Persistence of parental age effect on somatic mutation rates across generations in Arabidopsis.

In the model plant Arabidopsis thaliana, parental age is known to affect somatic mutation rates in their immediate progeny but it is not clear if this age-associated effect on mutation rates persist across successive generations. Using a set of detector lines carrying the mutated uidA gene, we examined if a particular parental age maintained across five consecutive generations affected the rates of base substitution (BSR), intrachromosomal recombination (ICR), frameshift mutation (FS), and transposition. The frequency of functional GUS (blue colored spots) reversions were examined in seedlings as a function of identical/different parental ages across generations. When parental age remained constant, no change was observed in BSR/ICR rates in the first three generations, following which it drops significantly in the 4th and in most instances, is elevated in the 5th generation. On the other hand, with advancing parental age, BSR/ICR rates respectively remained high in the first two/three generations with a striking resemblance in the pattern of mutation rates. We followed a novel approach of identifying and tagging flowers pollinated on a particular day, thereby avoiding possible emasculation induced stress responses, as it may influence mutation rates. By and large there is no correlation in the expression of candidate genes involved in DNA repair to the pattern of reversion events and possibly, the expression patterns may correspond to the genomewide somatic mutations rates. Our results suggest a time component in counting the number of generations a plant has passed through self-fertilization at a particular age in determining the somatic mutation rates.

Two Hits of EDCs Three Generations Apart: Effects on Social Behaviors in Rats, and Analysis by Machine Learning

All individuals are directly exposed to extant environmental endocrine-disrupting chemicals (EDCs), and indirectly exposed through transgenerational inheritance from our ancestors. Although direct and ancestral exposures can each lead to deficits in behaviors, their interactions are not known. Here we focused on social behaviors based on evidence of their vulnerability to direct or ancestral exposures, together with their importance in reproduction and survival of a species. Using a novel “two hits, three generations apart” experimental rat model, we investigated interactions of two classes of EDCs across six generations. PCBs (a weakly estrogenic mixture Aroclor 1221, 1 mg/kg), Vinclozolin (antiandrogenic, 1 mg/kg) or vehicle (6% DMSO in sesame oil) were administered to pregnant rat dams (F0) to directly expose the F1 generation, with subsequent breeding through paternal or maternal lines. A second EDC hit was given to F3 dams, thereby exposing the F4 generation, with breeding through the F6 generation. Approximately 1200 male and female rats from F1, F3, F4 and F6 generations were run through tests of sociability and social novelty as indices of social preference. We leveraged machine learning using DeepLabCut to analyze nuanced social behaviors such as nose touching with accuracy similar to a human scorer. Surprisingly, social behaviors were affected in ancestrally exposed but not directly exposed individuals, particularly females from a paternally exposed breeding lineage. Effects varied by EDC: Vinclozolin affected aspects of behavior in the F3 generation while PCBs affected both the F3 and F6 generations. Taken together, our data suggest that specific aspects of behavior are particularly vulnerable to heritable ancestral exposure of EDC contamination, that there are sex differences, and that lineage is a key factor in transgenerational outcomes.

On planning, planning theories, and practices: A critical reflection

The futility of defining planning suggests that there is no planning as a recognizable practice. Sociology of knowledge definitions imply three kinds of planning practices: (1) Generic “planning”—what people do when they are planning; (2) Knowledge-centered “something” (e.g., spatial) planning; and (3) Real planning practiced in specific contexts, from metro-regional planning for Jakarta to transportation planning for the Trans-Europe Network, and enacted in general contexts, for example, informal- or Southern planning. Planning theories are linked to different practices: generic “planning” theories and “something” (e.g., regional, community, environmental, or Southern) planning theories. Selected topics illustrate the “planning” theory discourse and spatial planning theories are briefly reviewed. Three generations of planning practice studies are reviewed: the first, a-theoretical; the second, the “practice movement,” who studied practice for their own theorizing; and the third, informed by practice theories. Five books about planning show how their planning theorist authors understand planning practice. While recognizing planning as diverse practices, they hardly apply “planning” theory to planning practices. “Planning” theories are divorced from enacted planning practices, “something” (e.g., spatial) planning theories include constructive adaptations of “planning” theories and paradigms, but knowledge about real planning practices is limited. Implications from these conclusions are drawn for planning theory, education, and practices.

