scholarly journals Quantitative visualization of photosynthetic pigments in tea leaves based on Raman spectroscopy and calibration model transfer

2020 ◽  
Author(s):  
Jianjun Zeng ◽  
Wen Ping ◽  
Alireza Sanaeifar ◽  
Xiao Xu ◽  
Wei Luo ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Photosynthetic Pigments ◽  
Spectral Model ◽  
Calibration Model ◽  
Tea Leaves ◽  
Raman Spectrometer ◽  
Quantitative Visualization ◽  
Model Transfer ◽  
Portable Raman

Abstract Background: Photosynthetic pigments participating in the absorption, transformation and transfer of light energy play a very important role in plant growth. While, the spatial distribution of foliar pigments is an important indicator of environmental stress, such as pests, diseases and heavy metal stress. Results: In this paper, in situ quantitative visualization of chlorophyll and carotenoid was realized by combining the Raman spectroscopy with calibration model transfer, and a laboratory Raman spectral model was successfully extended to a portable field spectral measurement. Firstly, a nondestructive and fast model for determination of chlorophyll and carotenoid in tea leaf was established based on confocal micro-Raman spectrometer in the laboratory. Then the spectral model was extended to a real-time foliar map scanning spectra of a field portable Raman spectrometer through calibration model transfer, and the spectral variation between the confocal micro-Raman spectrometer in the laboratory and the portable Raman spectrometer were effectively corrected by the direct standardization (DS) algorithm. The portable map scanning Raman spectra of the tea leaves after the model transfer were got into the established quantitative determination model to predict the concentration of photosynthetic pigments at each pixel of the tea leaves. The predicted photosynthetic pigments concentration of each pixel was imaged to illustrate the distribution map of foliar pigments. Statistical analysis showed that the predicted pigment contents were highly correlated with the real contents.Conclusions: It can be concluded that the Raman spectroscopy was applicable for in situ, non-destructive and rapid quantitative detecting and imaging of photosynthetic pigment concentration in tea leaves, and the spectral detection model established based on the laboratory Raman spectrometer can be applied to a portable field spectrometer for quantitatively imaging of the foliar pigments.

scholarly journals Quantitative visualization of photosynthetic pigments in tea leaves based on Raman spectroscopy and calibration model transfer

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Jianjun Zeng ◽  
Wen Ping ◽  
Alireza Sanaeifar ◽  
Xiao Xu ◽  
Wei Luo ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Photosynthetic Pigments ◽  
Spectral Model ◽  
Calibration Model ◽  
Tea Leaves ◽  
Raman Spectrometer ◽  
Quantitative Visualization ◽  
Model Transfer ◽  
Portable Raman

Abstract Background Photosynthetic pigments participating in the absorption, transformation and transfer of light energy play a very important role in plant growth. While, the spatial distribution of foliar pigments is an important indicator of environmental stress, such as pests, diseases and heavy metal stress. Results In this paper, in situ quantitative visualization of chlorophyll and carotenoid was realized by combining the Raman spectroscopy with calibration model transfer, and a laboratory Raman spectral model was successfully extended to a portable field spectral measurement. Firstly, a nondestructive and fast model for determination of chlorophyll and carotenoid in tea leaf was established based on confocal micro-Raman spectrometer in the laboratory. Then the spectral model was extended to a real-time foliar map scanning spectra of a field portable Raman spectrometer through calibration model transfer, and the spectral variation between the confocal micro-Raman spectrometer in the laboratory and the portable Raman spectrometer were effectively corrected by the direct standardization (DS) algorithm. The portable map scanning Raman spectra of the tea leaves after the model transfer were got into the established quantitative determination model to predict the concentration of photosynthetic pigments at each pixel of the tea leaves. The predicted photosynthetic pigments concentration of each pixel was imaged to illustrate the distribution map of foliar pigments. Statistical analysis showed that the predicted pigment contents were highly correlated with the real contents. Conclusions It can be concluded that the Raman spectroscopy was applicable for in situ, non-destructive and rapid quantitative detecting and imaging of photosynthetic pigment concentration in tea leaves, and the spectral detection model established based on the laboratory Raman spectrometer can be applied to a portable field spectrometer for quantitatively imaging of the foliar pigments.

scholarly journals Quantitative visualization of photosynthetic pigments in tea leaves based on Raman spectroscopy and calibration model transfer

2020 ◽  
Author(s):  
Jianjun Zeng ◽  
Shuai Zhang ◽  
Wei Luo ◽  
Junjing Sha ◽  
Yifeng Huang ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Photosynthetic Pigments ◽  
Spectral Model ◽  
Calibration Model ◽  
Tea Leaves ◽  
Raman Spectrometer ◽  
Quantitative Visualization ◽  
Model Transfer ◽  
Portable Raman

