scholarly journals Neonatal Tactile Stimulation Modulates Dendritic Spine Densities in Somatosensory Cortex of Adult WAG/Rij rats

2021 ◽  
Author(s):  
Gul Ilbay ◽  
Aymen Balikci ◽  
Sibel Kokturk ◽  
Melda Yardimoglu Yilmaz ◽  
Nurbay Ates
Keyword(s):  
Somatosensory Cortex ◽  
Dendritic Spine ◽  
Tactile Stimulation ◽  
Maternal Separation ◽  
Brain Structures ◽  
Absence Epilepsy ◽  
Absence Seizure ◽  
Spine Density ◽  
Dendrite Structure ◽  
Dendrite Spine

Abstract Objective: The aim of our study is to examine the effects of neonatal tactile stimulatons on the brain structures that previously defined as the focus of epilepsy in the Wistar-Albino-Glaxo from Rijswijk (WAG/Rij) rat brain with genetic absence epilepsy.Methods: In the present research, morphology and density of dendritic spines were analyzed in the somatosensory cortex (SoCx) of WAG/Rij rats (non stimulated control, tactile-stimulated and maternal separated rats) and healthy Wistar (non-epileptic) rats. To achieve this, a Golgi-Cox method was used.Results: Dendritic spine number in layer V of the SoCx has been detected significantly higher in adult WAG/Rij rats at post natal day 150 in comparison to non-epileptic adult control Wistar rats (p<0,001). Moreover, quantitative analyses of dendrite structure in adult WAG/Rij rats showed a decrease in dendrite spine density of pyramidal neurons of SoCx which occurred in early neonatal exposure to maternal separation (MS) and tactile stimulation (TS) (p<0,001).Conclusions: Our findings provide the first evidence that tactile stimulations during the early postnatal period have a long-term impact on dendrite structure in WAG/Rij rat’s brain and suggest a reduction in dendrite spine density is linked to absence seizure reduction.

scholarly journals Neonatal Tactile Stimulation Modulates Dendritic Spine Densities in Somatosensory Cortex of Adult WAG/Rij rats

2020 ◽  
Author(s):  
Gul Ilbay ◽  
Aymen Balikci ◽  
Sibel Kokturk ◽  
Melda Yardimoglu Yilmaz ◽  
Nurbay Ates
Keyword(s):  
Somatosensory Cortex ◽  
Dendritic Spine ◽  
Tactile Stimulation ◽  
Maternal Separation ◽  
Brain Structures ◽  
Absence Epilepsy ◽  
Absence Seizure ◽  
Spine Density ◽  
Dendrite Structure ◽  
Dendrite Spine

Abstract Background In previous studies, it has been shown that in early developmental period, the high level of tactile stimulations prevent absence epilepsy development and comorbid depression in Wistar-Albino-Glaxo from Rijswijk (WAG/Rij) rats with absence epilepsy. The aim of our study is to examine the effects of neonatal tactile stimulatons on the brain structures that previously defined as the focus of epilepsy in the rat brain with genetic absence epilepsy. Results In the present research, morphology and density of dendritic spines were analyzed in the somatosensory cortex (SoCx) in WAG/Rij rats with absence epilepsy, sensory experienced WAG/Rij rats (tactile-stimulated and maternal separated) and healthy Wistar (non-epileptic) rats. To achieve this, a Golgi-Cox method was used. Dendritic spine number in layer V of the SoCx has been detected significantly higher in adult WAG/Rij rats at post natal day 150 in comparison to non-epileptic adult control Wistar rats (p < 0,001). Moreover, quantitative analyses of dendrite structure in adult WAG/Rij rats showed a decrease in dendrite spine density of pyramidal neurons of SoCx which occurred in early neonatal exposure to maternal separation (MS) and tactile stimulation (TS) (p < 0,001). Conclusions Our findings provide the first evidence that tactile stimulations during the early postnatal period have a long-term impact on dendrite structure in WAG/Rij rat’s brain and suggest a reduction in dendrite spine density is linked to absence seizure reduction.

scholarly journals Neonatal Tactile Stimulation Modulates Dendritic Spine Densities in Somatosensory Cortex of Adult WAG/Rij rats

