Identification of RAPD Markers Linked to Major Gene for Indeterminate Growth Habit using Bulked Segregant Analysis in Common Bean Cross

Plant growth habit is an important trait. Our objective was to identify RAPD markers linked to major gene for indeterminate growth habit using bulked segregant analysis in an F2 population from a bean cross Chichara (indeterminate growth habit × PC-50 (determinate growth habit). A total of 132 RAPD primers (600 RAPD primer screened) showed polymorphisms between bulked DNA derived from indeterminate and determinate plants. All markers showed coupling linkage with indeterminate growth habit. RAPD markers of A-8, A-17, C-7, C-15, D-4, D-5, F-6, F-16, G-9, H-3, H-20, and I-7 were 2.2 cM distant from the gene for indeterminate growth habit. Markers of B-7, B-16, B-17, C-8, E-1, F-1, F-20 and H-l9 primers were 4.6 cM distant from the gene for indeterminate growth habit.

Download Full-text

Molecular tagging of photoperiod responsive flowering in Indian bean [Lablab purpureus (L.) Sweet] Vinita Ramtekey*, Arpit Bhuriya, Dipendra Ayer, Vipulkumar Parekh, Kaushal Modha, Bhushan Kale, Gopal Vadodariya and Ritesh Patel (

Indian Journal of Genetics and Plant Breeding (The) ◽

10.31742/ijgpb.79s.1.17 ◽

2019 ◽

Vol 79 (01S) ◽

Author(s):

Vinita Ramtekey ◽

Arpit Bhuriya ◽

Dipendra Ayer ◽

Vipulkumar Parekh ◽

Kaushal Modha ◽

...

Keyword(s):

Common Bean ◽

Growth Habit ◽

Lablab Purpureus ◽

Indeterminate Growth ◽

F1 Hybrid ◽

F2 Population ◽

Indeterminate Growth Habit ◽

Determinate Growth ◽

Determinate Growth Habit ◽

F2 Generation

Photoperiod responsive flowering and growth habit might have played a key role in domestication of lablab bean (Lablab purpureus) and currently shifting its cultivation from intercropping to monoculture. Most of the landraces of lablab bean exhibit photoperiod sensitivity in flowering and indeterminate growth habit. A cross was made between GNIB21 and GP189 which are phenotypic extremes for photoperiod responsive flowering. The F1 hybrid exhibited dominant traits like indeterminate growth habit and photosensitive flowering endowed from male parent. Segregation pattern of 3:1 in F2 generation indicated monogenic recessive nature of photoperiod insensitive flowering. Bulk segregant analysis in F2 population revealed association of PvTFLy1, a locus governing determinate growth habit in lablab bean, with photoperiod responsive flowering where an amplicon of 300 bp was observed in photo sensitive GP189 while it was absent in photo insensitive variety GNIB21. Significant ÷2 test indicated coupling phase of linkage between PvTFLY1 and photoperiod responsive flowering. Linkage analysis placed PvTFLY1 at the distance of 19.23 cM from the locus governing photoperiod responsive flowering. The linkage between growth habit and photoperiod responsive flowering in common bean, soybean and Indian bean suggest that these traits may be governed by mutation or deletion of E3 (or GmPhyA3) and Dt1 homologs in Indian bean. Information available on characterized genes for photoperiod responsive flowering and determinate growth habit from common bean, soybean and other related legumes may be utilized for isolation, characterization, mapping and molecular dissection of genes involved in regulation of photoperiod responsive flowering in Indian bean.

Download Full-text

DEFOLIATION LEVELS AT VEGETATIVE AND REPRODUCTIVE STAGES OF SOYBEAN CULTIVARS WITH DIFFERENT RELATIVE MATURITY GROUPS

Revista Caatinga ◽

10.1590/1983-21252020v33n213rc ◽

2020 ◽

Vol 33 (2) ◽

pp. 402-411

Author(s):

MURILO MIGUEL DURLI ◽

LUIS SANGOI ◽

CLOVIS ARRUDA SOUZA ◽

LUCIELI SANTINI LEOLATO ◽

THAIS LEMOS TUREK ◽

...

