scholarly journals Overexpression of MpbHLH Transcription Factor, An Encoding ICE-Like Protein, Enhances Foc TR4-Resistance of Cavendish Banana

2021 ◽  
Author(s):  
Haochen Li ◽  
Chunhua Hu ◽  
Aifeng Xie ◽  
Shaoping Wu ◽  
Fangcheng Bi ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transgenic Plants ◽  
Fusarium Wilt ◽  
Phenylpropanoid Pathway ◽  
Isolation And Identification ◽  
Musa Spp ◽  
Cavendish Banana ◽  
Resistant Varieties ◽  
Banana Industry ◽  
Selection Of

Abstract Background: Fusarium wilt is a worldwide problem that restrains the development of banana industry. Isolation and identification of resistance genes is not only significance for the selection of disease resistant varieties, but also important for banana molecular breeding. Results: In this study, an ICE1-like transcription factor containing bHLH domain was cloned from Dajiao (Musa spp. ABB group) and named MpbHLH. We found that overexpression of MpbHLH in Cavendish banana (Musa spp. AAA group) could significantly enhance Fusarium wilt resistance which is caused by Foc TR4. Transcriptomics and proteomic analysis showed that phenylalanine ammonia lyase (PAL) and peroxidase (POD) were highly expressed in transgenic plants. Physiological experiments further revealed that transgenic plants, especially their roots, had high POD activity. Conclusions: Overexpression of MpbHLH up-regulates the phenylpropanoid pathway-related genes and POD-associated genes in transgenic Cavendish banana. These changes may eventually lead to the improvement of disease resistance.

scholarly journals Towards the development of a Cavendish banana resistant to race 4 of fusarium wilt: gamma irradiation of micropropagated Dwarf Parfitt (Musa spp., AAA group, Cavendish subgroup)

2006 ◽  
Vol 46 (1) ◽  
pp. 107 ◽  
Author(s):  
M. K. Smith ◽  
S. D. Hamill ◽  
P. W. Langdon ◽  
J. E. Giles ◽  
V. J. Doogan ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Lower Incidence ◽  
Fruit Size ◽  
Field Resistance ◽  
Mutation Induction ◽  
Industry Standard ◽  
Cavendish Banana ◽  
Gamma Irradiated ◽  
Race 4 ◽  
Selection Of

‘Dwarf Parfitt’, an extra-dwarf Cavendish cultivar with resistance to subtropical race 4 Fusarium oxysporum f. sp. cubense (Foc), was gamma irradiated at a dose of 20 Gy and putative mutants were recovered with improved agronomic characteristics. Further screening of putative mutants for improved yield and fruit size, as well as a degree of resistance to fusarium wilt, led to the selection of a line (DPM25) with improved productivity when grown on soils infested with subtropical race 4 Foc. DPM25 was equal to the industry standard, ‘Williams’, in every agronomic trait measured and it consistently showed a lower incidence of fusarium wilt. Further improvement of field resistance to race 4 Foc is needed in DPM25 and further cycles of mutation induction and selection is an option discussed.

scholarly journals MaMAPK3-MaICE1-MaPOD P7 pathway, a positive regulator of cold tolerance in banana

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jie Gao ◽  
Tongxin Dou ◽  
Weidi He ◽  
Ou Sheng ◽  
Fangcheng Bi ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Cold Tolerance ◽  
Molecular Breeding ◽  
Cold Resistance ◽  
Oxidoreductase Activity ◽  
Tropical Fruit ◽  
Over Expression ◽  
Cavendish Banana ◽  
Cold Tolerant ◽  
Necrotic Symptoms

Abstract Background Banana is a tropical fruit with a high economic impact worldwide. Cold stress greatly affects the development and production of banana. Results In the present study, we investigated the functions of MaMAPK3 and MaICE1 involved in cold tolerance of banana. The effect of RNAi of MaMAPK3 on Dajiao (Musa spp. ‘Dajiao’; ABB Group) cold tolerance was evaluated. The leaves of the MaMAPK3 RNAi transgenic plants showed wilting and severe necrotic symptoms, while the wide-type (WT) plants remained normal after cold exposure. RNAi of MaMAPK3 significantly changed the expressions of the cold-responsive genes, and the oxidoreductase activity was significantly changed in WT plants, while no changes in transgenic plants were observed. MaICE1 interacted with MaMAPK3, and the expression level of MaICE1 was significantly decreased in MaMAPK3 RNAi transgenic plants. Over-expression of MaICE1 in Cavendish banana (Musa spp. AAA group) indicated that the cold resistance of transgenic plants was superior to that of the WT plants. The POD P7 gene was significantly up-regulated in MaICE1-overexpressing transgenic plants compared with WT plants, and the POD P7 was proved to interact with MaICE1. Conclusions Taken together, our work provided new and solid evidence that MaMAPK3-MaICE1-MaPOD P7 pathway positively improved the cold tolerance in monocotyledon banana, shedding light on molecular breeding for the cold-tolerant banana or other agricultural species.