Dwujęzyczność polsko-niemiecka w powiecie pilskim, czarnkowsko-trzcianeckim i złotowskim: zarządzanie językiem w trzech pokoleniach na podstawie reprezentatywnych biografii językowych

Polish-German Bilingualism in the Piła, Czarnków-Trzcianka and Złotów Districts: A Study of Language Management in Three Generations Based on Representative Language BiographiesAs part of the project entitled “Language across generations: Contact induced change in morpho-syntax in German-Polish bilingual speech”, members of the research team conducted interviews in Polish and German with 124 bilingual persons in Poland and Germany, including 14 in the Piła, Czarnków-Trzcianka and Złotów districts. Before 1945, Piła and Złotów belonged to the German province of Pomerania, and when the borders were moved both towns became part of Poland. This brought a complete change of the social, geopolitical and language reality of the inhabitants of the region. In the People’s Republic of Poland, efforts were made to eliminate the German language. The situation changed again after the breakthrough of 1989, when the German minority in Poland was officially recognised. On the basis of three language biographies of people born in the 1920s, 1930s and 1950s, which are representative for the Piła-Złotów region, this study presents how the language ideology of linking one country with one language operated over three generations of speakers. It also considers language management at the micro level, taking into account the changes which occurred in 1945 and 1989. Dwujęzyczność polsko-niemiecka w powiecie pilskim, czarnkowsko-trzcianeckim i złotowskim: zarządzanie językiem w trzech pokoleniach na podstawie reprezentatywnych biografii językowychW ramach grantu „Pokoleniowe zróżnicowanie języka: zmiany morfosyntaktyczne wywołane przez polsko-niemiecki kontakt językowy w mowie osób dwujęzycznych” przeprowadzono wywiady ze stu dwudziestoma czterema osobami dwujęzycznymi w Polsce i w Niemczech w języku polskim i niemieckim, w tym w powiecie pilskim, czarnkowsko-trzcianeckim i złotowskim – z czternastoma osobami. Piła i Złotów należały przed 1945 rokiem do niemieckiej Prowincji Pomorza, a po przesunięciu granic oba miasta znalazły się w Polsce. Rzeczywistość społeczna, geopolityczna i językowa mieszkańców regionu całkowicie się zmieniła. W PRL dążono do wyeliminowania języka niemieckiego. Sytuacja ponownie uległa zmianie po przełomie 1989 roku, kiedy to uznano mniejszość niemiecką w Polsce. Na podstawie trzech biografii językowych osób urodzonych w latach 20., 30. i 50. XX wieku, reprezentatywnych dla regionu pilsko-złotowskiego, pokazano, jak na przestrzeni trzech pokoleń mówców funkcjonowała ideologia językowa łączenia jednego państwa z jednym językiem oraz przedstawiono zarządzanie językami na poziomie mikro z uwzględnieniem cezur czasowych 1945 i 1989 roku.

How Key Psychological Theories Can Enrich Our Understanding of Our Ancestors and Help Improve Mental Health for Present and Future Generations: A Family Historian’s Perspective

Family historians could increase their understanding of their ancestors and themselves and improve the mental health of living and future generations if they consider the psychological history of their forebears. Genealogists could then begin to recognize their family’s unique psychological inheritance that can appear as a result of trauma, depression, or addiction. The author explores three generations of a Parker family branch from Huntingdon/Norfolk, England, to show family historians how such considerations can shed light on their family’s psychological legacy. The author does this by introducing us to her great-grandmother Ann grandfather Walter, and mother Doreen through the lens of attachment theory, and their adverse childhood experiences (ACEs) such as poverty, bereavement, and addiction. Attachment matters because it affects not only how safe we feel, our ability to regulate our emotions and stress, our adaptability, resilience, and lifelong mental and physical health, but attachment style can also be passed on. In addition, this paper utilizes attachment theory to speculate on the likely attachment styles for the three generations of the Parker family and looks at the possible parenting behavior in the first two, the effect of alcoholism and the intergenerational impact of trauma and depression.

