scholarly journals Direct Shoot Organogenesis in Murraya paniculata (L.) Jack: A Prerequisite for Genetic Transformation

HortScience ◽  
2013 ◽  
Vol 48 (7) ◽  
pp. 938-941 ◽  
Author(s):  
Xiuli Shen ◽  
Vladimir Orbović ◽  
Manjul Dutt ◽  
William S. Castle ◽  
Frederick G. Gmitter
Keyword(s):  
Genetic Transformation ◽  
Shoot Organogenesis ◽  
In Vitro Regeneration ◽  
Ms Medium ◽  
Shoot Induction ◽  
Regeneration System ◽  
Murraya Paniculata ◽  
Direct Shoot Organogenesis ◽  
Direct Shoot

An efficient in vitro regeneration system through direct shoot organogenesis was established for Murraya paniculata (L.) Jack (Orange Jessamine). Epicotyls, leaves, roots, and cotyledons from in vitro-germinated seedlings and several plant growth regulators (PGRs) were evaluated for their effects on plant regeneration. Longitudinally cut epicotyl segments were observed to be the optimal explants followed by uncut epicotyls (not longitudinally cut). Roots, leaves, and cotyledons were not suitable as explants as a result of little or no shoot induction. Adventitious shoot induction was enhanced by the addition of 6-benzyladenine (BA). The highest percentage of shoot induction (87%) and the greatest number of shoots per explant (12.7) occurred on Murashige and Skoog (MS) medium supplemented with 15 μM BA from longitudinally cut epicotyls followed by 5.2 shoots per explant from uncut epicotyls. Optimal concentration of gibberellic acid (GA3) for shoot elongation was observed to be 15 μM. Eighty-five percent of the regenerated shoots produced roots with an average of three roots per shoot on MS medium supplemented with 5 μM indole-3-butyric acid (IBA). Our protocol for direct shoot organogenesis can potentially lead to the development of a robust method for production of transgenic plants of M. paniculata through Agrobacterium-mediated genetic transformation.

scholarly journals Direct Shoot Organogenesis of Medicago sativa

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 628f-628
Author(s):  
Guochen Yang ◽  
Marihelen Kamp-Glass
Keyword(s):  
Genetic Transformation ◽  
Shoot Regeneration ◽  
Shoot Organogenesis ◽  
Multiple Shoots ◽  
Direct Shoot Regeneration ◽  
Callus Initiation ◽  
Direct Shoot Organogenesis ◽  
Direct Shoot ◽  
In Vitro Shoot Regeneration

An efficient and reliable protocol of in vitro shoot regeneration must be first established to have a successful genetic transformation. As a member of legume family, alfalfa is very difficult for direct shoot regeneration. There is no published information on direct shoot organogenesis, although success has been well documented on embryogenesis, which must go through callus stage. Different plant growth regulators at various concentrations were evaluated for callus initiation, development, and direct shoot regeneration. Multiple shoots were produced directly from each individual explant. This will provide an efficient means for production of transgenic alfalfa plants. Therefore, genetic transformation of Medicago germplasm will be significantly expedited.

scholarly journals High Efficiency Direct Shoot Organogenesis from Leaf Segments ofAerva lanata(L.) Juss. Ex Schult by Using Thidiazuron

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
K. Varutharaju ◽  
C. Soundar Raju ◽  
C. Thilip ◽  
A. Aslam ◽  
A. Shajahan
Keyword(s):  
Shoot Organogenesis ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Naphthaleneacetic Acid ◽  
Scale Production ◽  
Ms Medium ◽  
Leaf Segments ◽  
Direct Shoot Organogenesis ◽  
Direct Shoot

An efficient protocol for direct shoot organogenesis has been developed for the medicinal plantAerva lanata(L.) Juss. ex Schult. Regeneration was achieved from leaf segments of 20 days oldin vitroplantlets raised on Murashige and Skoog (MS) medium containing 0.25–2.0 mg L−1thiadiazuron (TDZ), 3% sucrose, and 0.8% agar. After 21 days of culture incubation, maximum number of shoot organogenesis (23.6 ± 0.16) was obtained on medium containing 1.0 mg L−1TDZ. The shoots were able to producein vitroflowers on medium containing 1.0 mg L−1TDZ in combination with 0.25–0.5 mg L−1  α-naphthaleneacetic acid (NAA). Histological observation showed that the epidermal cells of the leaf explants exhibited continuous cell division led to formation of numerous dome shaped meristematic protrusions and subsequently developed into adventitious shoots. Upon transfer of shootlets to half strength MS medium containing 1.0 mg L−1indole-3-butyric acid (IBA), around 86% of the regenerated shoots formed roots and plantlets. Rooted plants were hardened and successfully established in the soil at the survival rate of 92%. The regeneration protocol developed in this study provides an important method of micropropagation of this plant. Furthermore, this protocol may be used for a large scale production of its medicinally active compounds and genetic transformations for further improvement.