scholarly journals Improved strategies to efficiently isolate thermophilic, thermotolerant, and heat-resistant fungi from compost and soil

2021 ◽  
Vol 20 (3) ◽  
pp. 325-339
Author(s):  
Frederick Witfeld ◽  
Dominik Begerow ◽  
Marco Alexandre Guerreiro
Keyword(s):  
Elevated Temperatures ◽  
Fungal Species ◽  
Dna Barcodes ◽  
Heat Shock Treatment ◽  
Isolation And Identification ◽  
Heat Resistant ◽  
Heat Stable ◽  
Promising Source ◽  
Culture Dependent ◽  
Selection Of

AbstractThermophilic, thermotolerant and heat-resistant fungi developed different physiological traits, enabling them to sustain or even flourish under elevated temperatures, which are life-hostile for most other eukaryotes. With the growing demand of heat-stable molecules in biotechnology and industry, the awareness of heat-adapted fungi as a promising source of respective enzymes and biomolecules is still increasing. The aim of this study was to test two different strategies for the efficient isolation and identification of distinctly heat-adapted fungi from easily accessible substrates and locations. Eight compost piles and ten soil sites were sampled in combination with different culture-dependent approaches to describe suitable strategies for the isolation and selection of thermophilous fungi. Additionally, an approach with a heat-shock treatment, but without elevated temperature incubation led to the isolation of heat-resistant mesophilic species. The cultures were identified based on morphology, DNA barcodes, and microsatellite fingerprinting. In total, 191 obtained isolates were assigned to 31 fungal species, from which half are truly thermophilic or thermotolerant, while the other half are heat-resistant fungi. A numerous amount of heat-adapted fungi was isolated from both compost and soil samples, indicating the suitability of the used approaches and that the richness and availability of those organisms in such environments are substantially high.

JcMYB1, a Jatropha R2R3MYB Transcription Factor Gene, Modulates Lipid Biosynthesis in Transgenic Plants

2018 ◽  
Vol 60 (2) ◽  
pp. 462-475 ◽  
Author(s):  
Kasim Khan ◽  
Vinod Kumar ◽  
Abhishek Niranjan ◽  
Aarti Shanware ◽  
Vidhu A Sane
Keyword(s):  
Transcription Factor ◽  
Transgenic Plants ◽  
Lipid Biosynthesis ◽  
Transcription Factor Gene ◽  
Factor Gene

Identification and evaluation of two diagnostic markers linked to Fusarium wilt resistance (race 4) in banana (Musa spp.)

2011 ◽  
Vol 39 (1) ◽  
pp. 451-459 ◽  
Author(s):  
Wei Wang ◽  
Yulin Hu ◽  
Dequan Sun ◽  
Christian Staehelin ◽  
Dawei Xin ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Diagnostic Markers ◽  
Wilt Resistance ◽  
Musa Spp ◽  
Race 4 ◽  
Fusarium Wilt Resistance

scholarly journals Herbicide-resistant crops in resistant weed management : An industrial perspective

2005 ◽  
Vol 75 (4) ◽  
pp. 79-84 ◽  
Author(s):  
D. Shaner
Keyword(s):  
Weed Control ◽  
Weed Management ◽  
Wild Species ◽  
Integrated Weed Management ◽  
Herbicide Resistant ◽  
Resistant Varieties ◽  
Selection Of

Some of the first products of biotechnology to reach the marketplace have been herbicide-resistant crops. Industry sees the development of herbicide-resistant varieties as a way to increase the availability of proven herbicides for a broader range of crops. However, the development of herbicide- resistant crops requires special attention to potential environmental questions such as herbicide usage, selection of resistant weed biotypes and spread of resistance from the resistant crop to wild species. Industry is actively addressing these concerns during the process of development. Proper development and use of herbicide-resistant crops in integrated weed management programs will provide farmers with increased flexibility, efficiency, and decreased cost in their weed control practices without increasing the risk of herbicide-resistant weeds. Furthermore, herbicide-resistant crops should prove to be valuable tools in managing herbicide- resistant weeds.

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