scholarly journals Genetic Stability in Melon Transformation System: Assessment by Flow Cytometry and ISSR Markers

2021 ◽  
Author(s):  
Mohammad Reza Raji ◽  
Mostafa Farajpour
Keyword(s):  
Flow Cytometry ◽  
Genetic Stability ◽  
Genetic Instability ◽  
Integrated Approach ◽  
Polymerase Chain Reaction Analysis ◽  
Issr Markers ◽  
Selective Medium ◽  
Neomycin Phosphotransferase ◽  
Cotyledonary Explants

Abstract Genetic instability in melon species sometimes occurs as a result of in vitro tissue culture and transformation systems. This study describes a new regeneration technique for agrobacterium-mediated co-culture of muskmelon explants (Cucumis melo L. c.v. ‘Khatooni’). Here, no genetic instability was observed in positive PCR regenerants. 4-day-old cotyledonary explants had been infected with LBA4404 Agrobacterium suspensions. The co-cultivation occurred in the presence of 100mg/l rifampicin and 50mg/l kanamycin. The bacteria contained a binary vector pBI121 carrying nopaline synthase by the promoter-neomycin phosphotransferase gene. The regeneration succeeded 65% in selective MS medium containing N6-benzylaminopurine (600 µg/l), β-naphthoxyacetic acid (25 µg/l) and 50mg/l kanamycin in inoculated 4-day-old cotyledonary explants. According to the polymerase chain reaction analysis of neomycin phosphotransferase II gene, transformation was merely successful (8.4%), indicating a substantial miss on a large number of regenerated plants in the selective medium, as a consequence of PGR and antibiotic imbalances. Inter Single Sequence Repeat markers and flow cytometry analyses were used for evaluating the genetic stability and ploidy level of transplants, respectively. The integrated approach underlined that Agrobacterium inoculation and plant growth regulators were successfully combined in vitro to enable muskmelon transformation.

scholarly journals ASSESSMENT OF GENETIC STABILITY OF MICROPROPAGATED Eucalyptus globulus Labill HYBRID CLONES BY MEANS OF FLOW CYTOMETRY AND MICROSATELLITES MARKERS

2017 ◽  
Vol 41 (1) ◽  
Author(s):  
Leandro Silva Oliveira ◽  
Aloisio Xavier ◽  
Wagner Campos Otoni ◽  
José Marcello Salabert Campos ◽  
Lyderson Facio Viccini ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Microsatellite Markers ◽  
Genetic Stability ◽  
Culture Medium ◽  
Eucalyptus Grandis ◽  
Genetic Fidelity ◽  
Shoot Multiplication ◽  
Micropropagated Plants ◽  
Hybrid Clones

ABSTRACT Flow cytometry and microsatellite markers were used to determine a genetic fidelity of micropropagated plants from the two Eucalyptus urophylla x E. globulus clones and a Eucalyptus grandis x E. globulus clone derived from adult material. Clones were repeatedly subcultured for 25 subcultures on MS medium supplemented with BA (2.22 µM) and ANA (0.05 µM) for in vitro shoot multiplication. The elongation was performed in MS culture medium supplemented with AIB (2.46 µM) and BA(0.22 µM). The ex vitro rooting and acclimatization phases were lead at the same time. The micropropagated clones showed genetic stability by flow cytometry and microsatellite markers. The results proved that micropropagation, for purposes of rejuvenation, can be a viable technique to generate genetically stable or identical E. globulus hybrid clones.

Thidiazuron induced in vitro multiplication of Mentha arvensis and evaluation of genetic stability by flow cytometry and molecular markers

2014 ◽  
Vol 62 ◽  
pp. 100-106 ◽  
Author(s):  
Mohammad Faisal ◽  
Abdulrahman A. Alatar ◽  
Ahmad K. Hegazy ◽  
Sulaiman A. Alharbi ◽  
Mohammad El-Sheikh ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Molecular Markers ◽  
Genetic Stability ◽  
Mentha Arvensis ◽  
In Vitro Multiplication

Genetic stability using RAPD and ISSR markers in efficiently in vitro regenerated plants of Inula royleana DC

Meta Gene ◽  
2018 ◽  
Vol 18 ◽  
pp. 100-106 ◽  
Author(s):  
Samar Amin ◽  
Tareq A. Wani ◽  
Zahoor Ahmad Kaloo ◽  
Seema Singh ◽  
Riffat John ◽  
...  
Keyword(s):  
Genetic Stability ◽  
Issr Markers ◽  
Regenerated Plants ◽  
Rapd And Issr

