechanism for pain perception and emotional regulation, including long-term potentiation (LTP) and long-term depression (LTD). However, it is unclear whether there is a sex difference at synaptic level. Recent studies indicate that excitatory transmission and plasticity in the anterior cingulate cortex (ACC) are critical in chronic pain and pain related emotional responses. In the present study, we used 64-channel multielectrode (MED64) system to record synaptic plasticity in the ACC of male and female adult mice. We found that there was no significant difference in theta-burst stimulation (TBS)-induced LTP between female and male mice. Furthermore, the recruitment of inactive channels was also not different. For LTD, we found that LTD was greater in slices of ACC in male mice than female mice. Our results demonstrate that LTP in the ACC does not show any sex-related difference.

scholarly journals NMDA GluN2C/2D receptors contribute to synaptic regulation and plasticity in the anterior cingulate cortex of adult mice

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Qi-Yu Chen ◽  
Xu-Hui Li ◽  
Jing-Shan Lu ◽  
Yinglu Liu ◽  
Jung-Hyun Alex Lee ◽  
...  
Keyword(s):  
Anterior Cingulate Cortex ◽  
Glutamate Release ◽  
Critical Role ◽  
Pyramidal Cells ◽  
Cingulate Cortex ◽  
Long Term Potentiation ◽  
Anterior Cingulate ◽  
Cortical Region ◽  
Adult Mice

Abstract Introduction N-Methyl-D-aspartate receptors (NMDARs) play a critical role in different forms of plasticity in the central nervous system. NMDARs are always assembled in tetrameric form, in which two GluN1 subunits and two GluN2 and/or GluN3 subunits combine together. Previous studies focused mainly on the hippocampus. The anterior cingulate cortex (ACC) is a key cortical region for sensory and emotional functions. NMDAR GluN2A and GluN2B subunits have been previously investigated, however much less is known about the GluN2C/2D subunits. Results In the present study, we found that the GluN2C/2D subunits are expressed in the pyramidal cells of ACC of adult mice. Application of a selective antagonist of GluN2C/2D, (2R*,3S*)-1-(9-bromophenanthrene-3-carbonyl) piperazine-2,3-dicarboxylic acid (UBP145), significantly reduced NMDAR-mediated currents, while synaptically evoked EPSCs were not affected. UBP145 affected neither the postsynaptic long-term potentiation (post-LTP) nor the presynaptic LTP (pre-LTP). Furthermore, the long-term depression (LTD) was also not affected by UBP145. Finally, both UBP145 decreased the frequency of the miniature EPSCs (mEPSCs) while the amplitude remained intact, suggesting that the GluN2C/2D may be involved in presynaptic regulation of spontaneous glutamate release. Conclusions Our results provide direct evidence that the GluN2C/2D contributes to evoked NMDAR mediated currents and mEPSCs in the ACC, which may have significant physiological implications.

scholarly journals Neonatal ketamine exposure causes impairment of long-term synaptic plasticity in the anterior cingulate cortex of rats

Neuroscience ◽  
2014 ◽  
Vol 268 ◽  
pp. 309-317 ◽  
Author(s):  
R.-R. Wang ◽  
J.-H. Jin ◽  
A.W. Womack ◽  
D. Lyu ◽  
S.S. Kokane ◽  
...  
Keyword(s):  
Synaptic Plasticity ◽  
Anterior Cingulate Cortex ◽  
Cingulate Cortex ◽  
Anterior Cingulate

scholarly journals NMDA Receptor-Dependent Synaptic Depression in Potentiated Synapses of the Anterior Cingulate Cortex of adult Mice

2021 ◽  
Vol 17 ◽  
pp. 174480692110180
Author(s):  
Man Xue ◽  
Si-Bo Zhou ◽  
Ren-Hao Liu ◽  
Qi-Yu Chen ◽  
Min Zhuo ◽  
...  
Keyword(s):  
Chronic Pain ◽  
Nmda Receptor ◽  
Anterior Cingulate Cortex ◽  
Cingulate Cortex ◽  
Synaptic Depression ◽  
Anterior Cingulate ◽  
Dependent Manner ◽  
Selective Antagonist ◽  
Adult Mice

Long-term potentiation (LTP) is an important molecular mechanism for chronic pain in the anterior cingulate cortex (ACC), a key cortical region for pain perception and emotional regulation. Inhibiting ACC LTP via various manipulations or pharmacological treatments blocks chronic pain. Long-term depression (LTD) is another form of synaptic plasticity in the ACC, which is also proved to be involved in the mechanisms of chronic pain. However, less is known about the interactive relationship between LTP and LTD in the ACC. Whether the synaptic depression could be induced after synaptic LTP in the ACC is not clear. In the present study, we used multi-channel field potential recording systems to study synaptic depression after LTP in the ACC of adult mice. We found that low frequency stimulus (LFS: 1 Hz, 15 min) inhibited theta burst stimulation (TBS)-induced LTP at 30 min after the induction of LTP. However, LFS failed to induce depression at 90 min after the induction of LTP. Furthermore, NMDA receptor antagonist AP-5 blocked the induction of synaptic depression after potentiation. The GluN2B-selective antagonist Ro25-6981 also inhibited the phenomenon in the ACC, while the GluN2A-selective antagonist NVP-AAM077 and the GluN2C/D-selective antagonist PPDA and UBP145 had no any significant effect. These results suggest that synaptic LTP can be depressed by LTD in a time dependent manner, and GluN2B-containing NMDA receptors play important roles in this form of synaptic depression.

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