Effect of Antivirals on the Elimination of Lily Symptomless Virus in Lilium Oriental Hybrid `Casa Blanca'
This study was conducted to find out the effect of antivirals during tissue culture for lily symptomless virus (LSV) free stock production of Oriental lily. Scales and bulblets of LSV-infected Lilium Oriental hybrids `Casa Blanca' were used as plant materials. Scales and bulblets were cultured in Murashige and Skoog (MS, 1962) medium added 20 μM of virazole, and 0, 20, 40, and 80 μM of azauracil or thiouracil for 12 weeks prior to LSV detection to examine the effect of antivirals. The survival rate in scaling was observed to be lower than bulblet culture in vitro and higher concentration of azauracil and thiouracil also effected on lower survival rate. The enlargement of bulblet tended to be promoted in low concentration without regard to kinds of antivirals, and especially most effective in 20 μM of azauracil. Elimenation rate of LSV was 0% in control, on the contrary, antivirals treatment which resulted in complete elimination of LSV.