Adventitious Shoot Regeneration and Plant Production from Explants of Apios americana

Shoots were regenerated from callus of Apios americana Medikus (apios, groundnut) using internodal explants from in vitro-germinated seedlings and from sprouted tubers on a modified Murashige and Skoog (MS) basal medium. Shoot regeneration was observed over a range of 2iP and IBA combinations. GA3 increased the number of shoots regenerated per epicotyl explant. The most efficient regeneration (≈90%) was with internodal epicotyl explants on 100 μm 2iP, 0.5 μm IBA, and 1.5 μm GA3. Regenerated shoots were rooted on liquid and solid MS medium with 0.5 μm IBA; however, rooting was more successful on the liquid medium. About 60% of rooted plants were successfully established in pots. Chemical names used: N-(3-methyl2-butenyl)-1 H-purin-6-amine (2iP), 1 H-indole-3-butanoic acid (IBA), gibberellic acid (G A3).

Download Full-text

Effects of Thidiazuron, basal medium and light quality on adventitious shoot regeneration from in vitro cultured stem of Populus alba×P. berolinensis

Journal of Forestry Research ◽

10.1007/s11676-008-0042-3 ◽

2008 ◽

Vol 19 (3) ◽

pp. 257-259 ◽

Cited By ~ 12

Author(s):

Hui-mei Wang ◽

Hong-mei Liu ◽

Wen-jie Wang ◽

Yuan-gang Zu

Keyword(s):

Shoot Regeneration ◽

Light Quality ◽

Basal Medium ◽

Adventitious Shoot ◽

Populus Alba ◽

Adventitious Shoot Regeneration

Download Full-text

In Vitro Propagation of Apios americana

HortScience ◽

10.21273/hortsci.25.11.1439 ◽

1990 ◽

Vol 25 (11) ◽

pp. 1439-1440 ◽

Cited By ~ 2

Author(s):

E.R.M. Wickremesinhe ◽

W.J. Blackmon ◽

B.D. Reynolds

Keyword(s):

Gibberellic Acid ◽

In Vitro Propagation ◽

Basal Medium ◽

Shoot Proliferation ◽

Axillary Buds ◽

Ms Medium ◽

Butanoic Acid ◽

Apios Americana

Shoot proliferation from axillary buds of Apios americana Medikus (apios, groundnut) was obtained on a modified Murashige and Skoog (MS) medium supplemented with 2.22 μm BAP, 0.5 μm IBA, and 3.0 μm GA3. Existed shoots rooted on MS basal medium. About 60% of the rooted plants were successfully established in soil. Chemical names used: 1 H-indole-3-butanoic acid (IBA). gibberellic acid (GA3), N6-benzylaminopurine (BAP).

Download Full-text

Adventitious Shoot Regeneration from Leaf Explant of Dwarf Hygro (Hygrophila polysperma(Roxb.) T. Anderson)

The Scientific World JOURNAL ◽

10.1155/2013/680425 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 3

Author(s):

Mehmet Karataş ◽

Muhammad Aasim ◽

Ayşegül Çınar ◽

Muhammet Dogan

Keyword(s):

Medicinal Plant ◽

Shoot Regeneration ◽

Leaf Explants ◽

Adventitious Shoot ◽

Ms Medium ◽

Adventitious Shoot Regeneration ◽

Indian States ◽

And Control ◽

Regenerated Plantlets

Dwarf hygro (Hygrophila polysperma) is an ornamental aquatic plant that changes its leaf colours to pinkish in high light. It is listed as a medicinal plant in medicinal plant lists of Indian states of West Bengal and Karnataka. It is also used as a screening tool for toxicities and a bioindicator to detect and control algae. The study reportedin vitroadventitious shoot regeneration from leaf explants cultured on MS medium containing 0.10–1.60 mg/L Kin/TDZ with or without 0.10 mg/L IBA and 500 mg/L Amoklavin to eradicate endogenic bacterial contamination. Direct adventitious shoot regeneration started within one week from both culture mediums followed by late callus induction which was more prominent on TDZ containing media compared to Kin containing media. Addition of 0.10 mg/L IBA with both Kin and TDZ increased shoot regeneration frequency, mean number of shoots per explant, and mean shoot length. Maximum number of 16.33 and 20.55 shoots per explant was obtained on MS medium containing0.80+0.10 mg/L Kin-IBA and0.10+0.10 mg/L TDZ-IBA, respectively. Regenerated shoots were rooted on MS medium containing 0.20–1.00 mg/L IBA followed by successfull acclimatization in aquariums. Regenerated plantlets were also tested in jars containing distilled water that showed the pH 6–9 for the best plant growth and development.

Download Full-text

Rejuvenation of chicory and lettuce plants following phase change in tissue culture

BMC Biotechnology ◽

10.1186/s12896-019-0557-z ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 1

Author(s):

Anthony J. Conner ◽

Helen Searle ◽

Jeanne M. E. Jacobs

Keyword(s):

Tissue Culture ◽

Shoot Regeneration ◽

Seed Set ◽

Genetic Manipulation ◽

Adventitious Shoot ◽

In Vitro Flowering ◽

Phase Changes ◽

Adventitious Shoot Regeneration ◽

Cell And Tissue Culture

Abstract Background A frequent problem associated with the tissue culture of Compositae species such as chicory (Cichorium intybus L.) and lettuce (Lactuca sativa L.) is the premature bolting to in vitro flowering of regenerated plants. Plants exhibiting such phase changes have poor survival and poor seed set upon transfer from tissue culture to greenhouse conditions. This can result in the loss of valuable plant lines following applications of cell and tissue culture for genetic manipulation. Results This study demonstrates that chicory and lettuce plants exhibiting stable in vitro flowering can be rejuvenated by a further cycle of adventitious shoot regeneration from cauline leaves. The resulting rejuvenated plants exhibit substantially improved performance following transfer to greenhouse conditions, with increased frequency of plant survival, a doubling of the frequency of plants that flowered, and substantially increased seed production. Conclusion As soon as in vitro flowering is observed in unique highly-valued chicory and lettuce lines, a further cycle of adventitious shoot regeneration from cauline leaves should be implemented to induce rejuvenation. This re-establishes a juvenile phase accompanied by in vitro rosette formation, resulting in substantially improved survival, flowering and seed set in a greenhouse, thereby ensuring the recovery of future generations from lines genetically manipulated in cell and tissue culture.

Download Full-text