scholarly journals Soybean AP2-Domain Transcription Factor GmWRI4 Optimize Ratio of Oleic Acid to Linoleic Acid in Seed

2021 ◽  
Author(s):  
Qin Di ◽  
Angela Piersanti ◽  
Qi Zhang ◽  
Cristina Miceli ◽  
Xiaoyi Liu
Keyword(s):  
Transcription Factor ◽  
Linoleic Acid ◽  
Soybean Oil ◽  
Candidate Gene ◽  
Acid Content ◽  
Transcriptional Control ◽  
Natural Populations ◽  
Complex Mixture ◽  
Soybean Seed ◽  
Linoleic Acid Content

Abstract Background: Soybean [Glycine max (L.) Merrill] oil is a complex mixture of five fatty acids (oleic, linoleic, linolenic, palmitic and stearic). As a vegetable oils, soybean oil has a less than desirable fatty acid composition. The high content of linoleic acid contributes to poor oil oxidative stability. Soybean oil with lower linoleic acid content is desirable. To investigate the genetic architecture of linoleic acid in soybean seed, 510 soybean germplasms from China were collected as natural populations. Results: Phenotypic identification results showed that the content of linoleic acid varied from 36.22% to 72.18%. After a total of 2,423,512 nucleotide polymorphism (SNP) were obtained, a new candidate gene Glyma.04G116500.1 (GmWRI14) related to linoleic acid was discovered by 3-year long Genome-wide association analysis (GWAS). GmWRI14 belongs to the plant WRI1 protein family. The GmWRI14 showed a negative correlation with the linoleic acid content and the correlation coefficient was -0.912. To test whether GmWRI14 can lead to lower linoleic acid content in soybean seed, we introduced GmWRI14 into the soybean genome. Overexpression of GmWRI14 leads to higher accumulation of oil with lower linoleic acid content in soybean seed. RNA-seq verified that GmWRI14-overexpressed lines showed lower accumulation of GmFAD2-1 and GmFAD2-2b than non-transgenic lines. Conclusions: These results indicate that the down-regulation of FAD2 gene triggered by the transcription factor WRI1 is the underlying mechanism reducing linoleic acid level of seed oil. GmWRI14 is a new key candidate gene related to linoleic acid. These results will significantly improve understanding of the transcriptional control exerted by GmWRI14.

scholarly journals Increasing Conjugated Linoleic Acid Content in Milk and Cheese after Supplementing a Blend of Crude Soybean Oil Sediment Combined with Fish Oil to Grazing Dairy Cows

2020 ◽  
Vol 10 (03) ◽  
pp. 468-492
Author(s):  
Gerardo Antonio Gagliostro ◽  
Liliana Elisabet Antonacci ◽  
Carolina Daiana Pérez ◽  
Luciana Rossetti ◽  
Martín Tassone ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Fish Oil ◽  
Soybean Oil ◽  
Dairy Cows ◽  
Conjugated Linoleic Acid ◽  
Acid Content ◽  
Linoleic Acid Content ◽  
Crude Soybean Oil ◽  
Grazing Dairy Cows

Influence of Feeding Soybean Oil on Conjugated Linoleic Acid Content in Beef

2005 ◽  
Vol 53 (3) ◽  
pp. 684-689 ◽  
Author(s):  
Tilak R. Dhiman ◽  
Shahiduz Zaman ◽  
Kenneth C. Olson ◽  
Howard R. Bingham ◽  
Amy L. Ure ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Soybean Oil ◽  
Conjugated Linoleic Acid ◽  
Acid Content ◽  
Linoleic Acid Content

scholarly journals Milk Production, Conjugated Linoleic Acid Content, and In Vitro Ruminal Fermentation in Response to High Levels of Soybean Oil in Dairy Ewe Diet

2008 ◽  
Vol 91 (4) ◽  
pp. 1560-1569 ◽  
Author(s):  
P. Gómez-Cortés ◽  
P. Frutos ◽  
A.R. Mantecón ◽  
M. Juárez ◽  
M.A. de la Fuente ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Soybean Oil ◽  
Conjugated Linoleic Acid ◽  
Milk Production ◽  
Acid Content ◽  
Linoleic Acid Content ◽  
Ruminal Fermentation

scholarly journals Relationships Between Oleic and Linoleic Acid Content and Seed Colonization by Cercospora kikuchii and Diaporthe phaseolorum

