A Subgroup of Rheumatoid Arthritis (RA) Associated with Selective Binding of Rheumatoid Factors to Antigen Structures of Cytomegalovirus (CMV)

1988 ◽  
Vol 1 (3) ◽  
pp. 211-218
Author(s):  
P. Peichl ◽  
G. Haberhauer ◽  
M. Scriba ◽  
H. Bröll
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Binding Capacity ◽  
Specific Binding ◽  
Virus Antigen ◽  
Rheumatoid Factors ◽  
Cmv Infection ◽  
Selective Binding ◽  
Displacement Reactions ◽  
Pathogenetic Factor

We compared antibodies (Ab) with both, antiviral and anti IgG properties in patients with rheumatoid arthritis with malignant progress, short remissions and no improvement to therapy. The selective binding of Rheumatoid Factor (RF) to antigen structures of CMV were tested in 180 patients by a newly established ELISA with antiidiotypic goat antibodies of CMV neutralizing human monoclonal Ab and against the virus antigen directly. Displacement reactions on the idiotypic bindingsite as well as comparative ELISA gave proof of the special RF activity. 24,2% of the patients suffering from RA, with IgM RF, showed selective binding to antigen structures of CMV and anti IgG activity. The evidence of the specific binding capacity of RF to the virus antigen in chronic CMV infection as well as their defined binding capacity to the Fc Fragment of IgG suggest CMV specific RF as the pathogenetic factor of malignant forms of inflammatory rheumatism.

OCCURRENCE OF γ-GLOBULIN COMPLEXES IN SERUM AND JOINT FLUID OF RHEUMATOID ARTHRITIS PATIENTS: USE OF MONOCLONAL RHEUMATOID FACTORS AS REAGENTS FOR THEIR DEMONSTRATION

1971 ◽  
Vol 134 (3) ◽  
pp. 286-295 ◽  
Author(s):  
R. J. Winchester ◽  
H. G. Kunkel ◽  
V. Agnello
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Lupus Erythematosus ◽  
Complement Fixation ◽  
Joint Fluid ◽  
Exact Nature ◽  
Rheumatoid Factors ◽  
Serum Complement ◽  
Systemic Lupus ◽  
Low Incidence

γG globulin complexed in an unusual form has been demonstrated in the serum of many patients with rheumatoid arthritis. Such complexes have been detected and isolated principally through precipitation reactions with monoclonal γM rheumatoid factors. These monoclonal rheumatoid factors exhibited a greater sensitivity to react with small complexes or aggregates of γ-globulin than polyclonal rheumatoid factor from rheumatoid arthritis sera or isolated C1q. The serum complexes consisted in large part of high molecular weight but acid-dissociable 7S γG globulin molecules They however differed from the complexes in the joint fluid by not yielding precipitates with C1q and were not found in association with evidence of marked serum complement fixation or activation. A small number of systemic lupus erythematosus sera, primarily those forming cryoprecipitates, also gave reactions with monoclonal rheumatoid factor. Sera from patients with a variety of nonrheumatic diseases gave a low incidence of reactions. The exact nature of the complexes in the rheumatoid arthritis sera remains somewhat in doubt although γG rheumatoid factors appear partly involved.

scholarly journals Seronegative rheumatoid arthritis, rheumatoid factor cross reactive idiotype expression, and hidden rheumatoid factors.

1989 ◽  
Vol 48 (6) ◽  
pp. 488-495 ◽  
Author(s):  
V R Bonagura ◽  
J F Wedgwood ◽  
N Agostino ◽  
L Hatam ◽  
L Mendez ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Rheumatoid Factors ◽  
Arthritis Rheumatoid

scholarly journals Detection of anti-SARS-CoV-2 antibodies in patients with rheumatoid arthritis.

2020 ◽  
Author(s):  
Shomi Oka ◽  
Takashi Higuchi ◽  
Hiroshi Furukawa ◽  
Kota Shimada ◽  
Atsushi Hashimoto ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Serological Test ◽  
Rheumatoid Factors ◽  
National Hospital ◽  
Serum Samples ◽  
Igg Rheumatoid Factor ◽  
Electrochemiluminescence Immunoassay ◽  
N 93 ◽  
Prior Infection

