Decreased serotonin content and release in the ventral hippocampus of prenatally stressed male rats in response to forced swim test

Previously we revealed for the first time pain response exacerbation caused by inflammation in rats born to dams exposed to stress during pregnancy (prenatal stress). The present study is devoted to tinvestigation of prenatal stress effects on psychoemotional and tonic pain reactions in rat pups during the individual development period that is characterized with a dramatic reduction of the brain serotonin level. Effects of maternal buspirone before stress during pregnancy on functional indices of psychoemotional and tonic pain systems in the offspring were also investigated. Prenatal stress increased the number of pain patterns (flexing + shaking) during different phases of the time-course of formalin-induced pain in females and males to a greater extent in males. Prenatlly stressed rat pups of both sexes failed to show reliable changes in the index of psychoemotional behavior in the forced swim test. With the aim to decrease pain response exacerbation found in prenatally stressed offspring, pregnant dams were exposed to chronic injections of serotoninergic anxiolytic and antidepressant buspirone which is an agonist of 5-HT1A receptors; prenatal effect of buspirone on psychoemotional behavior in prenatally stressed rat pups was also evaluated. Maternal buspirone normalized pain behavior and decreased considerably the time of immobility, the index of depressive behavior in the forced swim test. The present results indicate analgesic and antidepressive effects of maternal buspirone in prenatally stressed 10-day old rat pups and demonstrate sexual dimorphism in effects of prenatal stress on the time-course of formalin-induced pain. Differences in effects of prenatal influences on pain respone during the interphase in males and females indicate earlier maturation of the descending serotonergic inhibitory system of afferent pain signals modulation in males than in females and demonstrate that 5-HT1A receptors are involved in this process.

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Study of the Role of Dopamine Receptors in Streptozotocin-Induced Depressive-Like Behavior Using the Forced Swim Test Model

Galen Medical Journal ◽

10.31661/gmj.v7i0.954 ◽

2017 ◽

Vol 7 ◽

pp. e954

Author(s):

Afshin Roostaei ◽

Gholamhassan Vaezi ◽

Mohammad Nasehi ◽

Ali Haeri-Rohani ◽

Mohammad-Reza Zarrindast

Keyword(s):

Receptor Antagonist ◽

Dopamine Receptors ◽

Dopamine Receptor ◽

Forced Swim Test ◽

Male Rats ◽

Test Model ◽

Dopamine Receptor Antagonist ◽

Swim Test ◽

Forced Swim

Background: Diabetes is one of the most common endocrine diseases characterized by hyperglycemia. It is caused by an absolute or relative insulin deficiency or an insulin function deficiency. It is one of the major risk factors of depression, with the rate of depression in diabetic patients amounting to as high as 30%. This study examined the role of dopamine receptors in streptozotocin (STZ)-induced depressive-like behavior using the forced swim test (FST). Materials and Methods: This study was performed on 56 Wistar male rats. STZ at doses of 30 and 60 mg/kg body weight was administered via intraperitoneal (IP) route to induce diabetes and depression in rats. Thereafter, by using halobenzazepine (SCH23390) (D1 dopamine receptor antagonist) and sulpiride (D2 receptor dopamine receptor antagonist), the role of dopamine receptors in STZ-induced depression was studied. The one-way analysis of variance technique, Tukey’s range test, and t-test were used to analyze the data. The P-value less than 0.05 was regarded as statistically significant. Results: Our study showed that STZ at doses of 30 and 60 mg/kg, two weeks after injection, caused prolonged immobility in FST, indicating depressive-like behavior (P<0.05 and P<0.01, respectively). SCH23390 (0.001 mg/mL/kg) and sulpiride (0.1mg/mL/kg) did not change the variables of depression in animals that received STZ (at doses of 30 and 60 mg/mL/kg) two weeks before (P>0.05). Conclusion: According to our study, STZ has a depressive-like behavior two weeks after injection, and dopamine receptors do not play a role in depression associated with STZ use. [GMJ.2018;7:e954]

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Dapagliflozin attenuates depressive-like behavior of male rats in the forced swim test

10.26226/morressier.5b681761b56e9b005965c395 ◽

2018 ◽

Author(s):

Cam Muhammet Emin ◽

Ayşe Nur Hazar-Yavuz ◽

Yildiz Sila ◽

Rumeysa Keles ◽

Ertaş Büşra

Keyword(s):

Forced Swim Test ◽

Male Rats ◽

Swim Test ◽

Forced Swim

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Investigation of Diagnostic Proteins by 2D Electrophoresis in Major De-pression Model Induced by Forced Swim Test in Rats.

Protein and Peptide Letters ◽

10.2174/0929866527999200729183729 ◽

2020 ◽

Vol 27 ◽

Author(s):

Tuğçe Duman ◽

Enes Akyüz ◽

Huri Bulut ◽

Abdurrahim Koçyiğit ◽

Ahmet Tülek ◽

...

Keyword(s):

Forced Swim Test ◽

Diagnostic System ◽

Economic Consequences ◽

Male Rats ◽

2D Electrophoresis ◽

Serum Samples ◽

Swim Test ◽

Forced Swim ◽

Potential Biomarker ◽

The Matrix

Background: Aside from its pervasiveness, whereby it affects as much as 20% of the world's population, depression continues to be one of the most crucial psychiatric problems due to the loss of power it causes by disrupting daily life functioning, containing economic consequences, and having a high suicidal tendency. Major depression (MD) is a systemic and multifactorial disorder involving complex interactions between genetic predisposition and disturbances of various molecular pathways. Objectives: In our current study, we aimed to identify the proteins obtained from serum samples that change during depression with the MD model. Methods : The MD model was applied through the forced swim test in rats. 14 Winstar Albino male rats were divided into two equal groupsas follows: depression and control groups. Serum samples were separated by chromatographic methods and then compared with two-dimensional (2D) electrophoresis. Results: A total of 9 potential diagnostic protein sequences were identified, which were distinguished with computer soft-ware. During the last phase of the study,the Matrix-Assisted Laser Desorption/Ionization –Time of Flight (MALDI-TOF) analysis, the previous expression sequences identified among the groups were determinedand classified. By comparing pro-tein expressions, it was concluded that 9 different points could be used together as a potential biomarker. Conclusion: Results can help us identify a new diagnostic system that can be used to diagnose MD.

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