scholarly journals The Co-Culture of ASCs and EPCs Promotes Wound Healing by Promoting Angiogenesis in Full Thickness Skin Defects

2020 ◽  
Author(s):  
Shuang Lin ◽  
Xiaoning He ◽  
Yuanjia He
Keyword(s):  
Culture System ◽  
Large Scale ◽  
Donor Site ◽  
Skin Tissue ◽  
Skin Defect ◽  
Full Thickness ◽  
Skin Defects ◽  
Full Thickness Skin ◽  
Thickness Skin

Abstract Background The repair of large-scale full-thickness skin defects represents a challenging obstacle in skin tissue engineering. To address the most important problem in skin defect repair, namely insufficient blood supply, this study aimed to find a method that could promote the formation of vascularized skin tissue. Method The phenotypes of ASCs and EPCs were identified respectively, and ASCs/EPCs were co-cultured in vitro to detect the expression of dermal and angiogenic genes. Furthermore, the co-culture system combined with dermal extracellular matrix hydrogel was used to repair the full-scale skin defects in rats.Result The co-culture of ASCs/EPCs could increase skin and angiogenesis-related gene expression in vitro. The results of in vivo animal experiments demonstrated that the ASCs/EPCs group could significantly accelerate the repair of skin defects by promoting the regeneration of vascularized skin.Conclusion It is feasible to replace traditional single seed cells with ASC/EPC co-culture system for vascularized skin regeneration. This system could ultimately enable clinicians to better repair the full-thickness skin defects and avoid donor site morbidity.

scholarly journals Co-culture of ASCs/EPCs and dermal extracellular matrix hydrogel enhances the repair of full-thickness skin wound by promoting angiogenesis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Shuang Lin ◽  
Xiaoning He ◽  
Yuanjia He
Keyword(s):  
Extracellular Matrix ◽  
Culture System ◽  
Large Scale ◽  
Skin Tissue ◽  
Skin Defect ◽  
Full Thickness ◽  
Skin Defects ◽  
Full Thickness Skin ◽  
Thickness Skin

Abstract Background The repair of large-scale full-thickness skin defects represents a challenging obstacle in skin tissue engineering. To address the most important problem in skin defect repair, namely insufficient blood supply, this study aimed to find a method that could promote the formation of vascularized skin tissue. Method The phenotypes of ASCs and EPCs were identified respectively, and ASCs/EPCs were co-cultured in vitro to detect the expression of dermal and angiogenic genes. Furthermore, the co-culture system combined with dermal extracellular matrix hydrogel was used to repair the full-scale skin defects in rats. Result The co-culture of ASCs/EPCs could increase skin- and angiogenesis-related gene expression in vitro. The results of in vivo animal experiments demonstrated that the ASCs/EPCs group could significantly accelerate the repair of skin defects by promoting the regeneration of vascularized skin. Conclusion It is feasible to replace traditional single-seed cells with the ASC/EPC co-culture system for vascularized skin regeneration. This system could ultimately enable clinicians to better repair the full-thickness skin defects and avoid donor site morbidity.

scholarly journals Experimental evaluation of the effectiveness of adipose mesenchymal stem cells in full-thickness skin wounds

2021 ◽  
Vol 9 (2) ◽  
pp. 175-181
Author(s):  
Ilya V. Artsimovich ◽  
Evgenii V. Zinoviev ◽  
Aleksander V. Gostimskii ◽  
Marat S. Asadulaev ◽  
Sergey V. Vissarionov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Control Group ◽  
Skin Defect ◽  
Full Thickness ◽  
Skin Defects ◽  
Full Thickness Skin ◽  
Healing Index ◽  
Thickness Skin