Abstract Objectives: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19) and the outbreak of COVID-19 was reported in December 2019 in Wuhan, China. Serological test is conducted to discriminate prior infection of SARS-CoV-2. The influence of auto-antibodies on the results of anti-SARS-CoV-2 antibodies was investigated in a few studies. Here, we investigated whether the results of anti-SARS-CoV-2 antibodies would be modified in patients with rheumatoid arthritis (RA). Methods: Patients with RA were recruited at Sagamihara National Hospital from July 2014 to October 2015 (n=38, 2014 cohort) and at Tokyo National Hospital from June to October 2020 (n=93, 2020 cohort). Anti-SARS-CoV-2 antibodies were measured in collected sera from these RA patients by electrochemiluminescence immunoassay (ECLIA) or immunochromatographic assay (ICA). Results: Anti-SARS-CoV-2 antibodies were not detected in all the samples form RA patients in both cohorts by the ECLIA. However, anti-SARS-CoV-2 antibodies were detected in the serum samples from three (7.9%) in 2014 cohort by the ICA and fifteen (16.1%) in 2020 cohort. The IgM rheumatoid factor levels were increased in RA patients with IgM anti-SARS-CoV-2 antibodies by ICA compared with RA without any anti-SARS-CoV-2 antibodies (mean ± standard deviation [IU/ml], 1223.0 ± 1308.7 vs. 503.6 ± 1947.2, P=0.0101). The levels of IgG rheumatoid factor were also upregulated in RA patients with IgM anti-SARS-CoV-2 antibodies by ICA (4.0 ± 0.7 vs. 2.4 ± 0.9, P=0.0013). Conclusion: The results of IgM anti-SARS-CoV-2 antibody by the ICA would be modified by IgM or IgG rheumatoid factors in RA patients.

Concanavalin A-induced Aggregation of Human Blood Platelets

1975 ◽  
Vol 33 (02) ◽  
pp. 354-360 ◽  
Author(s):  
Heinrich Patscheke ◽  
Reinhard Brossmer
Keyword(s):  
Concanavalin A ◽  
Binding Capacity ◽  
Specific Binding ◽  
Blood Platelets ◽  
Residual Effect ◽  
Independent Effect ◽  
Con A ◽  
Experimental Conditions ◽  
Main Effect ◽  
Induced Aggregation

SummaryConcanavalin A (CON A) causes platelets to aggregate. A Ca++-independent effect of CON A could be separated from a main effect which depends on Ca++. The main effect probably is a consequence of the CON A-induced platelet release reaction and therefore is platelet-specific. The weak residual effect observed in the presence of Na2EDTA may be due to a similar mechanism as has been demonstrated for CON A-induced aggregations of several other normal and malignant transformed animal cells.Na2EDTA did not inhibit the carbohydrate-specific binding capacity of CON A. Therefore, Na2EDTA appears not to demineralize the CON A molecules under these experimental conditions.α-methyl-D-glucoside inhibits the Ca++-independent as well as the Ca++-dependent effect of CON A.Pretreatment by neuraminidase stimulated the platelet aggregation induced by CON A. It is possible that removal of terminal sialic acid residues makes additional receptors accessible for the binding of CON A.

Relationship of HLA-DRB1 Alleles and Rheumatoid Arthritis in West South of Iran

2017 ◽  
Vol 9 (03) ◽  
Author(s):  
Karim Mowla ◽  
Elham Rajaee M. D. ◽  
Mehrdad Dargahi-MalAmir M. D. ◽  
Neda Yousefinezhad ◽  
Maryam Jamali Hondori
Keyword(s):  
Rheumatoid Arthritis ◽  
Environmental Factors ◽  
Rheumatoid Factor ◽  
Dna Extraction ◽  
Wrist Joint ◽  
Blood Sampling ◽  
Healthy Controls ◽  
Relationship Of ◽  
Synovial Pannus

Background: Rheumatoid arthritis is a systemic multifactor disease that presented with symmetrical polyarthritis more preferably in small wrist joint and ankle. Synovial pannus cause destruction and deformities in joints. The main reason of this disease in unknown, but past researchesshowed that genetically factor play important role beside environmental factors in susceptibility to this entity. Method:100 patients with rheumatoid arthritis diagnosed upon ACR 2010 criteria enrolled study. 92 healthy patents also enrolled DNA studying. of both group was extracted through DNA extraction kits by blood sampling. HLA-DRB1 typing was done by PCR-SSP method. Results: There were no significant differences in HLADRB1 *04, HLADRB1*08 and HLADRB1*11 alleles presentation between patients and healthy controls. Only there were statically significant correlation between HLA-DRB1*08 and Rheumatoid factor positive patents. (P = 0.025).

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