scholarly journals KOMBINASI TERMOTERAPI DAN KHEMOTERAPI DENGAN KULTUR APEKS DAN MERISTEM UNTUK ELIMINASI VIRUS MOSAIK PADA TEBU / The Combined Treatment of Thermotherapy and Chemotherapy with Apex and Meristem Culture for Mosaic Virus Elimination in Sugarcane

2016 ◽  
Vol 22 (1) ◽  
pp. 19
Author(s):  
Ika Roostika ◽  
SEDYO HARTONO ◽  
DARDA EFENDI
Keyword(s):  
Mosaic Virus ◽  
Combined Treatment ◽  
Meristem Culture ◽  
Virus Elimination

scholarly journals Virus elimination in potato through meristem culture followed by thermotherapy

2014 ◽  
Vol 11 (1) ◽  
pp. 71-80 ◽  
Author(s):  
MA Ali ◽  
KM Nasiruddin ◽  
MS Haque ◽  
SM Faisal
Keyword(s):  
Gibberellic Acid ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Combined Treatment ◽  
Meristem Culture ◽  
Virus Elimination ◽  
Potato Varieties ◽  
Virus Indexing ◽  
Plantlet Production ◽  
Benzyl Amino Purine

Virus elimination in potato through meristem culture followed by thermotherapy and virus indexing was studied. Three levels of thermotherapy, viz. 27±1°C (control), 30±1°C and 35±1°C, sixteen combinations of BAP (Benzyl Amino Purine) plus GA3 (gibberellic Acid) concentrations viz. 0.0+0.0 (control), 0.0+0.2, 0.0+0.4, 0.0+0.6, 1.5+0.0, 1.5+0.2, 1.5+0.4, 1.5+0.6, 3.0+0.0, 3.0+0.2, 3.0+0.4, 3.0+0.6 , 4.5+0.0, 4.5+ 0.2, 4.5+0.4 and 4.5+0.6 were used in this study in three potato varieties viz. Diamant, Heera and Lalpakri. Among the thermo therapies, 27±1°C showed the highest (24.55) survival response followed by 30±1°C, 35±1°C, respectively. The poorest (20.47) survival response of meristem derived plantlets was noticed in 35±1°C which gave the highest percentage (43.79) of virus free plantlets followed by 30±1°C. The best (25.85%) survival response was found in Lalpakri and the lowest (19.08%) survivality was recorded in Diamant. The highest (33.27) percentage of PVY (Potato Virus Y) free plantlets was observed in Heera. The combined treatment 3.0 mg L-1 BAP and 0.2 mg L-1 GA3 showed the highest (63.39) percentage of virus free plantlet production followed by 4.5 mg L-1 BAP and 0.2 mg L-1 GA3DOI: http://dx.doi.org/10.3329/sja.v11i1.18376 SAARC J. Agri., 11(1): 71-80 (2013)

Combined effect of chemotherapy and meristem culture on sugarcane mosaic virus elimination in sugarcane

Sugar Tech ◽  
2002 ◽  
Vol 4 (1-2) ◽  
pp. 19-25 ◽  
Author(s):  
M. Balamuralikrishnan ◽  
Sabitha Doraisamy ◽  
T. Ganapathy ◽  
R. Viswanathan
Keyword(s):  
Mosaic Virus ◽  
Combined Effect ◽  
Sugarcane Mosaic Virus ◽  
Meristem Culture ◽  
Virus Elimination

scholarly journals IN VITRO ELIMINATION OF DAHLIA MOSAIC VIRUS BY USING MERISTEM CULTURE, ELECTROTHERAPY AND CHEMOTHERAPY

2020 ◽  
Vol 51 (2) ◽  
pp. 665-674
Author(s):  
Nerway & et al.
Keyword(s):  
Salicylic Acid ◽  
Mosaic Virus ◽  
Meristem Culture ◽  
Ms Medium ◽  
Virus Elimination ◽  
In Vitro Techniques ◽  
Dahlia Mosaic Virus ◽  
Time Courses ◽  
Das Elisa

