scholarly journals Small Inflorescence Bulbils Are Best for Micropropagation and Virus Elimination in Garlic

HortScience ◽  
2000 ◽  
Vol 35 (4) ◽  
pp. 735-737 ◽  
Author(s):  
M. Ebi ◽  
N. Kasai ◽  
K. Masuda
Keyword(s):  
Mosaic Virus ◽  
Allium Sativum ◽  
Naphthaleneacetic Acid ◽  
Virus Elimination ◽  
Immature Inflorescence ◽  
Immunoblot Assay ◽  
Murashige And Skoog's Medium ◽  
Murashige And Skoog’S Medium ◽  
Remarkable Reduction

Garlic (Allium sativum L.) plants were cultured from immature inflorescence bulbils. The primordia of bulbils appeared initially as protuberances on reproductive apices, swelled, and then formed bulbils with protective leaves. Excised bulbils sprouted on Murashige and Skoog's medium with 5.4 μm naphthaleneacetic acid (NAA) and grew into plantlets. The frequency of sprouting in culture increased with the development of bulbils before excision, and immature bulbils 0.4 to 2.4 mm in diameter sprouted at a frequency of >85%. A dot immunoblot assay revealed a remarkable reduction in levels of garlic mite-borne mosaic virus in plantlets grown from immature bulbils, suggesting that such bulbils might be suitable for the propagation of virus-free garlic plants.

Conditions d'apparition d'une embryogénèse somatique sur des cals issus de la culture de tissus foliaires du chêne vert (Quercus ilex)

1989 ◽  
Vol 67 (4) ◽  
pp. 1066-1070 ◽  
Author(s):  
C. Féraud-Keller ◽  
H. Espagnac
Keyword(s):  
Acetic Acid ◽  
Somatic Embryos ◽  
Quercus Ilex ◽  
Foliar Tissue ◽  
Murashige And Skoog's Medium ◽  
Old Trees ◽  
Murashige And Skoog’S Medium

The proliferation of parenchymatous foliar tissue of Quercus ilex was obtained from current year leaves taken from old trees and cultivated in vitro on modified Murashige and Skoog's medium supplemented with benzylaminopurine (4 mg ∙ L−1) and naphthyl acetic acid (0.5 mg ∙ L−1). Only the fragments cultured in October reacted. The neoformations only appeared on calluses that had not been subcultured for 7 months. Primary nodules arising on these calluses were removed and subcultured on the same medium either in the dark or in the light. In the dark only, they produced secondary nodules, which were the source of somatic embryos both in light and dark. Presently, they seem to regulate their structure in the dark but they do not develop in a way that leads to germination.

scholarly journals Callus multiplication of Anthurium andraeanum Lind. in liquid media.

1975 ◽  
Vol 23 (4) ◽  
pp. 299-302
Author(s):  
R.L.M. Pierik
Keyword(s):  
Callus Tissue ◽  
Rotation Speed ◽  
Multiplication Rate ◽  
Fresh Weight ◽  
Liquid Media ◽  
Continuous Darkness ◽  
Anthurium Andraeanum ◽  
Murashige And Skoog's Medium ◽  
Murashige And Skoog’S Medium ◽  
Genotype A

The growth of callus tissue from adult A. andraeanum plants was best in a modification of Murashige and Skoog's medium. On this medium genotype A 42-3 reached a fresh weight multiplication rate of 30.7 when grown for 5 weeks at a rotation speed of 120 rev/min, at 25 deg C and in continuous darkness. The fresh weight multiplication rate of 6 other genotypes of the same species varied considerably when grown on the best medium for A 42-3. (Abstract retrieved from CAB Abstracts by CABI’s permission)

Plantlet formation from Lycopersicon esculentum leaf callus

1974 ◽  
Vol 52 (6) ◽  
pp. 1429-1432 ◽  
Author(s):  
Vasantha Padmanabhan ◽  
E. F. Paddock ◽  
W. R. Sharp
Keyword(s):  
Acetic Acid ◽  
Lycopersicon Esculentum ◽  
Shoot Formation ◽  
Growth Hormones ◽  
Root Initiation ◽  
Plantlet Formation ◽  
Callus Proliferation ◽  
Murashige And Skoog's Medium ◽  
Murashige And Skoog’S Medium ◽  
Indole 3 Acetic Acid

