scholarly journals Detection of anti-SARS-CoV-2 antibodies in patients with rheumatoid arthritis.

2020 ◽  
Author(s):  
Shomi Oka ◽  
Takashi Higuchi ◽  
Hiroshi Furukawa ◽  
Kota Shimada ◽  
Atsushi Hashimoto ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Serological Test ◽  
Rheumatoid Factors ◽  
National Hospital ◽  
Serum Samples ◽  
Igg Rheumatoid Factor ◽  
Electrochemiluminescence Immunoassay ◽  
N 93 ◽  
Prior Infection

Abstract Objectives: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19) and the outbreak of COVID-19 was reported in December 2019 in Wuhan, China. Serological test is conducted to discriminate prior infection of SARS-CoV-2. The influence of auto-antibodies on the results of anti-SARS-CoV-2 antibodies was investigated in a few studies. Here, we investigated whether the results of anti-SARS-CoV-2 antibodies would be modified in patients with rheumatoid arthritis (RA). Methods: Patients with RA were recruited at Sagamihara National Hospital from July 2014 to October 2015 (n=38, 2014 cohort) and at Tokyo National Hospital from June to October 2020 (n=93, 2020 cohort). Anti-SARS-CoV-2 antibodies were measured in collected sera from these RA patients by electrochemiluminescence immunoassay (ECLIA) or immunochromatographic assay (ICA). Results: Anti-SARS-CoV-2 antibodies were not detected in all the samples form RA patients in both cohorts by the ECLIA. However, anti-SARS-CoV-2 antibodies were detected in the serum samples from three (7.9%) in 2014 cohort by the ICA and fifteen (16.1%) in 2020 cohort. The IgM rheumatoid factor levels were increased in RA patients with IgM anti-SARS-CoV-2 antibodies by ICA compared with RA without any anti-SARS-CoV-2 antibodies (mean ± standard deviation [IU/ml], 1223.0 ± 1308.7 vs. 503.6 ± 1947.2, P=0.0101). The levels of IgG rheumatoid factor were also upregulated in RA patients with IgM anti-SARS-CoV-2 antibodies by ICA (4.0 ± 0.7 vs. 2.4 ± 0.9, P=0.0013). Conclusion: The results of IgM anti-SARS-CoV-2 antibody by the ICA would be modified by IgM or IgG rheumatoid factors in RA patients.

OCCURRENCE OF γ-GLOBULIN COMPLEXES IN SERUM AND JOINT FLUID OF RHEUMATOID ARTHRITIS PATIENTS: USE OF MONOCLONAL RHEUMATOID FACTORS AS REAGENTS FOR THEIR DEMONSTRATION

1971 ◽  
Vol 134 (3) ◽  
pp. 286-295 ◽  
Author(s):  
R. J. Winchester ◽  
H. G. Kunkel ◽  
V. Agnello
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Lupus Erythematosus ◽  
Complement Fixation ◽  
Joint Fluid ◽  
Exact Nature ◽  
Rheumatoid Factors ◽  
Serum Complement ◽  
Systemic Lupus ◽  
Low Incidence

γG globulin complexed in an unusual form has been demonstrated in the serum of many patients with rheumatoid arthritis. Such complexes have been detected and isolated principally through precipitation reactions with monoclonal γM rheumatoid factors. These monoclonal rheumatoid factors exhibited a greater sensitivity to react with small complexes or aggregates of γ-globulin than polyclonal rheumatoid factor from rheumatoid arthritis sera or isolated C1q. The serum complexes consisted in large part of high molecular weight but acid-dissociable 7S γG globulin molecules They however differed from the complexes in the joint fluid by not yielding precipitates with C1q and were not found in association with evidence of marked serum complement fixation or activation. A small number of systemic lupus erythematosus sera, primarily those forming cryoprecipitates, also gave reactions with monoclonal rheumatoid factor. Sera from patients with a variety of nonrheumatic diseases gave a low incidence of reactions. The exact nature of the complexes in the rheumatoid arthritis sera remains somewhat in doubt although γG rheumatoid factors appear partly involved.