Keyword(s):

Grain Yield ◽

Growth Habit ◽

Leaf Expansion ◽

Maturity Group ◽

Indeterminate Growth ◽

Soybean Cultivars ◽

Indeterminate Growth Habit ◽

Determinate Growth ◽

Determinate Growth Habit ◽

Expansion Capacity

ABSTRACT Soybean tolerance to defoliation may be affected by relative maturity group (RMG) of the cultivar. For this reason, this study was carried out to evaluate the effects of artificial defoliation at vegetative and reproductive stages on the agronomic performance of soybean cultivars with different RMG. Two experiments were carried out in greenhouse conditions in Lages, Santa Catarina State, Brazil. Defoliation was carried out at V6 in the first experiment and at R3 in the second experiment. Three cultivars with contrasting RMG were used: BMX Veloz (RMG of 5.0, indeterminate growth habit), NA 5909 (RMG of 5.9, indeterminate growth habit), and TMG 7262 (RMG of 6.2, semi-determinate growth habit). Each cultivar was submitted to five defoliation levels: 0.0, 16.6, 33.3, 50.0, and 66.6%. Leaf expansion after defoliation, grain yield and its components were determined. The BMX Veloz showed lower leaf expansion capacity and lower grain yield when compared to the other cultivars when defoliated at V6. Grain yield of the three cultivars only showed significant decreases with defoliation levels higher than 16.6% at R3. The cultivar BMX Veloz is less tolerant to defoliation during the vegetative stage when compared to the cultivars NA 5909 and TMG 7262. Cultivar maturity group does not interfere with soybean tolerance to defoliation at the reproductive stage in greenhouse conditions.

Download Full-text

Tomato production in hydroponic system using different agrofilms as greenhouse cover

Horticultura Brasileira ◽

10.1590/s0102-053620200109 ◽

2020 ◽

Vol 38 (1) ◽

pp. 58-64

Author(s):

Douglas José Marques ◽

Edmur Matheus Filho ◽

Hudson C Bianchini ◽

Valdir Veroneze Junior ◽

Breno Régis Santos ◽

...

Keyword(s):

Gas Exchange ◽

Crop Production ◽

Growth Habit ◽

Soluble Solids ◽

Tomato Crop ◽

Indeterminate Growth ◽

Tomato Production ◽

Hydroponic System ◽

Indeterminate Growth Habit ◽

Lycopene Content

ABSTRACT Protected cultivation has increased over the years. More studies on the benefits of using photoselective agrofilms are necessary. The choice of material to cover the greenhouse is a decisive factor for crop development and production. The aim of this study was to evaluate the effect of diffuser and blue-colored agrofilms on the production of different tomato cultivars of indeterminate growth habit under NFT hydroponic system. A completely randomized design in a 2x3 factorial scheme (two environments and three cultivars) was used: two agrofilm types (blue and diffuser) and 3 cultivars (Monterrey, Arendell and Totalle). Each plot consisted of 30 plants per treatment, with four replicates. Commercial tomato seedlings of indeterminate growth habit grafted on rootstock cultivar (Shincheonggang) were used. During the experiment, the electrical conductivity, intensity of radiation inside the greenhouse, gas exchange, soluble solids, lycopene content, pH and fruit production were evaluated. We concluded that the amount of radiation transmitted through the diffuser favors an increase of about 18% in gas exchange, 12% in lycopene content and 9.4% in tomato crop production.

Download Full-text

Developing RAPD Markers for Grape Breeding

HortScience ◽

10.21273/hortsci.31.4.609f ◽

1996 ◽

Vol 31 (4) ◽

pp. 609f-609 ◽

Cited By ~ 1

Author(s):

J. Lu ◽

O. Lamikanra ◽

Y. Wang ◽

Z. Liu ◽

D. Ramming

Keyword(s):