This study was conducted to investigate the effect of different in vitro techniques on elimination of dahlia mosaic virus. Meristems of Dahlia mosaic virus (DMV) infected dahlias, with 0.2-0.3 mm in length, were cultured on Murashige and Skoog (MS) medium. Based on DAS-ELISA, 100% of the survived plantlets by meristem culture were virus-free. DMV infected stem segments with two axillary buds were treated at three different levels of electric currents (15, 25 and 35 mA) and two time courses (10 and 20 min) in an electrophoresis tank and cultured on MS medium. The treatment of 35 mA for 20 min was the best for viral elimination from diseased dahlias by 85% followed by 25 mA for 20 min by 70%. Acyclovir and salicylic acid were the antivirals were used to eliminate DMV from the infected dahlias. For chemotherapy, nodes were cultured on MS media supplemented with antivirals at 0, 10, 20, 30, 40 and 50 mg/l for 20 and 30 days. The highest concentration of acyclovir (50 mg/l) for 30 days were the best treatments as its highest effectiveness on elimination ability (90.67%) but gave only 49% for plantlet survival. Whereas 40 mg/l acyclovir for 30 days gave elimination ability (80%) and plantlet survival (89.67%). The therapy containing salicylic acid at 40 mg/l for 30 days was the best as its highest effectiveness on DMV elimination ability (100%). But the concentration of salicylic acid at 30 mg/l for 30 days was the best treatment (chemotherapy efficiency= 49.21) as well as DMV elimination ability (75.33%) and plant survival (65.33%). The in vitro developed dahlia plantlets were tested for DMV-freeness using DAS-ELISA. The using therapies (electrotherapy and chemotherapy) can play a good role in virus elimination because they need the minimum period of time to regenerate fully developed healthy plants as long as 2-3 months.

scholarly journals Pengaruh PVP dan DIECA terhadap Regenerasi Meristem Tebu

2016 ◽  
Vol 7 (1) ◽  
pp. 9
Author(s):  
Ika Roostika ◽  
Rara Puspita Dewi Lima Wati ◽  
Deden Sukmadjaja
Keyword(s):  
Survival Rate ◽  
High Risk ◽  
Sugar Cane ◽  
Plant Material ◽  
Combined Treatment ◽  
Saccharum Officinarum ◽  
Meristem Culture ◽  
Regeneration Capacity ◽  
Virus Elimination ◽  
Regeneration Rate

<p>Tebu (Saccharum officinarum L.) merupakan tanaman yang diperbanyak secara vegetatif sehingga berisiko besar akan terjadinya akumulasi virus di dalam jaringan tanaman. Kultur meristem merupakan salah satu teknik eliminasi virus yang umum digunakan, namun seringkali meristem memiliki daya hidup dan daya re-generasi yang rendah. Salah satu penyebabnya adalah karena akumulasi senyawa fenol. Akumulasi senyawa tersebut dapat direduksi melalui penggunaan senyawa adsorben dan antioksidan. Penelitian ini bertujuan un-tuk mengetahui pengaruh polyvinylpyrrolidone (PVP) dan diethyldithiocarbamate sodium (DIECA) terhadap regenerasi meristem tebu. Bahan tanaman yang digunakan adalah tebu PS864. Eksplan yang digunakan adalah meristem dengan 1–2 primordia daun yang diisolasi di bawah mikroskop. Perlakuan meliputi PVP (100 dan 300 mg/l) dan DIECA (0 dan 20 mg/l) serta kombinasi antara kedua zat tersebut, dengan 3 ulang-an (botol) dan setiap botol terdiri atas 3 meristem. Hasil penelitian menunjukkan bahwa peningkatan kon-sentrasi PVP atau kombinasi perlakuan PVP dan DIECA dapat meningkatkan persentase eksplan hidup, daya regenerasi, dan jumlah tunas. Kombinasi perlakuan PVP 300 mg/l dan DIECA 20 mg/l merupakan perlakuan terbaik karena persentase hidup dan daya regenerasi eksplan yang paling tinggi (100%) dengan jumlah tu-nas 3,8 tunas/eksplan.</p><p> </p><p>Being vegetatively propagated, sugar cane faces a high risk of virus accumulation. Meristem culture is one method that usually applied for virus elimination. However, it often has low survival and regeneration rate due to an accumulation of phenolic compounds. Accumulation of those compounds can be reduced by apply adsorbent antioxidant. This research aimed at evaluating the effect of PVP and DIECA on the regeneration capacity of meristem. The plant material was sugar cane PS864. Meristems with 1─2 primoridial leaves were used as the explants and isolated under microscope. The PVP (100−300 mg/l) and DIECA (0− 20 mg/l), or combined treatment of both antioxidants were used as treatments. Each treatment was replicated 3 times (bottles), and each bottle contained 3 meristems. The result showed that the higher concentration of PVP or combined treatment of PVP and DIECA could increase the percentage of survival, regeneration rate, and number of shoot. The combined treatment of 300 mg/l PVP, and 20 mg/l DIECA produced the highest level of survival rate (100%) which yielded 3.8 shoots/explants.</p>

Meristem Culture for Virus Elimination

1995 ◽  
pp. 117-128 ◽  
Author(s):  
Steven A. Slack ◽  
Laura A. Tufford
Keyword(s):  
Meristem Culture ◽  
Virus Elimination

Meristem Culture for Virus Elimination and Peanut Interspecific Hybrid Preservation