BACKGROUND: Currently, numerous techniques or medical devices that allow complete or partial restoration of the lost skin within a short time remain as subjects of development. Many studies have shown the effectiveness of using stem cells in the treatment of full-thickness skin defects, but their use remains very limited. At present, there is no consensus among researchers about the advisability of the use of stem cells in the treatment of burns as well as about the method of their introduction. AIM: This study aimed to examine the possibility of accelerating the reparative histogenesis of tissues in the zone of deep skin burns using cultures of adipogenic mesenchymal cells, as well as to evaluate the effectiveness of various methods of introducing cultures of these cells into the defect zone. MATERIALS AND METHODS: An experimental study was carried out on small laboratory animals (rats). After simulating a grade III burn, mesenchymal stem cells were transplanted and superficially applied to the wound surface or injected under the fascia. In the control group, no wound treatment was performed. To assess the effectiveness of the wound-healing preparations, the appearance of wounds was assessed daily, the nature of the discharge and presence and type of granulations were noted, and the timing of scab rejection and wound healing was recorded. The wound area was assessed using a planimetric method. A histological examination of wound biopsies was carried out on days 7, 14, 21, and 28 of observation. RESULTS: The application of adipogenic mesenchymal stem cells demonstrated the greatest efficiency on the developed burn model. Subfascial administration was less effective, but this method achieved a significant acceleration of wound healing in comparison with the control group. An increase in the healing index by 56.6% demonstrates the highest intensity of reparative regeneration in animals applied with adipogenic mesenchymal stem cells. CONCLUSIONS: The preliminary results show that the application of adipogenic mesenchymal stem cells on the skin defect is more effective than subfascial administration based on the healing index. The use of adipogenic mesenchymal stem cells may substantially increase the effectiveness of the treatment of full-thickness skin defects.

scholarly journals Multi-faceted enhancement of full-thickness skin wound healing by treatment with autologous micro skin tissue columns

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Christiane Fuchs ◽  
Linh Pham ◽  
Jermaine Henderson ◽  
Katherine J. Stalnaker ◽  
R. Rox Anderson ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Donor Site ◽  
Skin Grafting ◽  
Donor Site Morbidity ◽  
Skin Tissue ◽  
Full Thickness ◽  
Impaired Wound Healing ◽  
Full Thickness Skin ◽  
Thickness Skin

AbstractImpaired wound healing is an immense medical challenge, and while autologous skin grafting remains the “gold-standard” therapeutic option for repairing wounds that cannot be closed by primary or secondary intention, it is limited by substantial donor site morbidity. We previously developed the alternative approach of harvesting full-thickness skin tissue in the form of “micro skin tissue columns” (MSTCs), without causing scarring or any other long-term morbidity. In this study we investigated how MSTC treatment affects the different cellular processes involved in wound healing. We found that MSTC-derived cells were able to remodel and repopulate the wound volume, and positively impact multiple aspects of the wound healing process, including accelerating re-epithelialization by providing multiple cell sources throughout the wound area, increasing collagen deposition, enhancing dermal remodeling, and attenuating the inflammatory response. These effects combined to enhance both epidermal and dermal wound healing. This MSTC treatment approach was designed for practical clinical use, could convey many benefits of autologous skin grafting, and avoids the major drawback of donor site morbidity.

Medial Canthal Reconstruction of Skin Defects With Full-Thickness Skin Grafts

2021 ◽  
Vol 47 (8) ◽  
pp. 1135-1137
Author(s):  
Frank W. de Jongh ◽  
Sjaak Pouwels ◽  
Christien Weenen ◽  
Willem A. van den Bosch ◽  
Dion Paridaens
Keyword(s):  
Skin Grafts ◽  
Full Thickness ◽  
Skin Defects ◽  
Full Thickness Skin ◽  
Thickness Skin

scholarly journals The Use of Matriderm and Autologous Skin Graft in the Treatment of Full Thickness Skin Defects

2014 ◽  
Vol 41 (4) ◽  
pp. 330 ◽  
Author(s):  
Jang Hwan Min ◽  
In Sik Yun ◽  
Dae Hyun Lew ◽  
Tai Suk Roh ◽  
Won Jai Lee
Keyword(s):  
Skin Graft ◽  
Full Thickness ◽  
Skin Defects ◽  
Full Thickness Skin ◽  
Thickness Skin

Skin Grafting

2019 ◽  
pp. 65-72
Author(s):  
Stephen M. Milner
Keyword(s):  
Skin Grafting ◽  
Skin Grafts ◽  
Full Thickness ◽  
Preoperative Preparation ◽  
Scar Contracture ◽  
Skin Defects ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Contracture Release

Skin grafting is an indispensable technique used in a variety of clinical situations, including acute burns, traumatic wounds, scar contracture release, and oncological and congenital deficiencies. The author’s preferred techniques for harvesting and resurfacing various skin defects using split- and full-thickness skin grafts are described in this chapter, together with the assessment of donor and recipient sites, preoperative preparation and postoperative considerations.

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