Vascular parameters of normal cynomolgus macaques and healthy humans obtained by fundus fluorescence angiography and optical coherence tomography angiography
Abstract Background: To provide and compare normal vascular parameters for cynomolgus macaques and healthy humans, compare the advantages and disadvantages of fundus fluorescence angiography (FFA) and optical coherence tomography angiography (OCT-A) in angiography, and establish an eye parameter database for cynomolgus macaques and healthy humans. Methods: 5 normal cynomolgus macaques with a mean age of 4.60±0.55 years were studied for data collection. A Heidelberg Spectralis® HRA+OCT was used to obtain parameters for FFA. The macula of 28 healthy humans (14 males and 14 females), with a mean age of 25.11±6.21 years and the optic discs of 9 healthy humans (4 males and 5 females) with a mean age of 28.56±6.78 years were measured. The vessel density (VD) was measured using an RTVue XR with AngioVue. The scan sizes of the macula and optic discs were 3×3 mm and 4.5×4.5 mm, respectively. Results: FFA of cynomolgus macaques revealed stages similar to those observed in humans. OCT-A was used to image the superficial and deep capillary plexuses and radial peripapillary capillary network. The highest entire en-face mean VD in the macular area was 68.19±0.75% in the choroid capillary layer. In both layers of the optic disc, the VD in the nasal quadrant was lower than the VD in the inferior temporal quadrant. Similarities and significant differences in VD between healthy humans and cynomolgus macaques were obtained using OCT-A. Conclusions: This study provides normal vascular parameters for cynomolgus macaques using FFA and OCT-A to help establish an optical parameter database for cynomolgus macaques and compare VD between healthy humans and cynomolgus macaques to promote choroid-retinopathy research. Trial registration: Current Controlled Trials NCT03692169