Explants were obtained from leaves of three strains of tomato and grown on a modified Murashige and Skoog's medium with various combinations of indole-3-acetic acid (IAA) and kinetin. Callus proliferation began by 8–10 days. Root initiation was very common, particularly at 2 mg/liter IAA and 2 mg/liter kinetin. Shoot formation occurred within 30 days but only at a specific combination of concentrations of the two growth hormones (4 mg/liter IAA + 4 mg/liter kinetin). Most shoots became plantlets by 10 days after transfer to basal Murashige and Skoog's medium. Shoot-forming potential was neither correlated with callus-forming potential nor with vigor of strain.

scholarly journals Characterization of a New Virus from Garlic(Allium sativum L.), Garlic Mite-borne Mosaic Virus.

1996 ◽  
Vol 62 (5) ◽  
pp. 483-489 ◽  
Author(s):  
Kazuo YAMASHITA ◽  
Junichi SAKAI ◽  
Kaoru HANADA
Keyword(s):  
Mosaic Virus ◽  
Allium Sativum

scholarly journals Improving Multiple Shoot Proliferation in Bamboo Mosaic Virus-free Bambusa oldhamii Munro Propagation by Liquid Culture

HortScience ◽  
2007 ◽  
Vol 42 (5) ◽  
pp. 1243-1246 ◽  
Author(s):  
Choun-Sea Lin ◽  
Krishnan Kalpana ◽  
Wei-Chin Chang ◽  
Na-Sheng Lin
Keyword(s):  
Tissue Culture ◽  
Mosaic Virus ◽  
Liquid Culture ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Naphthaleneacetic Acid ◽  
Semisolid Medium ◽  
Bambusa Oldhamii

An in vitro method for obtaining bamboo mosaic virus (BaMV)-free plantlets of Bambusa oldhamii Munro was developed. BaMV-free meristems were incubated on MS basal medium supplemented with 0.45 μm thidiazuron (TDZ) to induce the development of multiple shoots. Multiple shoot proliferation was higher in stationary liquid culture than on semisolid medium. Cytokinin was the key component for inducing proliferation, and TDZ was the stable and effective cytokinin for proliferation in long-term subcultures. Multiple shoots rooted after 1 month in MS basal medium containing 10.74 to 26.85 μm α-naphthaleneacetic acid with a rooting efficiency of 83%. Healthy, well-developed plantlets were transferred to soil in pots and raised in a greenhouse. Those plants derived from tissue culture were more vigorous than the ones derived from the traditional in vivo vegetative propagation method, air layering. The tissue culture-derived plants could produce the culms after 15 months. Fifteen of 38 plants flowered 2 years after being transplanted to the field.

scholarly journals Micropropagation, genetic fidelity assessment and phytochemical studies of Clerodendrum thomsoniae Balf. f. with special reference to its anti-stress properties

2019 ◽  
pp. 09-15 ◽  
Author(s):  
Pallab Kar ◽  
Arnab Kumar Chakraborty ◽  
Malay Bhattacharya ◽  
Tanmayee Mishra ◽  
Arnab Sen
Keyword(s):  
Special Reference ◽  
Genetic Fidelity ◽  
Fidelity Assessment ◽  
Callus Regeneration ◽  
Murashige And Skoog's Medium ◽  
Murashige And Skoog’S Medium ◽  
Phytochemical Studies ◽  
New Crop ◽  
Nodal Culture

Clerodendrum thomsoniae commonly known as bleeding heart vine or bag flower which is a good candidate for a new crop for the floriculture industry. In this study, in-vitro callus regeneration of C. thomsoniae through nodal culture has been attempted. Murashige and Skoog’s medium supplemented with 2 mg/l BAP and 0.5 mg/l

scholarly journals Influence of explant size, heat treatment, and variety on garlic (Allium sativum L) meristematic tissue growth