scholarly journals (405) Regeneration of Venus Fly Trap (Dionaea muscipula Ellis) from Leaf Culture

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1062F-1063
Author(s):  
Khalid M. Ahmad ◽  
Syed M. A. Zobayed ◽  
Praveen K. Saxena ◽  
David M. Hunter
Keyword(s):  
Somatic Embryogenesis ◽  
Shoot Organogenesis ◽  
In Vitro Regeneration ◽  
Leaf Disc ◽  
Leaf Explants ◽  
Carnivorous Plant ◽  
In Vitro Techniques ◽  
Direct Shoot Organogenesis ◽  
Direct Shoot

Dionaeamuscipula Ellis commonly known as Venus fly trap is an important carnivorous plant with medicinal importance. It contains certain secondary metabolites like naphthoquinones and is used in anti-aid and anti-cancer drugs and other medicines like Cornivora. Increasing interest and use as an ornamental and medicinal plant, and dietary supplement have put it in an endangered state. Development of in vitro techniques for the preservation of germplasm that is on the brink of extinction is highly demanded. A regeneration protocol for the multiplication and micropropagation of Dionaeamuscipla Ellis was established. In vitro regeneration potential of leaf explants in different concentrations and combinations of plant growth substances was investigated in this study. Seeds were grown and leaf disc explants were excised and cultured under aseptic conditions on nutritional medium containing half strength Murashige and Skoog (MS) mix with combinations of 1.0–20.0 μm BA, 2.5.0 μm IBA, 1.0–10.0 μm 2iP and 0.1–0.5μm TDZ. The cultures were kept in growth cabinet with cool white light (40–60 μmol·m-2·s-1) under 16-h photoperiod. Regeneration was recorded after 60 days with the intervals of 15 days based on the degree of shoot organogenesis and somatic embryogenesis. 1/2 MS + 0.1 TDZ appeared to be efficient for somatic embryogenesis and simple MS for direct shoot organogenesis. 1/2 MS combined with 2iP appeared to be efficient for regeneration either by direct shoot organogenesis or by somatic embryogenesis. Plants were rooted well in Cape Cundew medium. These investigations will aid in the development of a model system for clonal mass propagation and in vitro regeneration of Dionaeamuscipla Ellis.

scholarly journals In vitro regeneration through direct shoot organogenesis in Honey Orange (Citrus tangerina)

2014 ◽  
Vol 31 (4) ◽  
pp. 341-344 ◽  
Author(s):  
Yin Yin Nwe ◽  
Khin Thida Myint ◽  
Yuya Mochizuki ◽  
Mehran Vazirzanjani ◽  
Kouji Okayasu ◽  
...  
Keyword(s):  
Shoot Organogenesis ◽  
In Vitro Regeneration ◽  
Direct Shoot Organogenesis ◽  
Direct Shoot

scholarly journals Induction of Direct Shoot Organogenesis from Shoot Tip Explants of an Ornamental Aquatic Plant, Cryptocoryne wendtii

2018 ◽  
Vol 17 (4) ◽  
pp. 293-302
Author(s):  
Sutha KLAOCHEED ◽  
Wanna JEHSU ◽  
Wanwilai CHOOJUN ◽  
Kanchit THAMMASIRI ◽  
Somporn PRASERTSONGSKUN ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
Aquatic Plant ◽  
Shoot Organogenesis ◽  
Shoot Tip ◽  
Scale Production ◽  
Ms Medium ◽  
Shoot Tip Explants ◽  
Direct Shoot Organogenesis ◽  
Direct Shoot

Cryptocoryne wendtii is an important amphibious species with a wide range of foliage colors. Although it has a high market demand, the natural propagation of its aquatic species is limited due to the limited production on the number of plants with a long cultivation period, disease, and the requirement for a large space for propagation. Thus, we studied the effects of the plant growth regulators and their concentrations on the induction of direct shoot organogenesis from shoot tip explants of Cryptocoryne wendtii. The shoot tips were sterilized on its surface using 8 % Clorox® (5.25 % sodium hypochlorite, NaOCl) for 15 min followed by rinsing them three times with sterile distilled water. They were again sterilized on the surface for another 4 % Clorox® (5.25 % sodium hypochlorite, NaOCl) for 5 min. Cytokinins played a crucial role in direct shoot organogenesis. Direct shoot organogenesis from shoot tip explants was promoted by incubating these explants on Murashige and Skoog (MS) medium [1] in the presence of two different cytokinins [6-benzyl-aminopurine (BAP) or Kinetin (Kin)], each provided at four different levels. Direct shoot organogenesis was induced in both explants. Shooting occurred 100 % from in vitro shoot tip explants, which was cultured on MS medium and supplemented with 3.0 mg/l BAP. This was significantly different from the other treatments with the highest number of 16.20 shoots per explant and number of leaves at 72.40 leaves per explant after 60 days of culture. Individual shoots, aseptically excised, which produced normal roots within 45 days on the MS medium supplemented with α-Naphthaleneacetic acid (NAA). The highest number of roots per shoot and the longest roots were obtained on MS medium supplemented with 1.0 mg/l NAA (100 % rooting, which was an average of 36 roots per plantlet and root length at 26.02 mm). Rooted plantlets were successfully hardened and established in pots containing a mixture of organic soil and sand (1:1) overlaid with tap water under greenhouse conditions at 90 % survival. This complete study has successfully outlined a rapid, high frequency direct shoot organogenesis induction of an ornamental aquatic plant, Cryptocoryne wendtii from shoot tip explants inclusive of shoot proliferation, rooting and acclimatization. The present in vitro propagation protocol would facilitate an alternative method for rapid, large-scale production and germplasm preservation of this important endangered species C. wendtii.