Assessment of Genetic Stability in Three Generations of In Vitro Propagated Jatropha curcas L. Plantlets Using ISSR Markers

2016 ◽  
Vol 9 (4) ◽  
pp. 229-238 ◽  
Author(s):  
Douglas Moraes Mendel Soares ◽  
Mariana Cansian Sattler ◽  
Marcia Flores da Silva Ferreira ◽  
Milene Miranda Praça-Fontes
Keyword(s):  
Jatropha Curcas ◽  
Genetic Stability ◽  
Issr Markers ◽  
Jatropha Curcas L ◽  
Three Generations

scholarly journals Assessment of Genetic Stability on In Vitro And Ex Vitro Plants of Ficus Carica Var. Black Jack Using Issr and Damd Markers

2021 ◽  
Author(s):  
Ankita Rajendra Parab ◽  
Chew Bee Lynn ◽  
Sreeramanan Subramaniam
Keyword(s):  
Stability Analysis ◽  
Genetic Stability ◽  
Similarity Index ◽  
Issr Markers ◽  
Ficus Carica ◽  
Ex Vitro ◽  
Regenerated Plants ◽  
Inter Simple Sequence Repeats ◽  
Dna Primers

Abstract In vitro propagation has been significant in producing a large number of genetically stable regenerated plants. Regenerated Ficus carica var. Black Jack plantlets were established using woody plant medium (WPM) supplemented with 20 µM 6-Benzylaminopurine (BAP) and 8 µM Indole-3-acetic acid (IAA) under different light treatments such as normal fluorescent white light (60 µmol.m− 2.s− 1), and four different LED spectra, white (400– 700nm), blue (440nm), red (660nm) and blue + red (440nm + 660nm). Genetic stability analysis was performed on the in vitro and ex vitro plants of Ficus carica var. Black Jack. Ten (10) primers of each ISSR and DAMD molecular marker were used to assess the genetic stability of the eight (8) samples of Ficus carica var. Black Jack, acquired over two years. The findings of this study revealed that inter simple sequence repeats (ISSR) and directed amplification of minisatellite DNA (DAMD) markers (DNA primers) are efficient in determining the polymorphism and monomorphism percentage among the in vitro and ex vitro samples of Ficus carica var. Black Jack. ISSR markers showed 97.87% of monomorphism whereas DAMD markers showed 100% monomorphism. Polymorphism of 2.13% was observed for the UBC840 ISSR – DNA primer which was negated under the genetic similarity index analysis for the eight samples. It is recommended that genetic stability analysis should be performed for long-term maintenance of micropropagated plants.

scholarly journals Genetic Stability of In vitro Multiplied Phalaenopsis gigantea Protocorm-like Bodies as Affected by Chitosan

2013 ◽  
Vol 41 (1) ◽  
pp. 177 ◽  
Author(s):  
Samira SAMARFARD ◽  
Mihdzar A. KADIR ◽  
Saleh B. KADZIMIN ◽  
Seyedali RAVANFAR ◽  
Halimi M. SAUD
Keyword(s):  
Plant Growth ◽  
Plant Species ◽  
Genetic Stability ◽  
Liquid Culture ◽  
Issr Markers ◽  
Mother Plant ◽  
Liquid Media ◽  
Carbohydrate Polymer ◽  
Media Types

Chitosan is a carbohydrate polymer derivative of chitin which presents in shell of crustaceans. This biopolymer is a non toxic and environmentally friendly, considered as a plant growth stimulator in some plant species. The present study investigates the effects of chitosan and media types on multiplication and genetic stability of Phalaenopsis gigantea protocorm-like bodies (PLBs). PLBs were inoculated in liquid New Dogashima Medium (NDM) and Vacin and Went (VW) supplemented with various concentrations of chitosan (0, 5, 10, 15, 20 and 25 mg/L). The highest PLB multiplication was observed on VW and NDM supplemented with 10 mg/L chitosan with mean number of PLBs 177 and 147, respectively. Chitosan promoted the formation of juvenile leaves and the highest number was observed in NDM supplemented with 20 mg/L chitosan with mean number of 66 leaves after 8 weeks of culture. Genetic stability was assessed among mother plant and secondary PLBs after 2, 4, 6, and 8 weeks of culture in liquid media. 8 out of 10 ISSR markers produced a total of 275 clear and reproducible bands with mean of 6.9 bands per primer. The secondary PLBs produced during sub-culturing process of chitosan treated liquid culture were genetically uniform and similar to mother plant.

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