2021 ◽  
Vol 306 ◽  
pp. 01036
Author(s):  
Asih K. Karjadi ◽  
Nazly Aswani
Keyword(s):  
Heat Treatment ◽  
Growth And Development ◽  
Allium Sativum ◽  
Tissue Growth ◽  
Onion Yellow Dwarf Virus ◽  
Virus Elimination ◽  
Meristematic Tissue ◽  
Yellow Dwarf Virus ◽  
Das Elisa ◽  
Virus Testing

Garlic (Allium sativum L) is one of genus Allium sp which propagated vegetatively. In vegetative propagation, virus is one of the major diseases. However, it has been approved that OYDV (Onion Yellow Dwarf Virus) can be eliminated through unconventional propagation techniques. The aim of this research was to evaluate the effect of explant, variety and heat treatment on the growth and development of explants dealing with virus elimination. The research was conducted on the IVEGRI tissue culture laboratory from May to September 2018. The research was designed using combination between explant types e.g. meristem (E1) and shoot tip (E2), heat treatment (370C) for 1 month, and varieties e.g. Lumbu Hijau (V1); Tawang Mangu (V2); Lumbu Putih (V3). The composition of media consisted of MS + MS vitamine + Sucrose 30 g L-1+ IAA 2 mg L-1 + Kinetin 2 mgL-1 + GA30.01 mgL-1 + Gel gro 2 gL-1, pH 5.7 were used as media growth. The results concluded that explant size, heat treatment and varieties affected percentage of proliferation. The bigger the explant size used, the higher growth percentage resulted. From the average number of shoots, leaves and roots, it also suggested that explant type and varieties affected the explant growth and development. Moreover, it was shown that the culture contamination on 10 WAP ranged from 20% to 55% which generally caused by bacteria. The results of virus testing OYDV on plantlets using DAS ELISA Serology technique showed that the infected cultures ranged from 22.22% to 60 %.

scholarly journals Shoot Regeneration Rates of Perennial Phlox are Dependant on Cultivar and Explant Type

HortScience ◽  
2004 ◽  
Vol 39 (6) ◽  
pp. 1373-1377 ◽  
Author(s):  
Margarita Fraga ◽  
Mertxe Alonso ◽  
Marisé Borja
Keyword(s):  
Adventitious Shoots ◽  
Leaf Explants ◽  
Naphthaleneacetic Acid ◽  
Meristem Culture ◽  
Ms Medium ◽  
Virus Elimination ◽  
Open Flower ◽  
Regeneration Rate ◽  
Shoot Initiation

Meristem culture and/or thermotherapy were used for virus elimination from ornamental Phlox paniculata L. (`Blue Boy', `Orange perfection' and `Starfire') mother plants. Shoot tip, leaf, node and flower ovary explants collected from greenhouse-maintained virus free plants were cultured in vitro for shoot initiation. Adventitious shoot initiation was observed on Murashige and Skoog (MS) medium containing the cytokinin BA with or without the auxin NAA. The addition of 0.4 mg·L-1 thiamine, 0.4 mg·L-1 folic acid, and 40 mg·L-1 adenine sulfate to the MS medium did not improve the regeneration rate. Multiplication and rooting were genotype dependent. Blue Boy and Orange Perfection cultivars regenerated the maximum number of shoots from leaf explants. `Blue Boy' leaf explants from in vitro plants had a lower regeneration rate than explants from greenhouse plants. Cultivar `Starfire' had the highest shoot formation with open flower ovary explants and failed to regenerate from leaf explants. In vitro rooting of adventitious shoots in the presence of auxins (IAA, NAA, or IBA) with or without BA was less effective than ex vitro rooting. Chemical names used: 6-benzyladenine (BA); indole-acetic acid (IAA); indole-3-butyric acid (IBA); α-naphthaleneacetic acid (NAA).

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