scholarly journals Stability of PRV Resistance in Transgenic Papaya

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 904A-904
Author(s):  
R. Manshardt ◽  
S. Lius ◽  
D. Gonsalves ◽  
M. Fitch ◽  
J. Slightom ◽  
...  
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Transgene Expression ◽  
Protein Gene ◽  
Papaya Ringspot Virus ◽  
Mendelian Segregation ◽  
Transgenic Papaya ◽  
Aphid Inoculation ◽  
High Level ◽  
Level Resistance

Transgenic papaya lines carrying the coat protein gene (CP) of papaya ringspot virus (PRV) strain HA 5-1 display PRV reactions ranging from complete susceptibility (39-3 & 39-4), to slight delay in onset of symptoms (39-1) and attenuation of symptoms (60-3), to high-level resistance (55-1, 63-1). Normal Mendelian segregation of transgene expression was lost in R1 of 39-3 and 39-4, and inbred R1 60-3 gave an aberrant 1:1 ratio. R0 55-1 plants were resistant in the field (Hawaii) for 2 years following manual and/or aphid inoculation, and the high-level resistance remained stable in the R1 after repeated manual inoculations in the greenhouse and graft inoculation for up to 1 year (Cornell). However, inoculation with PRV HA-Oahu strain produced symptoms in some plants at Cornell (9% after 6 weeks) and in Hawaii (50% after 1 year). Two 55-1 and one 60-3 plant subsequently underwent remission of symptoms and became ELISA-negative (Hawaii). Transmission of PRV isolates from symptomatic 55-1 plants to other CP+ 55-1 bioassay plants was unsuccessful.

Virus Resistant Papaya Plants Derived from Tissues Bombarded with the Coat Protein Gene of Papaya Ringspot Virus

1992 ◽  
Vol 10 (11) ◽  
pp. 1466-1472 ◽  
Author(s):  
Maureen M. M. Fitch ◽  
Richard M. Manshardt ◽  
Dennis Gonsalves ◽  
Jerry L. Slightom ◽  
John C. Sanford
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus

Development of transgene construct using coat protein gene of papaya ringspot virus and its validation

2018 ◽  
Vol 18 (1) ◽  
pp. 92
Author(s):  
Ashwini Kumar ◽  
YB Basavaraj ◽  
Pushpendra Kumar ◽  
Manoj Kumar Yadav ◽  
Prashant Mishra ◽  
...  
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus

Studies on molecular variability of coat protein gene of Papaya ringspot virus-P isolates from India

2019 ◽  
Vol 155 (1) ◽  
pp. 369-376 ◽  
Author(s):  
Rajesh Kumar ◽  
Ashish Srivastava ◽  
Aparana Srivastava ◽  
Shalini Srivastava ◽  
Vivek Prasad ◽  
...  
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Molecular Variability

scholarly journals Sequences of the coat protein gene from brazilian isolates of Papaya ringspot virus

2002 ◽  
Vol 27 (2) ◽  
pp. 174-180 ◽  
Author(s):  
ROBERTO C. A. LIMA ◽  
MANOEL T. SOUZA JR. ◽  
GILVAN PIO-RIBEIRO ◽  
J. ALBERSIO A. LIMA
Keyword(s):  
Coat Protein ◽  
Carica Papaya ◽  
Protein Gene ◽  
Average Degree ◽  
Ringspot Virus ◽  
Plant Genome ◽  
Papaya Ringspot Virus ◽  
Transgenic Papaya ◽  
Cp Gene ◽  
Worldwide Production

Papaya ringspot virus (PRSV) is the causal agent of the main papaya (Carica papaya) disease in the world. Brazil is currently the world's main papaya grower, responsible for about 40% of the worldwide production. Resistance to PRSV on transgenic plants expressing the PRSV coat protein (cp) gene was shown to be dependent on the sequence homology between the cp transgene expressed in the plant genome and the cp gene from the incoming virus, in an isolate-specific fashion. Therefore, knowledge of the degree of homology among the cp genes from distinct PRSV isolates which are present in a given area is important to guide the development of transgenic papaya for the control of PRSV in that area. The objective of the present study was to assess the degree of homology among the PRSV cp genes of several Brazilian isolates of this virus. Papaya and PRSV are present in many different ecosystems within Brazil. Twelve PRSV isolates, collected in eight different states from four different geographic regions, were used in this study. The sequences of the cp gene from these isolates were compared among themselves and to the gene used to generate transgenic papaya for Brazil. An average degree of homology of 97.3% at the nucleotide sequence was found among the Brazilian isolates. When compared to 27 isolates from outside Brazil in a homology tree, the Brazilian isolates were clustered with Australian, Hawaiian, and Central and North American isolates, with an average degree of homology of 90.7% among them.

scholarly journals Papaya Ringspot Virus Coat Protein Gene for Antigen Presentation in Escherichia coli

BMB Reports ◽  
2006 ◽  
Vol 39 (1) ◽  
pp. 16-21 ◽  
Author(s):  
Supawat Chatchen ◽  
Mila Juricek ◽  
Paloma Rueda ◽  
Sunee Kertbundit
Keyword(s):  
Escherichia Coli ◽  
Coat Protein ◽  
Antigen Presentation ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Virus Coat Protein ◽  
Virus Coat

scholarly journals 634 Mechanism and Stability of Virus Resistance in Transgenic Papaya

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 557A-557
Author(s):  
Richard Manshardt ◽  
Dennis Gonsalves
Keyword(s):  
New York ◽  
Protein Gene ◽  
Resistance Mechanism ◽  
Local Strains ◽  
Transgenic Papaya ◽  
Challenge Virus ◽  
Loss Of Resistance ◽  
High Level ◽  
Virus Strains ◽  
Level Resistance

Transgenic papaya line 55-1 with resistance to papaya ringspot virus (PRSV) originated in 1989 by particle bombardment of cultivar Sunset with the coat protein gene (cp) of mild mutant Hawaii PRSV strain HA 5-1. Hemizygous (+/cp) R0 clones of 55-1 displayed resistance to the virulent Hawaii HA strain in greenhouse tests in New York in 1991 and to local strains in a field trial in Hawaii from 1992 to 1994. In the R1 generation produced by crossing the pistillate R0 55-1 with `Sunset', up to 50% of the hemizygous transgenic segregants were susceptible to a local Oahu PRSV strain when inoculated as seedlings but not as mature plants. Similar inoculation experiments in New York showed that hemizygous R1 transgenics were susceptible in differing degrees to PRSV strains from regions other than Hawaii. Homozygous (cp/cp) R2, R3, and R4 populations planted in various locations in Hawaii since 1994 have consistently demonstrated high-level resistance to local strains at all stages of development. When inoculated in New York with eight non-Hawaii PRSV strains, homozygous R3 seedlings were resistant to all but a Thai strain. Transgenic resistance is the result of a complex interaction involving the stage of plant development, transgene dosage, the degree of homology between transgene and challenge virus, and environmental variables. Papaya plants transformed with nontranslatable versions of various cp genes are also highly resistant to PRSV, indicating that the resistance mechanism operates at the RNA level. No loss of resistance due to the appearance of resistance-breaking virus strains or to transgene inactivation has been noted thus far.

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