Abstract Background: Photosynthetic pigments participating in the absorption, transformation and transfer of light energy play a very important role in plant growth. While, the spatial distribution of foliar pigments is an important indicator of environmental stress, such as pests, diseases and heavy metal stress. Results: In this paper, in situ quantitative visualization of chlorophyll and carotenoid was realized by combining the Raman spectroscopy with calibration model transfer, and a laboratory Raman spectral model was successfully extended to a portable field spectral measurement. Firstly, a nondestructive and fast model for determination of chlorophyll and carotenoid in tea leaf was established based on confocal micro-Raman spectrometer in the laboratory. Then the spectral model was extended to a real-time foliar map scanning spectra of a field portable Raman spectrometer through calibration model transfer, and the spectral variation between the confocal micro-Raman spectrometer in the laboratory and the portable Raman spectrometer were effectively corrected by the direct standardization (DS) algorithm. The portable map scanning Raman spectra of the tea leaves after the model transfer were got into the established quantitative determination model to predict the concentration of photosynthetic pigments at each pixel of the tea leaves. The predicted photosynthetic pigments concentration of each pixel was imaged to illustrate the distribution map of foliar pigments. Statistical analysis showed that the predicted pigment contents were highly correlated with the real contents. Conclusions: It can be concluded that the Raman spectroscopy was applicable for in situ, non-destructive and rapid quantitative detecting and imaging of photosynthetic pigment concentration in tea leaves, and the spectral detection model established based on the laboratory Raman spectrometer can be applied to a portable field spectrometer for quantitatively imaging of the foliar pigments.

Diethyldithiocarbamate (DDTC) induced formation of positively charged silver nanoparticles for rapid in situ identification of inorganic explosives by surface enhanced Raman spectroscopy

RSC Advances ◽  
2016 ◽  
Vol 6 (57) ◽  
pp. 51823-51829 ◽  
Author(s):  
Juan Chen ◽  
Yu-e Shi ◽  
Min Zhang ◽  
Jinhua Zhan
Keyword(s):  
Raman Spectroscopy ◽  
Silver Nanoparticles ◽  
Surface Enhanced Raman Spectroscopy ◽  
Raman Spectrometer ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Positively Charged ◽  
Portable Raman

Diethyldithiocarbamate could induce the generation of positively charged silver nanoparticles for rapidin situdetection of the explosives with a portable Raman spectrometer.

Characterisation of a portable Raman spectrometer for in situ analysis of art objects

2014 ◽  
Vol 118 ◽  
pp. 294-301 ◽  
Author(s):  
Debbie Lauwers ◽  
Anna Garcia Hutado ◽  
Vinka Tanevska ◽  
Luc Moens ◽  
Danilo Bersani ◽  
...  
Keyword(s):  
In Situ Analysis ◽  
Raman Spectrometer ◽  
Art Objects ◽  
Portable Raman

Portable Raman spectroscopy for an in-situ monitoring the ripening of tomato ( Solanum lycopersicum ) fruits

2017 ◽  
Vol 180 ◽  
pp. 138-143 ◽  
Author(s):  
Josu Trebolazabala ◽  
Maite Maguregui ◽  
Héctor Morillas ◽  
Alberto de Diego ◽  
Juan Manuel Madariaga
Keyword(s):  
Raman Spectroscopy ◽  
Solanum Lycopersicum ◽  
In Situ Monitoring ◽  
Portable Raman Spectroscopy ◽  
Portable Raman

scholarly journals Detection of carotenoids of halophilic prokaryotes in solid inclusions inside laboratory-grown chloride and sulfate crystals using a portable Raman spectrometer: applications for Mars exploration

2019 ◽  
Vol 366 (20) ◽  
Author(s):  
Adam Culka ◽  
Filip Košek ◽  
Aharon Oren ◽  
Lily Mana ◽  
Jan Jehlička
Keyword(s):  
Raman Spectroscopy ◽  
Carotenoid Pigments ◽  
Crystalline Layer ◽  
Raman Spectrometer ◽  
Resonance Enhancement ◽  
Mineral Assemblages ◽  
Detection Of Biomarkers ◽  
Primary Crystals ◽  
532 Nm ◽  
Portable Raman

ABSTRACT Inclusions in evaporitic minerals sometimes contain remnants of microorganisms or biomarkers, which can be considered as traces of life. Raman spectroscopy with resonance enhancement is one of the best analytical methods to search for such biomarkers in places of interest for astrobiology, including the surface and near subsurface of planet Mars. Portable Raman spectrometers are used as training tools for detection of biomarkers. Investigations of the limits and challenges of detecting biomolecules in crystals using Raman spectroscopy is important because natural occurrences often involve mineral assemblages as well as their fluid and solid inclusions. A portable Raman spectrometer with 532 nm excitation was used for detection of carotenoid biomarkers: salinixanthin of Salinibacter ruber (Bacteroidetes) and α-bacterioruberin of Halorubrum sodomense (Halobacteria) in laboratory-grown artificial inclusions in compound crystals of several chlorides and sulfates, simulating entrapment of microorganisms in evaporitic minerals. Crystals of halite (NaCl), sylvite (KCl), arcanite (K2SO4) and tschermigite ((NH4)Al(SO4)2·12H2O) were grown from synthetic solutions that contained microorganisms. A second crystalline layer of NaCl or K2SO4 was grown subsequently so that primary crystals containing microorganisms are considered as solid inclusions. A portable Raman spectrometer with resonance enabling excitation detected signals of both carotenoid pigments. Correct positions of diagnostic Raman bands corresponding to the specific carotenoids were recorded.

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