A Subgroup of Rheumatoid Arthritis (RA) Associated with Selective Binding of Rheumatoid Factors to Antigen Structures of Cytomegalovirus (CMV)

1988 ◽  
Vol 1 (3) ◽  
pp. 211-218
Author(s):  
P. Peichl ◽  
G. Haberhauer ◽  
M. Scriba ◽  
H. Bröll
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Binding Capacity ◽  
Specific Binding ◽  
Virus Antigen ◽  
Rheumatoid Factors ◽  
Cmv Infection ◽  
Selective Binding ◽  
Displacement Reactions ◽  
Pathogenetic Factor

We compared antibodies (Ab) with both, antiviral and anti IgG properties in patients with rheumatoid arthritis with malignant progress, short remissions and no improvement to therapy. The selective binding of Rheumatoid Factor (RF) to antigen structures of CMV were tested in 180 patients by a newly established ELISA with antiidiotypic goat antibodies of CMV neutralizing human monoclonal Ab and against the virus antigen directly. Displacement reactions on the idiotypic bindingsite as well as comparative ELISA gave proof of the special RF activity. 24,2% of the patients suffering from RA, with IgM RF, showed selective binding to antigen structures of CMV and anti IgG activity. The evidence of the specific binding capacity of RF to the virus antigen in chronic CMV infection as well as their defined binding capacity to the Fc Fragment of IgG suggest CMV specific RF as the pathogenetic factor of malignant forms of inflammatory rheumatism.

IgG-rheumatoid factor in seronegative rheumatoid arthritis

1983 ◽  
Vol 12 (sup52) ◽  
pp. 79-79
Author(s):  
T. Palosuo ◽  
K. Aho ◽  
V. Raunio ◽  
K. Kaarela
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Igg Rheumatoid Factor

IgG RHEUMATOID FACTOR IN PURIFIED IgG FRACTIONS AND WHOLE SERA FROM PATIENTS WITH RHEUMATOID ARTHRITIS

Rheumatology ◽  
1989 ◽  
Vol 28 (3) ◽  
pp. 227-232
Author(s):  
N. HARATS ◽  
A. RUBINOW ◽  
R. FISCHEL ◽  
D. EILAT
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Igg Rheumatoid Factor

scholarly journals Seronegative rheumatoid arthritis, rheumatoid factor cross reactive idiotype expression, and hidden rheumatoid factors.

1989 ◽  
Vol 48 (6) ◽  
pp. 488-495 ◽  
Author(s):  
V R Bonagura ◽  
J F Wedgwood ◽  
N Agostino ◽  
L Hatam ◽  
L Mendez ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Rheumatoid Factors ◽  
Arthritis Rheumatoid

scholarly journals IgG Rheumatoid Factor in Synovial Fluid of Patients with Rheumatoid Arthritis.

1998 ◽  
Vol 47 (2) ◽  
pp. 693-697
Author(s):  
Minoru Ikeda ◽  
Toshio Inoue ◽  
Takashi Ishinishi ◽  
Kosuke Ogata
Keyword(s):  
Rheumatoid Arthritis ◽  
Synovial Fluid ◽  
Rheumatoid Factor ◽  
Igg Rheumatoid Factor

scholarly journals Soluble selectins and highly fucosylated ?1-antichymotrypsin in rheumatoid arthritis patients

2015 ◽  
Vol 84 (1) ◽  
pp. 34-40
Author(s):  
Anna Olewicz-Gawlik ◽  
Izabela Korczowska-Łącka ◽  
Paweł Hrycaj
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Acute Phase Proteins ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Serum Concentrations ◽  
Serum Samples ◽  
Male And Female ◽  
Leukocyte Extravasation

Introduction. Fucosylation of acute phase proteins and serum soluble selectin levels is increased in rheumatoid arthritis (RA) patients and can influence leukocyte extravasation. Aim. The aim of this study was to evaluate the concentration and fucosylation of ?1-antichymotrypsin (ACT) in relation to serum concentrations of soluble forms of selectins in RA patients. Material and methods. Serum samples of 70 RA patients and 30 healthy controls were examined using sandwich enzyme-linked immunosorbent assay (ELISA). Results. ACT-FR was significantly increased in RA patients when compared to healthy controls (p < 0.001) and significantly correlated with serum concentrations of rheumatoid factor (RF) and antibodies against cyclic citrullinated peptides (ACPA) (p = 0.006, p = 0.04, respectively). Moreover, we found significant correlations between the serum levels of soluble (s)P- and sE-selectin and ACT-FR (p = 0.008 and p = 0.03, respectively) only in male RA patients.Conclusions. Fucosylation of ACT differs between male and female RA patients and is related to sP- and sE-selectin levels only in men.

scholarly journals Increased IgG Rheumatoid Factor-Positivity in the Asian Rheumatoid Arthritis Patients Irrespective of Ethnicity

2014 ◽  
Vol 04 (01) ◽  
pp. 43-51 ◽  
Author(s):  
Chun Lai Too ◽  
Johan Rönnelid ◽  
Yuslina Mat Yusoff ◽  
Jasbir Singh Dhaliwal ◽  
Nor Ashikin Jinah ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Igg Rheumatoid Factor
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