scholarly journals In vitro Regeneration of Dalbergia sissoo Roxb. and the Potential for Genetic Transformation

2012 ◽  
Vol 40 (2) ◽  
pp. 140 ◽  
Author(s):  
Hafiz Mamoon REHMAN ◽  
Iqrar Ahmad RANA ◽  
Siddra IJAZ ◽  
Ghulam MUSTAFA ◽  
Faiz Ahmad JOYIA ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Genetic Transformation ◽  
Callus Induction ◽  
In Vitro Regeneration ◽  
Induction Medium ◽  
Native Forest ◽  
Ms Medium ◽  
Dalbergia Sissoo ◽  
Callus Induction Medium

Dalbergia sissoo Roxb. ex DC. (Sissoo) is a native forest tree species in Pakistan. Many ecological and economical uses are associated with this premier timber species, but dieback disease is of major concern. The objective of this study was to develop a protocol for in vitro regeneration of Sissoo that could serve as target material for genetic transformation, in order to improve this species. Callus formation and plantlet regeneration was achieved by culturing cotyledons, immature seeds, and mature embryos on a modified Murashige and Skoog (1962) (MS) medium supplemented with plant growth regulators. Callus induction medium containing 2.71 ?M 2, 4-dichlorophenoxyacetic acid (2,4-D) and 0.93 ?M kinetin produced better callus on all explants tested compared to other treatments, such as 8.88 ?M 6-benzylaminopurine (BA) and 2.69 ?M ?-naphthalene acetic acid (NAA), or 2.71 ?M 2, 4-D and 2.69 ?M NAA. Shoot regeneration was best on MS medium containing 1.4 ?M NAA and 8.88 ?M BA compared to other treatments, such as 1.4 ?M NAA and 9.9 ?M kinetin, or 2.86 ?M indole-3-acetic acid and 8.88 ?M BA. Murashige and Skoog medium containing 1.4 NAA ?M and 8.88 ?M BA was better in general for regeneration regardless of callus induction medium and the type of explant used. Rooting was best on half-strength MS medium with 7.35 ?M indole-3-butyric acid. Regenerated plantlets were acclimatized for plantation in the field. Preliminary genetic transformation potential of D. sissoo was evaluated by particle bombardment of callus explants with a pUbiGus vector. The bombarded tissue showed transient Gus activity 1week after bombardment. Transformation of this woody tree is possible provided excellent regeneration protocols. The best combination for regeneration explained in this study is one of such protocols.

scholarly journals In vitro Regeneration and Rapid Multiplication of Dendrobium bensoniae, an Indigenous Ornamental Orchid

2017 ◽  
Vol 14 (2) ◽  
pp. 24-31 ◽  
Author(s):  
S S Riva ◽  
A Islam ◽  
M E Hoque
Keyword(s):  
Shoot Regeneration ◽  
In Vitro Regeneration ◽  
Ms Medium ◽  
Shoot Induction ◽  
Rapid Multiplication ◽  
Number Of Shoots

An experiment was conducted on in vitro regeneration and multiplication of Dendrobium bensoniae. Different concentrations of BA and IBA alone or combination of both hormones were used as treatment for regeneration.  It was revealed that shoot regeneration from node was the best at 2.0 mg/l BA supplemented to MS medium. It gave better responses than all other concentrations and combinations of BA and BA+IBA, used in the present study. The highest number of shoots and leaves were found when 1.0 mg/l BA with 1.5 mg/l IBA was supplemented into MS medium.  For rooting, 0.5 mg/l BA with 1.0 mg/l IBA was found to be the most effective. The well-rooted plantlets were successfully acclimatized under 70-80% humidity and planted in pots and transferred to the shade house for establishment. Around 85% of plantlets survived in the field. From the present result, it may be recommended that MS medium supplemented with 2.0 mg/l BA may be used for rapid shoot induction and regeneration of D. bensoniae.The Agriculturists 2016; 14(2) 24-31

Sign in / Sign up

Export Citation Format

Share Document