scholarly journals Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast

2020 ◽  
Author(s):  
William T. Molin ◽  
Allison Yaguchi ◽  
Mark Blenner ◽  
Christopher Saski
Keyword(s):  
Sequence Analysis ◽  
Dna Unwinding ◽  
Amaranthus Palmeri ◽  
Yeast Plasmid ◽  
Autonomous Replication ◽  
Close Proximity ◽  
Self Replication ◽  
Autonomous Replication Sequence

Abstract Objective: The objective of the research presented here was to determine whether autonomous replication sequences (ARS) discovered in the eccDNA replicon of glyphosate resistant Amaranthus palmeri enable self-replication in a yeast system. Results: Sequence analysis of the eccDNA replicon revealed a region of sharp changes in A+T/G+C content with characteristic bending indicative of an autonomous replication sequence. Further sequence analysis revealed an extended autonomous replication sequence (EACS) in close proximity to multiple DNA unwinding element (DUE) sequences. This region of the eccDNA replicon enabled autonomous replication of an ARS-less yeast plasmid.

scholarly journals Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast

2020 ◽  
Author(s):  
William T. Molin ◽  
Allison Yaguchi ◽  
Mark Blenner ◽  
Christopher Saski
Keyword(s):  
Sequence Analysis ◽  
Dna Unwinding ◽  
Amaranthus Palmeri ◽  
Yeast Plasmid ◽  
Autonomous Replication ◽  
Close Proximity ◽  
Self Replication ◽  
Autonomous Replication Sequence

Abstract Objective The objective of the research presented here was to determine whether autonomous replication sequences (ARS) discovered in the eccDNA replicon of glyphosate resistant Amaranthus palmeri enable self-replication in a yeast system. Results Sequence analysis of the eccDNA replicon revealed a region of sharp changes in A + T/G + C content with characteristic bending indicative of an autonomous replication sequence. Further sequence analysis revealed an extended autonomous replication sequence (EACS) in close proximity to multiple DNA unwinding element (DUE) sequences. This region of the eccDNA replicon enabled autonomous replication of an ARS-less yeast plasmid.

Cloning an Autonomous Replication Sequence fromAspergillus oryzae

1993 ◽  
Vol 45 (6) ◽  
pp. 209-211 ◽  
Author(s):  
K. Kudo ◽  
A. Okoshi ◽  
R. Usami ◽  
K. Horikoshi
Keyword(s):  
Autonomous Replication ◽  
Autonomous Replication Sequence

scholarly journals Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
William T. Molin ◽  
Allison Yaguchi ◽  
Mark Blenner ◽  
Christopher A. Saski
Keyword(s):  
Amaranthus Palmeri ◽  
Autonomous Replication

scholarly journals Cloning and characterization of an autonomous replication sequence from Coxiella burnetii.

1994 ◽  
Vol 176 (17) ◽  
pp. 5233-5243 ◽  
Author(s):  
M Suhan ◽  
S Y Chen ◽  
H A Thompson ◽  
T A Hoover ◽  
A Hill ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Autonomous Replication ◽  
Autonomous Replication Sequence

scholarly journals The selfish yeast plasmid uses the nuclear motor Kip1p but not Cin8p for its localization and equal segregation

2009 ◽  
Vol 185 (2) ◽  
pp. 251-264 ◽  
Author(s):  
Hong Cui ◽  
Santanu K. Ghosh ◽  
Makkuni Jayaram
Keyword(s):  
Chromosome Segregation ◽  
Mitotic Spindle ◽  
Spindle Pole Body ◽  
Spindle Pole ◽  
Reporter Plasmid ◽  
Yeast Plasmid ◽  
Pole Body ◽  
Close Proximity ◽  
Plasmid Partitioning ◽  
2 Micron Plasmid