Rapd Markers ◽

Scar Marker ◽

Bulked Segregant Analysis ◽

Rapd Marker ◽

Early Screening ◽

Horticultural Crop ◽

Pooled Dna ◽

Rapd Primer ◽

Breeding Selection ◽

Grape Breeding

The grape is an important horticultural crop that is grown worldwide. Breeding a new grape cultivar by conventional means normally will take several generations of backcross, at least 15 years. The efficiency and speed of selection can be accelerated if genetic markers are available for early screening. This project is designed to generate RAPD markers linked to viticulturally important traits, including seedlessness and pistillate genes. A F1 population with 64 progenies of V. vinifera was used for the RAPD analysis. Bulked Segregant Analysis (BSA) method was used for RAPD primer screening. Three-hundred primers were screened between the two pairs of pooled DNA samples, seeded and seedlessness, pistillate and perfect flowers. At least 10 primers produced one polymorphism each between the pools. Further analysis revealed that one of these RAPDs cosegregated tightly with the seedlessness trait, while the others either had loose linkage or no linkage to the traits. To make the RAPD marker useful for breeding selection, an attempt was made to convert it into SCAR marker. The results demonstrated that the RAPD marker may be useful for grape breeding and interpreting inheritance of a particular trait in grapes.

Download Full-text

Studies on the Significance of the Indeterminate : Growth Habit in Breeding Soybeans : 1. Properties of American soybeans attributable to their indeterminate growth habits

Ikushugaku zasshi ◽

10.1270/jsbbs1951.11.24 ◽

1961 ◽

Vol 11 (1) ◽

pp. 24-28

Author(s):

Tadao Nagata

Keyword(s):

Growth Habit ◽

Indeterminate Growth ◽

Indeterminate Growth Habit ◽

Growth Habits

Download Full-text

STUDIES ON THE SIGNFICANCE OF THE INDETERMINATE GROWTH HABIT IN BREEDING SOYBEANS. : VII Some Dynamic Considerations on the Difference in Lodging Resistance between the Determinate and the Indeterminate Varieties.

Ikushugaku zasshi ◽

10.1270/jsbbs1951.18.291 ◽

1968 ◽

Vol 18 (5) ◽

pp. 291-298

Author(s):

Tadao Nagata

Keyword(s):

Growth Habit ◽

Lodging Resistance ◽

Indeterminate Growth ◽

Indeterminate Growth Habit ◽

The Difference

Download Full-text

INDUSTRIAL TOMATO FOR FRESH CONSUMPTION: A TECHNICAL AND ECONOMICALLY VIABLE ALTERNATIVE

JOURNAL OF NEOTROPICAL AGRICULTURE ◽

10.32404/rean.v7i3.3823 ◽

2020 ◽

Vol 7 (3) ◽

pp. 20-28

Author(s):

Daianna Pereira Costa ◽

Juliana Nascimento Da Silva ◽

Donielsin Da Silva Assunção ◽

Fritz Mohn Penteado ◽

Abadia Dos Reis Nascimento

Keyword(s):

Experimental Design ◽

Production Cost ◽

Growth Habit ◽

Viable Alternative ◽

Economic Viability ◽

Indeterminate Growth ◽

Tomato Production ◽

Indeterminate Growth Habit ◽

Lower Production ◽

Tomato Genotypes

The cultivation of hybrid tomatoes, intended for fresh consumption, with a determined growth habit, and without support, is an alternative that has a lower production cost. The research aimed to evaluate the productivity and economic viability of different tomato genotypes, with a determinate and indeterminate growth habit. The experimental design was in completely randomized blocks. Ten hybrids and a variety were grown in 2016, and nine hybrids, and a variety in 2018, with a spacing of 0.5 meters between plants. Harvests were carried out in four periods in 2016, at 72, 82, 97, and 105 days after transplanting (DAT); and in 2018 at 81, 87, 97, and 111 DAT. After harvesting, the gross value of production and profit were quantified. The hybrids that obtained the highest production per plant in 2016 were Gabrielle (6,010 kg), with support; Thaise (5.529 kg); and N901 (5.003 kg). In 2018, Dominador and Thaise hybrids had the highest production, with 4.109 and 3.961 kg per plant, respectively. The gross value of tomato production in 2016 was higher than in 2018 for all hybrids and variety, except for Dominador hybrid. Hybrids with a determined growth habit and conducted without support are a profitable alternative to produce tomatoes intended for fresh consumption, especially the hybrid Gabrielle, which had the highest production. The economic viability of hybrids grown without support is superior to all hybrids with support.

Download Full-text