Visualization of the Human Enteric Nervous System by Probe Confocal Laser Endomicroscopy: A First Real Time Observation of Hirschsprung’s Disease and Allied Disorders.

2021 ◽  
Author(s):  
Atsushi Harada ◽  
Naoki Shimojima ◽  
Masakuni Kobayashi ◽  
Shunsuke Kamba ◽  
Seiichi Hirobe ◽  
...  
Keyword(s):  
Nervous System ◽  
Enteric Nervous System ◽  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Cresyl Violet ◽  
Confocal Laser Endomicroscopy ◽  
Confocal Laser ◽  
Time Observation ◽  
Real Time Observation

Abstract Background: Our group previously proved that the human enteric nervous system can be visualized with confocal laser endomicroscopy after topical application of cresyl violet using surgically resected intestine specimens. The present report documents the first in vivo visualization of the human enteric nervous system with confocal laser endomicroscopy using local cresyl violet staining. The aim of this study was to evaluate the technical feasibility and clinical efficiency of confocal laser endomicroscopy in patients with Hirschsprung’s disease and allied disorders in vivo.Methods: Confocal laser endomicroscopy was performed in vivo in two patients to confirm the presence of the enteric nervous system during surgery in patients with Hirschsprung’s disease and allied disorders. Cresyl violet was gently injected from the serosal side into the muscular layer of the intestine, and scanning was performed within 30 minutes. Then, the scanned intestines were resected, and the visualized area of the specimens was pathologically evaluated.Results: The ganglion cell nuclei and the enteric nervous system network were clearly visualized intraoperatively in both cases. The morphological findings were similar to the pathological findings of the enteric nervous system in both cases although the period of visibility was brief. Conclusion: This study demonstrated the first, real-time observation of the enteric nervous system in humans using confocal laser endomicroscopy and the technical feasibility of visualizing the enteric nervous system in patients with Hirschsprung’s disease and allied disorders.

scholarly journals Visualization of the human enteric nervous system by probe confocal laser endomicroscopy: a first real-time observation of Hirschsprung’s disease and allied disorders

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Atsushi Harada ◽  
Naoki Shimojima ◽  
Masakuni Kobayashi ◽  
Shunsuke Kamba ◽  
Seiichi Hirobe ◽  
...  
Keyword(s):  
Nervous System ◽  
Enteric Nervous System ◽  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Cresyl Violet ◽  
Confocal Laser Endomicroscopy ◽  
Confocal Laser ◽  
Time Observation ◽  
Real Time Observation

Abstract Background Our group previously proved that the human enteric nervous system can be visualized with confocal laser endomicroscopy after topical application of cresyl violet using surgically resected intestine specimens. The present report documents the first in vivo visualization of the human enteric nervous system with confocal laser endomicroscopy using local cresyl violet staining. The aim of this study was to evaluate the technical feasibility and clinical efficiency of confocal laser endomicroscopy in patients with Hirschsprung’s disease and allied disorders in vivo. Methods Confocal laser endomicroscopy was performed in vivo in two patients to confirm the presence of the enteric nervous system during surgery in patients with Hirschsprung’s disease and allied disorders. Cresyl violet was gently injected from the serosal side into the muscular layer of the intestine, and scanning was performed within 30 min. Then, the scanned intestines were resected, and the visualized area of the specimens was pathologically evaluated. Results The ganglion cell nuclei and the enteric nervous system network were clearly visualized intraoperatively in both cases. The morphological findings were similar to the pathological findings of the enteric nervous system in both cases although the period of visibility was brief. Conclusion This study demonstrated the first, real-time observation of the enteric nervous system in humans using confocal laser endomicroscopy and suggest the potential to identify the enteric nervous system intra-operatively during surgery for Hirschsprung’s disease and allied disorders.

51 Enteric Nervous System Visualization of Hirschsprung's Disease Using Confocal Laser Endomicroscopy: Ex Vivo Human Trial

2017 ◽  
Vol 85 (5) ◽  
pp. AB44
Author(s):  
Masakuni Kobayashi ◽  
Naoki Shimojima ◽  
Shunsuke Kamba ◽  
Junko Takahashi-Fujigasaki ◽  
Kazuki Sumiyama
Keyword(s):  
Nervous System ◽  
Enteric Nervous System ◽  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Ex Vivo ◽  
Confocal Laser Endomicroscopy ◽  
Confocal Laser ◽  
Human Trial

In vivo imaging of porcine gastric enteric nervous system using confocal laser endomicroscopy &molecular neuronal probe

2016 ◽  
Vol 31 (4) ◽  
pp. 802-807 ◽  
Author(s):  
Jason B. Samarasena ◽  
Amrita Ahluwalia ◽  
Susumu Shinoura ◽  
Kee Don Choi ◽  
John G. Lee ◽  
...  
Keyword(s):  
Nervous System ◽  
Enteric Nervous System ◽  
In Vivo Imaging ◽  
Confocal Laser Endomicroscopy ◽  
Confocal Laser

EUS-guided in vivo imaging of the porcine esophageal enteric nervous system by using needle-based confocal laser endomicroscopy

2015 ◽  
Vol 82 (6) ◽  
pp. 1116-1120 ◽  
Author(s):  
Jason B. Samarasena ◽  
Andrzej S. Tarnawski ◽  
Amrita Ahluwalia ◽  
Susumu Shinoura ◽  
Kee Don Choi ◽  
...  
Keyword(s):  
Nervous System ◽  
Enteric Nervous System ◽  
In Vivo Imaging ◽  
Confocal Laser Endomicroscopy ◽  
Confocal Laser

scholarly journals “Too much guts and not enough brains”: (epi)genetic mechanisms and future therapies of Hirschsprung disease — a review

2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Emilie G. Jaroy ◽  
Lourdes Acosta-Jimenez ◽  
Ryo Hotta ◽  
Allan M. Goldstein ◽  
Ragnhild Emblem ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nervous System ◽  
Enteric Nervous System ◽  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Current Knowledge ◽  
Hirschsprung Disease ◽  
Gut Development ◽  
Dna And Rna ◽  
Expression Of Genes

Abstract Hirschsprung disease is a neurocristopathy, characterized by aganglionosis in the distal bowel. It is caused by failure of the enteric nervous system progenitors to migrate, proliferate, and differentiate in the gut. Development of an enteric nervous system is a tightly regulated process. Both the neural crest cells and the surrounding environment are regulated by different genes, signaling pathways, and morphogens. For this process to be successful, the timing of gene expression is crucial. Hence, alterations in expression of genes specific for the enteric nervous system may contribute to the pathogenesis of Hirschsprung’s disease. Several epigenetic mechanisms contribute to regulate gene expression, such as modifications of DNA and RNA, histone modifications, and microRNAs. Here, we review the current knowledge of epigenetic and epitranscriptomic regulation in the development of the enteric nervous system and its potential significance for the pathogenesis of Hirschsprung’s disease. We also discuss possible future therapies and how targeting epigenetic and epitranscriptomic mechanisms may open new avenues for novel treatment.

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