Plant Disease ◽  
2008 ◽  
Vol 92 (7) ◽  
pp. 1038-1042 ◽  
Author(s):  
H. Q. Xue ◽  
R. G. Upchurch ◽  
P. Kwanyuen
Keyword(s):  
Linoleic Acid ◽  
Acid Content ◽  
Soybean Seed ◽  
Linoleic Acid Content ◽  
Oleic Acid Content ◽  
Cercospora Kikuchii ◽  
Diaporthe Phaseolorum ◽  
Soybean Genotypes ◽  
Seed Colonization

Compared with standard cultivars, seed of mid-oleic soybean genotypes sometimes have shown increased colonization by Cercospora kikuchii in the field as judged by increased levels of purple-stained seed. To examine relationships between oleic and linoleic acid levels in soybean seed and postharvest seed colonization by two fungal seed pathogens, we inoculated seed with differing oleic:linoleic acid (O/L) ratios. Seed with defined O/L ratios were produced by allowing seed development of two isogenic soybean lines to occur in three different air temperature environments. Seed produced in these environments were harvested, individually analyzed for fatty acid composition, and inoculated with mycelium preparations of the fungal seed pathogens C. kikuchii or Diaporthe phaseolorum var. sojae. Fungal biomass of infected seed was quantified by measuring in vitro ergosterol content. For both soybean lines, colonization by C. kikuchii was positively correlated with the O/L ratio (r = 0.55, P < 0.03) and oleic acid content (r = 0.61, P < 0.02), and negatively correlated with linoleic (r = –0.60, P < 0.02) and linolenic (r = –0.58, P < 0.03) acid content. No association was found between the extent of seed colonization by D. phaseolorum and the seed O/L ratio. Our data suggest that the O/L ratio may be related to soybean seed colonization by C. kikuchii, but there is no evidence of a relationship with D. phaseolorum var. sojae colonization.

Enhancement of linoleic acid content stimulates astaxanthin esterification in Coelastrum sp.

2020 ◽  
Vol 300 ◽  
pp. 122649 ◽  
Author(s):  
Zhiyong Liu ◽  
Yuyong Hou ◽  
Chunqing He ◽  
Xuan Wang ◽  
Shulin Chen ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Acid Content ◽  
Linoleic Acid Content

Laboratory Evaluation of Larvicidal and Oviposition Deterrent Properties of Edible Plant Oils for Potential Management of Aedes aegypti (Diptera: Culicidae) in Drinking Water Containers

2019 ◽  
Vol 56 (4) ◽  
pp. 1055-1063
Author(s):  
Teresia M Njoroge ◽  
May R Berenbaum
Keyword(s):  
Linoleic Acid ◽  
Aedes Aegypti ◽  
Acid Content ◽  
Edible Oils ◽  
Linoleic Acid Content ◽  
Laboratory Evaluation ◽  
Plant Oils ◽  
Edible Plant ◽  
Oviposition Deterrents ◽  
Olive Oils

Abstract The yellow fever mosquito, Aedes aegypti (Diptera: Culicidae) transmits several devastating arboviruses, including dengue, chikungunya, and Zika virus, making development of inexpensive and eco-friendly strategies for its control an urgent priority. We evaluated the lethality of 13 commonly used plant-derived edible oils against late-third instar Ae. aegypti and then tested the three most lethal oils for stage-specific differences in lethality. We also examined the effects of the most lethal (hempseed), moderately lethal (sunflower and peanut), and least lethal (olive) oils on survival to adulthood and oviposition behavior of gravid females. We hypothesized that the insecticidal activity of edible oils is a function of the content of their linoleic acid, a key fatty acid component with film-forming properties. Among the 13 oils tested, hempseed oil was the most lethal, with an LC50 of 348.25 ppm, followed by sesame (670.44 ppm) and pumpkinseed (826.91 ppm) oils. Oils with higher linoleic acid content were more lethal to larvae than those with low linoleic acid content. Furthermore, pure concentrated linoleic acid was more lethal to larvae compared to any edible oil. In comparison to early instars, late instars were more susceptible to hempseed, sunflower, peanut, and olive oils; these oils also acted as oviposition deterrents, with effective repellency ≥63%. The proportion of larvae surviving to adulthood was significantly reduced in hempseed, sunflower, peanut, and olive oil treatments relative to controls. Our results suggest that some edible plant oils have potential as effective, eco-friendly larvicides, and oviposition deterrents for controlling container-dwelling mosquitoes, especially in resource-limited settings.

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