scholarly journals Differentiation of human induced-pluripotent stem cells into smooth muscle cells

2021 ◽  
Author(s):  
Patricia R. Pitrez ◽  
Lino Ferreira
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Muscle Cells ◽  
Wild Type ◽  
Induced Pluripotent ◽  
Progeria Syndrome ◽  
Hutchinson Gilford Progeria Syndrome

Abstract Here we describe a protocol for the generation of SMCs from Hutchinson-Gilford Progeria Syndrome (HGPS)- induced pluripotent stem cells (iPSCs) and wild type iPSCs to study their vulnerability.

scholarly journals Vascular Smooth Muscle Cells From Hypertensive Patient-Derived Induced Pluripotent Stem Cells to Advance Hypertension Pharmacogenomics

2015 ◽  
Vol 4 (12) ◽  
pp. 1380-1390 ◽  
Author(s):  
Nikolett M. Biel ◽  
Katherine E. Santostefano ◽  
Bayli B. DiVita ◽  
Nihal El Rouby ◽  
Santiago D. Carrasquilla ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Hypertensive Patient ◽  
Induced Pluripotent Stem Cells ◽  
Vascular Smooth Muscle Cells ◽  
Pluripotent Stem Cells ◽  
Muscle Cells ◽  
Induced Pluripotent

Abstract 13982: Derivation of Functional Smooth Muscle Cells From Integration-free Induced Pluripotent Stem Cells Through Cardiovascular Progenitor Cell Intermediates for Vascular Regeneration

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Yongyu Wang ◽  
Jiang Hu ◽  
Jiao jiao ◽  
Zhou Zhou ◽  
Eugene Y Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Progenitor Cell ◽  
Muscle Cells ◽  
Vascular Regeneration ◽  
Induced Pluripotent

Tissue-engineered blood vessels (TEBVs) hold great promise for replacement of damaged or defective vascular tissues in vascular disease therapies, such as coronary and peripheral bypass graft surgeries. However, it remains a great challenge to obtain sufficient numbers of functional smooth muscle cells (SMCs) in the practice of constructing patient-specific TEBVs. This study aimed to develop an efficient method to generate a large number of functional SMCs in a short term for constructing tissue-engineered vascular tissues. Human induced pluripotent stem cells (iPSCs) were established by integration-free episomal vector-based reprogramming of donor peripheral blood mononuclear cells (PBMCs). These established iPSCs expressed pluripotency markers and were demonstrated to be able to differentiate into all three germ layer cells. Cardiovascular progenitor cell (CVPC) intermediates were then promptly and efficiently induced and expanded in chemically defined medium. Vascular smooth muscle cells (SMCs) were further induced under differentiation condition, which expressed typical SMCs markers including smooth muscle α-actin (α-SMA), calponin and SM22α validated by quantitative real-time PCR and immunocytochemistry stain. Importantly, the derived SMCs showed functional properties, validated by contraction responsiveness to carbachol treatment, up-regulation of specific collagens gene expression under transforming growth factor β1 treatment and up-regulation of specific matrix metalloproteinases gene expression under cytokine stimuli. Future studies will be focused on using these functional SMCs to construct functional TEBVs on biomimetic scaffolds. Taken together, our study established a facile procedure to generate large amount of functional and safe SMCs for vascular regeneration, via establishment of donor-specific integration-free human iPSCs and directed differentiation through CVPC intermediates.

scholarly journals A Comparison of Murine Smooth Muscle Cells Generated from Embryonic versus Induced Pluripotent Stem Cells

2009 ◽  
Vol 18 (5) ◽  
pp. 741-748 ◽  
Author(s):  
Chang-Qing Xie ◽  
Huarong Huang ◽  
Sheng Wei ◽  
Long-Sheng Song ◽  
Jifeng Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Muscle Cells ◽  
Induced Pluripotent

scholarly journals Generation of Endothelial and Smooth Muscle Cells from Werner Syndrome-Specific Induced Pluripotent Stem Cells

2018 ◽  
Vol 64 (3) ◽  
pp. 207-215
Author(s):  
GAKUTO YOZU ◽  
SHINSUKE YUASA ◽  
SHUGO TOHYAMA ◽  
TOMOHISA SEKI ◽  
MINORU TAKEMOTO ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Werner Syndrome ◽  
Muscle Cells ◽  
Induced Pluripotent

scholarly journals Differentiation of Human Induced-Pluripotent Stem Cells into Smooth-Muscle Cells: Two Novel Protocols

PLoS ONE ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. e0147155 ◽  
Author(s):  
Libang Yang ◽  
Zhaohui Geng ◽  
Thomas Nickel ◽  
Caitlin Johnson ◽  
Lin Gao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Muscle Cells ◽  
Induced Pluripotent

scholarly journals Derivation of Smooth Muscle Cells with Neural Crest Origin from Human Induced Pluripotent Stem Cells

2012 ◽  
Vol 195 (1-2) ◽  
pp. 5-14 ◽  
Author(s):  
Aijun Wang ◽  
Zhenyu Tang ◽  
Xian Li ◽  
Yisu Jiang ◽  
Danielle A. Tsou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Neural Crest ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Muscle Cells ◽  
Neural Crest Origin ◽  
Induced Pluripotent
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