scholarly journals Chromosome Numbers of Rubus Cultivars at the National Clonal Germplasm Repository

HortScience ◽  
1995 ◽  
Vol 30 (7) ◽  
pp. 1453-1456 ◽  
Author(s):  
Maxine M. Thompson

Chromosome numbers were counted for 90 Rubus cultivars and selections maintained at the U.S. Dept. of Agriculture, Agricultural Research Service, National Clonal Germplasm Repository, Corvallis, Ore. To my knowledge, 37 of the counts are new, including five that are corrections of previously published counts, 30 that are confirmations of numbers that were previously published but assumed from their parentage rather than actually counted, and 23 that are confirmations of previous counts. The basic number was 7, and 2n numbers ranged from 2x to 14x, including odd-ploids and aneuploids.

HortScience ◽  
1995 ◽  
Vol 30 (7) ◽  
pp. 1447-1452 ◽  
Author(s):  
Maxine M. Thompson

The U.S. Dept. of Agriculture, Agricultural Research Service, National Clonal Germplasm Repository (NCGR), Corvallis, Ore., maintains Rubus germplasm representing worldwide diversity of the genus. Chromosome numbers were counted for 201 plants representing 124 taxa (species and varieties). There are new reports for 42 taxa, confirmation for 72 previously reported, and 10 counts for plants unidentified to species. The basic chromosome number was seven, and ploidy levels ranged from 2x to 12x.


HortScience ◽  
2012 ◽  
Vol 47 (12) ◽  
pp. 1837-1842 ◽  
Author(s):  
Mark K. Ehlenfeldt ◽  
Matthew Kramer

Rabbiteye blueberry hybrids that the U.S. Department of Agriculture, Agricultural Research Service (USDA-ARS) program has bred for northern adaptation are combinations of 6x V. ashei Reade, 6x V. constablaei Gray, 4x V. corymbosum L., and 2x V. darrowii Camp germplasm at the hexaploid level and are generally composed of 50% or greater V. ashei (rabbiteye) germplasm. Four northern-adapted rabbiteye (NRE) selections (US 1043, US 1045, US 1056, US 1057), four rabbiteye standards (‘Brightwell’, ‘Climax’, ‘Tifblue’, ‘Woodard’), two rabbiteye × V. constablaei derivatives (‘Little Giant’, ‘Snowflake’), and two highbush standards (‘Duke’, ‘Bluecrop’) were pollinated under greenhouse conditions with either self-pollen or a multicultivar, bulk-pollen mixture (appropriate to ploidy level and species) to determine the relative requirements for cross-pollination among NRE selections. Fruit set, berry weight, and seed set were subsequently evaluated. The results suggest that NRE selections, in general, exhibit cross-pollination needs intermediate to the parent types such that: rabbiteye > northern rabbiteye > highbush (i.e., rabbiteye has the lowest self-fertility and the greatest need for cross-pollination). Considerable variation existed among the NRE selections tested, which suggests that it might be possible to select clones with good levels of self-fertility, potentially equivalent to that of highbush blueberry.


HortScience ◽  
2016 ◽  
Vol 51 (7) ◽  
pp. 816-821 ◽  
Author(s):  
Chunxian Chen ◽  
William R. Okie ◽  
Thomas G. Beckman

Peach fruit set is affected by cumulative chill and spring frost. A spring frost occurred on 29 Mar. 2015 at the U.S. Department of Agriculture, Agricultural Research Service (USDA-ARS) Byron station after 3 weeks of bloom, reducing fruit set and resulting in many buttons (abnormally small fruit with dead embryos). Fruit set was rated in 2014, 2015, and 2016 and button set rated in 2015 using the same scale (0 = no fruit/button to 9 = 1–2 fruit/button at every node). The overall fruit set rating was substantially different in the 3 years, averaging 5.61 in 2014, 2.61 in 2015, and 6.04 in 2016. Buttons and skin-damaged fruit in 2015 varied among peach genotypes. Comparison of fruit and button set ratings showed that there was no difference between cultivars and selections, but some significant differences in fruit set for four ripening months, among the 3 years, and among the nine chilling classes, respectively. Among the cultivars, the most common button set rating was 0–3. For example, ‘Sunprince’, ‘Loring’, and ‘Carored’ trees had a high button set rating, whereas ‘Flameprince’, ‘Julyprince’, and ‘Contender’ trees were low. As for peach selections, BY04P1690n was among those with the highest button set rating. In the population derived from a cross of button-prone BY04P1690n and button-free BY99P3866w, fruit and button counts from 10 long fruiting shoots ranged from 4 to 53 fruit (21.63 on average) and 2 to 27 buttons (10.39 on average). The peach button rate ranged from 5.36% to 87.10% (30.70% on average). The range, distribution, and percentage of the button counts suggested that, if buttoning was genetically controlled, it appeared quantitative. Further assessment is needed.