2021 ◽  
Author(s):  
Gul Ilbay ◽  
Aymen Balikci ◽  
Sibel Kokturk ◽  
Melda Yardimoglu Yilmaz ◽  
Nurbay Ates
Keyword(s):  
Somatosensory Cortex ◽  
Dendritic Spine ◽  
Tactile Stimulation ◽  
Maternal Separation ◽  
Brain Structures ◽  
Absence Epilepsy ◽  
Absence Seizure ◽  
Spine Density ◽  
Dendrite Structure ◽  
Dendrite Spine

Abstract Objective: The aim of our study is to examine the effects of neonatal tactile stimulatons on the brain structures that previously defined as the focus of epilepsy in the Wistar-Albino-Glaxo from Rijswijk (WAG/Rij) rat brain with genetic absence epilepsy.Methods: In the present research, morphology and density of dendritic spines were analyzed in the somatosensory cortex (SoCx) of WAG/Rij rats (non stimulated control, tactile-stimulated and maternal separated rats) and healthy Wistar (non-epileptic) rats. To achieve this, a Golgi-Cox method was used.Results: Dendritic spine number in layer V of the SoCx has been detected significantly higher in adult WAG/Rij rats at post natal day 150 in comparison to non-epileptic adult control Wistar rats (p<0,001). Moreover, quantitative analyses of dendrite structure in adult WAG/Rij rats showed a decrease in dendrite spine density of pyramidal neurons of SoCx which occurred in early neonatal exposure to maternal separation (MS) and tactile stimulation (TS) (p<0,001).Conclusions: Our findings provide the first evidence that tactile stimulations during the early postnatal period have a long-term impact on dendrite structure in WAG/Rij rat’s brain and suggest a reduction in dendrite spine density is linked to absence seizure reduction.

scholarly journals FMRP regulates the subcellular distribution of cortical dendritic spine density in a non-cell-autonomous manner

2021 ◽  
pp. 105253
Author(s):  
Katherine M. Bland ◽  
Adam Aharon ◽  
Eden L. Widener ◽  
M. Irene Song ◽  
Zachary O. Casey ◽  
...  
Keyword(s):  
Dendritic Spine ◽  
Subcellular Distribution ◽  
Spine Density ◽  
Dendritic Spine Density

scholarly journals BDNF-TrkB controls cocaine-induced dendritic spines in rodent nucleus accumbens dissociated from increases in addictive behaviors

2017 ◽  
Vol 114 (35) ◽  
pp. 9469-9474 ◽  
Author(s):  
Ethan M. Anderson ◽  
Anne Marie Wissman ◽  
Joyce Chemplanikal ◽  
Nicole Buzin ◽  
Daniel Guzman ◽  
...  
Keyword(s):  
Nucleus Accumbens ◽  
Dendritic Spine ◽  
Morphological Changes ◽  
Dominant Negative ◽  
Addictive Behaviors ◽  
Spine Density ◽  
Nucleus Accumbens Shell ◽  
Self Administration ◽  
Dendritic Spine Density ◽  
Chronic Cocaine

Chronic cocaine use is associated with prominent morphological changes in nucleus accumbens shell (NACsh) neurons, including increases in dendritic spine density along with enhanced motivation for cocaine, but a functional relationship between these morphological and behavioral phenomena has not been shown. Here we show that brain-derived neurotrophic factor (BDNF) signaling through tyrosine kinase B (TrkB) receptors in NACsh neurons is necessary for cocaine-induced dendritic spine formation by using either localized TrkB knockout or viral-mediated expression of a dominant negative, kinase-dead TrkB mutant. Interestingly, augmenting wild-type TrkB expression after chronic cocaine self-administration reverses the sustained increase in dendritic spine density, an effect mediated by TrkB signaling pathways that converge on extracellular regulated kinase. Loss of TrkB function after cocaine self-administration, however, leaves spine density intact but markedly enhances the motivation for cocaine, an effect mediated by specific loss of TrkB signaling through phospholipase Cgamma1 (PLCγ1). Conversely, overexpression of PLCγ1 both reduces the motivation for cocaine and reverses dendritic spine density, suggesting a potential target for the treatment of addiction in chronic users. Together, these findings indicate that BDNF-TrkB signaling both mediates and reverses cocaine-induced increases in dendritic spine density in NACsh neurons, and these morphological changes are entirely dissociable from changes in addictive behavior.

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