Life in Palestine Under the Rule of the Ottoman Empire According to Ibrahim Nassralla's Novel: Zaman al-Khoyoul al-Bayda'

This study deals with the Palestinian administrative, economic, political, educational, intellectual, and national dimensions as they are reflected in the stories and events of the historical novel Zaman al-Khoyoul al-Baida' by the Palestinian writer Ibrahim Nassralla, The novel that covers three generations from 1880s to the establishment of the State of Israel in 1948. The events take place in a Palestinian village called 'Hadiya ', which serves as a representative of all Palestine. The study proves that the writer emphasizes the Palestinian identity through the stories that he collected from people who lived through the three periods of occupation of Palestine: the Ottoman Empire, the British Mandate and Israel, but the main focus is on the Ottoman Period. Stylistically, the novel has a special printing style. The oral stories are typed in italics in order to distinguish them from written stories. To investigate the information in the people's quoted stories, the events of the novel and the writer's arguments and his descriptions of the life of local Palestinians, the study relies on Paul Hamilton's theory of historicism , which is a critical way of using historical contexts to interpret narrative texts.

Violence, History and Silence in Amitav Ghosh’s The Shadow Lines

This article analyzes Amitav Ghosh’s The Shadow Lines to examine a narrative gap at its heart that conceals the central fact of the death of Tridib, arguably the most important character in the text. The novel concentrates on the Partition of Bengal and its impact on people from different countries and nationalities in Asia and Europe. Accommodating the story of three generations of people in three cities--Dhaka, Calcutta, and London--The Shadow Lines shows the interaction of characters belonging to Hindu, Muslim, and Christian faith. Important events in the text revolve around the family of Mayadebi, her sister Tha’mma and the Prices, their English friends. The narrative begins in 1939 and ends in 1964, connecting the Second World War, the Partition in 1947, and the riots of 1964 in Calcutta and Dhaka. Using Tha’mma, the grandmother of the unnamed narrator, as the connecting link between their pre-modern life before Partition in Dhaka and diasporic life in post-Partition Calcutta, The Shadow Lines depicts the traumatic suffering of characters from different nationalities but mainly from India and Bangladesh. The paper argues that the silence and secrecy maintained by Tha’mma and others about Tridib’s death mirrors the silence of official history concerning violence in their narrative of civilization, freedom, and progress which Ghosh unravels to produce a novelistic revisionist history that not only challenges the mainstream history but also fills the gaps it leaves.

Van der woude syndrome with associated scaphocephaly: a rare case report

The purpose of this case is to provide a descriptive report of a rare combination of Van der Woude syndrome, recurrent in three generations of the same family, in a female patient with bilateral cleft lip and palate associated with a sagittal craniosynostosis (scaphocephaly).

Transgenerational Transmission of Trauma across Three Generations of Alevi Kurds

Background: Thus far, most researchers on genocide and transgenerational transmissions have focused on the National Socialist Holocaust as the most abhorrent example of this severe human rights violation. Few data have been published on other ethnic or religious groups affected by genocidal actions in this context. Methodology: Using a mixed-method approach integrating qualitative interviews with standardized instruments (SCID and PDS), this study examines how individual and collective trauma have been handed down across three generations in an Alevi Kurd community whose members (have) suffered genocidal perpetrations over a longer time period (a “genocidal environment”). Qualitative, open-ended interviews with members of three generations answering questions yielded information on (a) how their lives are shaped by the genocidal experiences from the previous generation and related victim experiences, (b) how the genocidal events were communicated in family narratives, and (c) coping strategies used. The first generation is the generation which directly suffered the genocidal actions. The second generation consists of children of those parents who survived the genocidal actions. Together with their family (children, partner, relatives), this generation suffered forced displacement. Members of the third generation were born in the diaspora where they also grew up. Results: Participants reported traumatic memories, presented in examples in this publication. The most severe traumatic memories included the Dersim massacre in 1937–1938 in Turkey, with 70,000–80,000 victims killed, and the enforced resettlement in western Turkey. A content analysis revealed that the transgenerational transmission of trauma continued across three generations. SCID and PDS data indicated high rates of distress in all generations. Conclusions: Genocidal environments such as that of the Kurdish Alevis lead to transgenerational transmission mediated by complex factors.