Crop Science ◽  
1997 ◽  
Vol 37 (2) ◽  
pp. 591-594 ◽  
Author(s):  
J. B. Morris ◽  
S. Dunn ◽  
D. L. Pinnow ◽  
M. S. Hopkins ◽  
R. N. Pittman
Keyword(s):  
Interspecific Hybrid ◽  
Meristem Culture ◽  
Virus Elimination

scholarly journals Efficient virus elimination for apple dwarfing rootstock M.9 and M.26 via thermotherapy, ribavirin and apical meristem culture

2019 ◽  
Vol 46 (3) ◽  
pp. 228-235
Author(s):  
Young Hee Kwon ◽  
Joung Kwan Lee ◽  
Hee Kyu Kim ◽  
Kyung Ok Kim ◽  
Jae Seong Park ◽  
...  
Keyword(s):  
Apical Meristem ◽  
Meristem Culture ◽  
Virus Elimination

scholarly journals Anti-severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) potency of Mefloquine as an entry inhibitor in vitro

2020 ◽  
Author(s):  
Kaho Shionoya ◽  
Masako Yamasaki ◽  
Shoya Iwanami ◽  
Yusuke Ito ◽  
Shuetsu Fukushi ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Viral Entry ◽  
Combined Treatment ◽  
Entry Inhibitor ◽  
Economic Damage ◽  
Virus Elimination ◽  
Viral Attachment ◽  
Approved Drugs ◽  
Treatment Dosage

AbstractCoronavirus disease 2019 (COVID-19) has caused serious public health, social, and economic damage worldwide and effective drugs that prevent or cure COVID-19 are urgently needed. Approved drugs including Hydroxychloroquine, Remdesivir or Interferon were reported to inhibit the infection or propagation of severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), however, their clinical efficacies have not yet been well demonstrated. To identify drugs with higher antiviral potency, we screened approved anti-parasitic/anti-protozoal drugs and identified an anti-malarial drug, Mefloquine, which showed the highest anti-SARS-CoV-2 activity among the tested compounds. Mefloquine showed higher anti-SARS-CoV-2 activity than Hydroxychloroquine in VeroE6/TMPRSS2 and Calu-3 cells, with IC50 = 1.28 μM, IC90 = 2.31 μM, and IC99 = 4.39 μM in VeroE6/TMPRSS2 cells. Mefloquine inhibited viral entry after viral attachment to the target cell. Combined treatment with Mefloquine and Nelfinavir, a replication inhibitor, showed synergistic antiviral activity. Our mathematical modeling based on the drug concentration in the lung predicted that Mefloquine administration at a standard treatment dosage could decline viral dynamics in patients, reduce cumulative viral load to 7% and shorten the time until virus elimination by 6.1 days. These data cumulatively underscore Mefloquine as an anti-SARS-CoV-2 entry inhibitor.

scholarly journals Mefloquine, a Potent Anti-severe Acute Respiratory Syndrome-Related Coronavirus 2 (SARS-CoV-2) Drug as an Entry Inhibitor in vitro

2021 ◽  
Vol 12 ◽  
Author(s):  
Kaho Shionoya ◽  
Masako Yamasaki ◽  
Shoya Iwanami ◽  
Yusuke Ito ◽  
Shuetsu Fukushi ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Viral Entry ◽  
Combined Treatment ◽  
Entry Inhibitor ◽  
Economic Damage ◽  
Virus Elimination ◽  
Viral Attachment ◽  
Approved Drugs ◽  
Treatment Dosage

Coronavirus disease 2019 (COVID-19) has caused serious public health, social, and economic damage worldwide and effective drugs that prevent or cure COVID-19 are urgently needed. Approved drugs including Hydroxychloroquine, Remdesivir or Interferon were reported to inhibit the infection or propagation of severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), however, their clinical efficacies have not yet been well demonstrated. To identify drugs with higher antiviral potency, we screened approved anti-parasitic/anti-protozoal drugs and identified an anti-malarial drug, Mefloquine, which showed the highest anti-SARS-CoV-2 activity among the tested compounds. Mefloquine showed higher anti-SARS-CoV-2 activity than Hydroxychloroquine in VeroE6/TMPRSS2 and Calu-3 cells, with IC50 = 1.28 μM, IC90 = 2.31 μM, and IC99 = 4.39 μM in VeroE6/TMPRSS2 cells. Mefloquine inhibited viral entry after viral attachment to the target cell. Combined treatment with Mefloquine and Nelfinavir, a replication inhibitor, showed synergistic antiviral activity. Our mathematical modeling based on the drug concentration in the lung predicted that Mefloquine administration at a standard treatment dosage could decline viral dynamics in patients, reduce cumulative viral load to 7% and shorten the time until virus elimination by 6.1 days. These data cumulatively underscore Mefloquine as an anti-SARS-CoV-2 entry inhibitor.

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