Characterization of Resistance to Soybean mosaic virus in Diverse Soybean Germplasm

Crop Science ◽  
2005 ◽  
Vol 45 (6) ◽  
pp. 2503-2509 ◽  
Author(s):  
C. Zheng ◽  
P. Chen ◽  
R. Gergerich
Keyword(s):  
Mosaic Virus ◽  
Soybean Mosaic Virus ◽  
Soybean Germplasm

Characterization of Soybean mosaic virus resistance derived from inverted repeat-SMV-HC-Pro genes in multiple soybean cultivars

2015 ◽  
Vol 128 (8) ◽  
pp. 1489-1505 ◽  
Author(s):  
Le Gao ◽  
Xueni Ding ◽  
Kai Li ◽  
Wenlin Liao ◽  
Yongkun Zhong ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Virus Resistance ◽  
Inverted Repeat ◽  
Soybean Mosaic Virus ◽  
Soybean Cultivars

scholarly journals Analysis of Soybean mosaic virus genetic diversity in Iran allows the characterization of a new mutation resulting in overcoming Rsv4-resistance

2013 ◽  
Vol 94 (11) ◽  
pp. 2557-2568 ◽  
Author(s):  
Akbar Ahangaran ◽  
Mina Koohi Habibi ◽  
Gholam-Hossein Mosahebi Mohammadi ◽  
Stephan Winter ◽  
Fernando García-Arenal
Keyword(s):  
Genetic Diversity ◽  
Amino Acid ◽  
Mosaic Virus ◽  
Phylogenetic Analyses ◽  
Soybean Mosaic Virus ◽  
Biologically Active ◽  
Site Directed Mutagenesis ◽  
Sequence Comparisons ◽  
Strong Negative Selection

The genetic variation and population structure of Soybean mosaic virus (SMV) in Iran was analysed through the characterization of a set of isolates collected in the soybean-growing provinces of Iran. The partial nucleotide sequence of these isolates showed a single, undifferentiated population with low genetic diversity, highly differentiated from other SMV world populations. These traits are compatible with a population bottleneck associated with the recent introduction of SMV in Iran. Phylogenetic analyses suggest that SMV was introduced into Iran from East Asia, with at least three introduction events. The limited genetic diversification of SMV in Iran may be explained by strong negative selection in most viral genes eliminating the majority of mutations, together with recombination purging deleterious mutations. The pathogenicity of Iranian SMV isolates was typified on a set of soybean differential lines either susceptible or carrying different resistance genes or alleles to SMV. Two pathotypes were distinguished according to the ability to overcome Rsv4 resistance in line V94-5152. Amino acid sequence comparisons of virulent and avirulent isolates on V94-5152 (Rsv4), plus site-directed mutagenesis in a biologically active cDNA clone, identified mutation S1053N in the P3 protein as the determinant for virulence on V94-5152. Codon 1053 was shown to be under positive selection, and S1053N-determined Rsv4-virulence occurred in isolates with different genealogies. The V94-5152 (Rsv4)-virulence determinant in Iranian isolates maps into a different amino acid position in the P3 protein than those previously reported, indicating different evolutionary pathways towards resistance breaking that might be conditioned by sequence context.

Molecular characterization of NBS–LRR genes in the soybean Rsv3 locus reveals several divergent alleles that likely confer resistance to the soybean mosaic virus

2017 ◽  
Vol 131 (2) ◽  
pp. 253-265 ◽  
Author(s):  
Fang-Fang Ma ◽  
Mian Wu ◽  
Ying-Na Liu ◽  
Xue-Ying Feng ◽  
Xun-Zong Wu ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Molecular Characterization ◽  
Soybean Mosaic Virus

scholarly journals Identification of a New Genetic Clade of Cowpea Mild Mottle Virus and Characterization of Its Interaction With Soybean Mosaic Virus in Co-infected Soybean

2021 ◽  
Vol 12 ◽  
Author(s):  
Zhongyan Wei ◽  
Chenyang Mao ◽  
Chong Jiang ◽  
Hehong Zhang ◽  
Jianping Chen ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
High Throughput Sequencing ◽  
Soybean Mosaic Virus ◽  
Mottle Virus ◽  
Genome Sequences ◽  
Genomic Rnas ◽  
Disease Symptoms ◽  
Three Samples ◽  
Soybean Plants

Cowpea mild mottle virus (CPMMV; genus Carlavirus) can be a destructive pathogen of soybean but there is little information about its distribution on soybean in China. Here, we collected soybean plants with virus-like symptoms from 11 fields widely scattered within China, and used high-throughput sequencing to determine their virome. Most samples (8/11) were co-infected by the well-studied potyvirus soybean mosaic virus (SMV) and CPMMV, and the remaining three samples were singly infected with CPMMV. The near-complete genome sequences of the 11 CPMMV isolates were determined and phylogenetic analysis showed that they constituted a new genetic clade. One recombination event was detected among the CPMMV sequences, and the isolate CPMMV_JL_CC was identified as recombinant. In mechanical inoculation assays, co-infection by CPMMV and SMV resulted in an enhancement of disease symptoms, but decreased the expression level of the genomic RNAs and CP of CPMMV, without significantly affecting SMV accumulation. The interaction between these viruses needs further investigation.

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