2002 ◽  
Vol 65 (3) ◽  
pp. 567-570 ◽  
Author(s):  
JOHN B. LUCHANSKY ◽  
ANNA C. S. PORTO ◽  
F. MORGAN WALLACE ◽  
JEFFREY E. CALL

This study compared three methods for the recovery of Listeria monocytogenes from commercially prepared and vacuum-packaged frankfurters that were inoculated with a five-strain mixture of this pathogen at averages of 22 and 20,133 CFU per package over three trials. The presence and levels of the pathogen were determined by (i) the U.S. Department of Agriculture (USDA) Food Safety and Inspection Service (FSIS) product composite enrichment method, involving the selective enrichment of a 25-g composite of product and the subsequent plating of this product onto selective agar plates; (ii) the USDA Agricultural Research Service (ARS) product composite rinse method, involving the rinsing of a 25-g composite of product with 0.1% peptone water and the subsequent plating of a portion of the rinse fluid directly onto selective agar plates; and (iii) the USDA-ARS package rinse method, involving the use of 25 ml of 0.1% peptone water to rinse the entire contents of a package and the subsequent plating of a portion of the rinse fluid directly onto selective agar plates. For packages inoculated with 20,133 CFU, L. monocytogenes was recovered at a frequency (percentage of packages positive) of 100% by each of the three methods. The pathogen was recovered at efficiencies (percentages of recovery of L. monocytogenes) of 43 and 94% with the USDA-ARS product rinse method and the USDA-ARS package rinse method, respectively. For packages inoculated with 22 CFU, L. monocytogenes was recovered at frequencies of 17, 10, and 100% by the USDA-FSIS product composite enrichment method, the USDA-ARS product composite rinse method, and the USDA-ARS package rinse method, respectively. The pathogen was recovered at efficiencies of 20 and 95% with the USDA-ARS product composite rinse method and the USDA-ARS package rinse method, respectively. In a related study, the USDA-ARS package rinse method was the only method that detected the pathogen in 60 packages from each of five brands of frankfurters purchased from local grocery stores. These data establish that the USDA-ARS package rinse method is markedly more sensitive, as well as demonstrably more rapid and facile, than either the approved USDA-FSIS product composite enrichment method or the USDA-ARS product composite rinse method in determining the presence or absence of L. monocytogenes and establishing the levels of the pathogen that may be on the surface of ready-to-eat foods such as frankfurters.


HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 395A-395
Author(s):  
Kim E. Hummer

In 1999, the National Clonal Germplasm Repository at Corvallis, Ore., was assigned to preserve the hardy Actinidia Lindl. resources for the U.S. Dept. of Agriculture, Agricultural Research Service, National Plant Germplasm System. The fuzzy kiwifruit [A. deliciosa (A. Chev) C. F. Liang et A. R. Furguson] and other less cold-hardy Actinidia species, remain at the Davis Repository. The hardy Actinidia, commonly called Chinese gooseberries or hardy kiwifruit, encompass two taxonomic sections, Leiocarpae and Maculatae, and include about 13 described species. These perennial vines are natives of Asia and have been developed and cultivated in Lushan, Wuhan, and Guilin, China; Motueka, New Zealand; Kagawa Prefecture, Japan; Vladivostok, Russia; and California, Maryland, Michigan, Minnesota, New Hampshire, and New York in the United States. Thus far, the Corvallis Repository has established representatives of six species, A. arguta (Siebold & Zucc.) Planch. Ex Miq., A. callosa Lindl., A. kolomikta (Maxim.&Rupr.) Maxim, A. melanandra Franch., A. polygama (Siebold & Zucc.) Maxim., A. purpurea Rehder and 60 cultivars. These clones will be preserved as potted plants under screen. They will also be fruited and evaluated as trellised plants in the field. The repository plans to expand the species diversity of the collections. Plant requests for dormant scionwood or spring softwood cuttings are available by contacting the Corvallis Repository Curator.


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