scholarly journals Transcriptome Analysis Based on Lr19-Virulent Mutants Strain Provides Clues for the Pathogenicity-Related Genes and Effectors of Puccinia Triticina

2020 ◽  
Author(s):  
Wenyue Wu ◽  
Fan Fan ◽  
Fei Wang ◽  
Zhongchi Cui ◽  
Xinkang Sun ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Rapid Evolution ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Differentially Expressed ◽  
Leaf Rust Resistance Gene ◽  
Post Inoculation ◽  
Wheat Leaf Rust ◽  
Wheat Leaf ◽  
Race 1

Abstract Background: Wheat leaf rust caused by Puccinia triticina (Pt) remains one of the most destructive diseases of common wheat worldwide. Cultivating resistant cultivars is an effective way to control this disease, but race-specific resistance can be overcome quickly due to the rapid evolution of Pt populations. The critical to control wheat leaf rust is to understand the pathogenicity mechanisms of Pt. Results: In this study, the spores of the Pt race PHNT (wheat leaf rust resistance gene Lr19-avirulent isolate) were mutagenized with ethyl methanesulfonate (EMS) and two Pt Lr19-virulent mutants named M1 and M2 were isolated, suggesting that they carry mutations affecting the Lr19-specific avirulent factor. RNA sequencing was performed on samples collected from the wheat cultivars Chinese Spring and TcLr19 that were infected by wild-type (WT) PHNT and the two Lr19-virulent mutant isolates at 14 days post-inoculation (dpi). The assembled transcriptome data were compared to the reference genome “Pt 1-1 BBBD Race 1.” A total of 216 differentially expressed genes (DEGs) were found from three different sample comparisons including M1-vs-WT, M2-vs-WT, and M1-vs-M2. Of these DEGs, 29 common DEGs were shared between M1-vs-WT and M2-vs-WT comparisons. Among the 216 DEGs encoding proteins, 30 were predicted to be effector candidates. Then 6 effector candidates (PTTG_27844, PTTG_05290, PTTG_27401, PTTG_27224, PTTG_26282, PTTG_25521) were verified that these genes were differentially expressed during Pt infection by quantitative real-time PCR (qRT-PCR) and were validated on tobacco, and the results showed that PTTG_27401 could inhibit progress of cell death (PCD) induced by BAX.Conclusions: Our results showed that a large number of genes participate in the interaction between Pt and TcLr19, which will provide valuable resources for the identification and targeting of AvrLr19 effector candidates and pathogenesis-related genes. Furthermore, our analyses are of great significance to reveal the pathogenesis of Pt.

scholarly journals Highly predictive SNP markers for efficient selection of the wheat leaf rust resistance gene Lr16

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Mulualem T. Kassa ◽  
Frank M. You ◽  
Colin W. Hiebert ◽  
Curtis J. Pozniak ◽  
Pierre R. Fobert ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Snp Markers ◽  
Leaf Rust Resistance Gene ◽  
Wheat Leaf Rust ◽  
Wheat Leaf ◽  
Efficient Selection ◽  
Selection Of

Virulence and molecular polymorphisms of the wheat leaf rust fungus Puccinia triticina in Canada from 1997 to 2007

Botany ◽  
2010 ◽  
Vol 88 (6) ◽  
pp. 575-589 ◽  
Author(s):  
Xiben Wang ◽  
Guus Bakkeren ◽  
Brent McCallum
Keyword(s):  
Leaf Rust ◽  
Expressed Sequence Tag ◽  
Rust Fungus ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Significant Shift ◽  
Wheat Leaf Rust ◽  
Rust Disease ◽  
The Pacific ◽  
Wheat Leaf

Populations of Puccinia triticina , one of the casual agents of wheat leaf rust disease, in the pacific (British Columbia and Alberta), prairie (Manitoba and Saskatchewan), and eastern regions (Quebec and Ontario) of Canada from 1997 to 2007 were analyzed for virulence and genetic diversity by revealing expressed sequence tag derived simple sequence repeat (EST-SSR) polymorphisms. Since 1997, a significant shift in the virulence of P. triticina occurred across Canada. The diversity of P. triticina virulence phenotypes in Manitoba and Saskatchewan, as measured by Shannon and Simpson indexes, decreased due to the directional selection toward predominant virulence phenotypes, whereas it remained relatively constant in Quebec and Ontario. The clustering of P. triticina virulence phenotypes from 1997 to 2007 was similar to that found in previous years, and was correlated with virulence to leaf rust resistance genes Lr2a, Lr2c, and Lr17a. Distinct EST-SSR profiles were found in different groups of P. triticina virulence phenotypes based on virulence to Lr2a, Lr2c, and Lr17a. In addition, the population of P. triticina in Manitoba and Saskatchewan was different from that in Quebec and Ontario from 1997 to 2007, based on both virulence characteristics and EST-SSR genotypes.

An interchromosomal reciprocal translocation in wheat involving leaf rust resistance gene Lr34

Genome ◽  
1994 ◽  
Vol 37 (4) ◽  
pp. 556-559 ◽  
Author(s):  
P. L. Dyck ◽  
E. R. Kerber ◽  
T. Aung
Keyword(s):  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Adult Plant ◽  
Leaf Rust Resistance Gene ◽  
Wheat Leaf Rust ◽  
Wheat Leaf ◽  
Mother Cells

'Thatcher' backcross lines RL6058 and RL6077 have adult-plant leaf rust resistance and were believed to have Lr34. However, genetic analysis revealed that the genes in the two lines were independent of each other. Previous work demonstrated that Lr34 is located on chromosome 7D. The leaf rust resistance gene in RL6058 must be on chromosome 7DS because no recombinants were observed between it and gene Lr29, known to be on chromosome 7DS. It was also linked with Rc3 (30.25 ± 2.88%), a gene for purple coleoptile on chromosome 7DS. It was independent of Lr19 and NS1 (nonsuppressor mutant), which are located on 7DL. The leaf rust resistance gene in RL6077 was independent of genes Lr19 and Lr29. The presence of quadrivalents in pollen mother cells of the RL6058/RL6077 hybrid indicates that the Lr34 gene in RL6077 may have been translocated onto another chromosome. Lr34 from RL6058 and RL6077 may have been combined in four F3 lines derived from their intercross.Key words: Triticum aestivum, wheat, leaf rust resistance.

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