The 2 micron plasmid of Saccharomyces cerevisiae uses the Kip1 motor, but not the functionally redundant Cin8 motor, for its precise nuclear localization and equal segregation. The timing and lifetime of Kip1p association with the plasmid partitioning locus STB are consistent with Kip1p being an authentic component of the plasmid partitioning complex. Kip1–STB association is not blocked by disassembling the mitotic spindle. Lack of Kip1p disrupts recruitment of the cohesin complex at STB and cohesion of replicated plasmid molecules. Colocalization of a 2 micron reporter plasmid with Kip1p in close proximity to the spindle pole body is reminiscent of that of a CEN reporter plasmid. Absence of Kip1p displaces the plasmid from this nuclear address, where it has the potential to tether to a chromosome or poach chromosome segregation factors. Exploiting Kip1p, which is subsidiary to Cin8p for chromosome segregation, to direct itself to a “partitioning center” represents yet another facet of the benign parasitism of the yeast plasmid.

scholarly journals Virologic Escape during Danoprevir (ITMN-191/RG7227) Monotherapy Is Hepatitis C Virus Subtype Dependent and Associated with R155K Substitution

2011 ◽  
Vol 56 (1) ◽  
pp. 271-279 ◽  
Author(s):  
Sharlene R. Lim ◽  
Xiaoli Qin ◽  
Simone Susser ◽  
John B. Nicholas ◽  
Christian Lange ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Sequence Analysis ◽  
Drug Susceptibility ◽  
Population Based ◽  
Drug Susceptibility Testing ◽  
Viral Escape ◽  
Hcv Rna ◽  
Close Proximity ◽  
Chronic Hcv

ABSTRACTDanoprevir is a hepatitis C virus (HCV) NS3/4A protease inhibitor that promotes multi-log10reductions in HCV RNA when administered as a 14-day monotherapy to patients with genotype 1 chronic HCV. Of these patients, 14/37 experienced a continuous decline in HCV RNA, 13/37 a plateau, and 10/37 a rebound. The rebound and continuous-decline groups experienced similar median declines in HCV RNA through day 7, but their results diverged notably at day 14. Plateau group patients experienced a lesser, but sustained, median HCV RNA decline. Baseline danoprevir susceptibility was similar across response groups but was reduced significantly at day 14 in the rebound group. Viral rebound in genotype 1b was uncommon (found in 2/23 patients). Population-based sequence analysis of NS3 and NS4A identified treatment-emergent substitutions at four amino acid positions in the protease domain of NS3 (positions 71, 155, 168, and 170), but only two (155 and 168) were in close proximity to the danoprevir binding site and carried substitutions that impacted danoprevir potency. R155K was the predominant route to reduced danoprevir susceptibility and was observed in virus isolated from all 10 rebound, 2/13 plateau, and 1/14 continuous-decline patients. Virus in one rebound patient additionally carried partial R155Q and D168E substitutions. Treatment-emergent substitutions in plateau patients were less frequently observed and more variable. Single-rebound patients carried virus with R155Q, D168V, or D168T. Clonal sequence analysis and drug susceptibility testing indicated that only a single patient displayed multiple resistance pathways. These data indicate the ascendant importance of R155K for viral escape during danoprevir treatment and may have implications for the clinical use of this agent.

Effects of Vincristine on Endothelial Microtubules in the Spinal Cord

1976 ◽  
Vol 34 ◽  
pp. 58-59
Author(s):  
John L. Beggs ◽  
John D. Waggener ◽  
Wanda Miller
Keyword(s):  
Spinal Cord ◽  
Biological Activity ◽  
Horseradish Peroxidase ◽  
Transport Mechanism ◽  
Vasogenic Edema ◽  
Vesicular Transport ◽  
Close Proximity

Microtubules (MT) are versatile organelles participating in a wide variety of biological activity. MT involvement in the movement and transport of cytoplasmic components has been well documented. In the course of our study on trauma-induced vasogenic edema in the spinal cord we have concluded that endothelial vesicles contribute to the edema process. Using horseradish peroxidase as a vascular tracer, labeled endothelial vesicles were present in all situations expected if a vesicular transport mechanism was in operation. Frequently,labeled vesicles coalesced to form channels that appeared to traverse the endothelium. The presence of MT in close proximity to labeled vesicles sugg ested that MT may play a role in vesicular activity.

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