High Levels of Heritable Resistance to Potato Leafroll Virus (PLRV) in Solanum tuberosum subsp. andigena

Crop Science ◽  
2007 ◽  
Vol 47 (3) ◽  
pp. 1091-1103 ◽  
Author(s):  
E. Mihovilovich ◽  
L. Alarcón ◽  
A. L. Pérez ◽  
J. Alvarado ◽  
C. Arellano ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Potato Leafroll Virus ◽  
Leafroll Virus

scholarly journals Host-Dependent Requirement for the Potato leafroll virus 17-kDa Protein in Virus Movement

2002 ◽  
Vol 15 (10) ◽  
pp. 1086-1094 ◽  
Author(s):  
Lawrence Lee ◽  
Peter Palukaitis ◽  
Stewart M. Gray
Keyword(s):  
Amino Acids ◽  
Solanum Tuberosum ◽  
Nicotiana Benthamiana ◽  
Systemic Infection ◽  
Potato Leafroll Virus ◽  
Virus Movement ◽  
Wild Type ◽  
Mature Leaves ◽  
Leafroll Virus ◽  
Virus Distribution

The requirement for the 17-kDa protein (P17) of Potato leafroll virus (PLRV) in virus movement was investigated in four plant species: potato (Solanum tuberosum), Physalis floridana, Nicotiana benthamiana, and N. clevelandii. Two PLRV P17 mutants were characterized, one that does not translate the P17 and another that expresses a P17 missing the first four amino acids. The P17 mutants were able to replicate and accumulate in agroinoculated leaves of potato and P. floridana, but they were unable to move into vascular tissues and initiate a systemic infection in these plants. In contrast, the P17 mutants were able to spread systemically from inoculated leaves in both Nicotiana spp., although the efficiency of infection was reduced relative to wild-type PLRV. Examination of virus distribution in N. benthamiana plants using tissue immunoblotting techniques revealed that the wild-type PLRV and P17 mutants followed a similar movement pathway out of the inoculated leaves. Virus first moved upward to the apical tissues and then downward. The P17 mutants, however, infected fewer phloem-associated cells, were slower than wild-type PLRV in moving out of the inoculated tissue and into apical tissues, and were unable to infect any mature leaves present on the plant at the time of inoculation.

scholarly journals Temperature and CO2 Level Influence Potato leafroll virus Infection in Solanum tuberosum

2017 ◽  
Vol 33 (5) ◽  
pp. 522-527 ◽  
Author(s):  
Bong Nam Chung ◽  
Sang Wook Koh ◽  
Kyung San Choi ◽  
Jae Ho Joa ◽  
Chun Hwan Kim ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Virus Infection ◽  
Potato Leafroll Virus ◽  
Co2 Level ◽  
Leafroll Virus

scholarly journals Introgression of the Rl adg allele of resistance to potato leafroll virus in Solanum tuberosum L.

2017 ◽  
Vol 17 (3) ◽  
pp. 242-249 ◽  
Author(s):  
Otávio Luiz Gomes Carneiro ◽  
Silvia Regina Rodrigues de Paula Ribeiro ◽  
Carolina Mariane Moreira ◽  
Marcio Lisboa Guedes ◽  
Danilo Hottis Lyra ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Potato Leafroll Virus ◽  
Leafroll Virus

scholarly journals Detection and sequencing of Potato virus Y (PVY) and Potato leafroll virus (PLRV) in a volunteer plant of Solanum tuberosum L. cv. Diacol-Capiro

2017 ◽  
Vol 66 (4) ◽  
pp. 625-632 ◽  
Author(s):  
Héctor Camilo Medina Cárdenas ◽  
Pablo Andrés Gutiérrez Sánchez ◽  
Mauricio Alejandro Marín Montoya
Keyword(s):  
Solanum Tuberosum ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Solanum Tuberosum L ◽  
Potato Leafroll Virus ◽  
Leafroll Virus ◽  
Volunteer Plant

scholarly journals Diagnóstico serológico y molecular del Potato leafroll virus (PLRV) en tubérculos-semilla de papa en Antioquia, Colombia

2015 ◽  
Vol 65 (2) ◽  
pp. 204-210 ◽  
Author(s):  
Mauricio Esteban Mesa Medina ◽  
Martha Isabel González Ramírez ◽  
Pablo Andrés Gutiérrez Sánchez ◽  
Mauricio Alejandro Marín Montoya
Keyword(s):  
Solanum Tuberosum ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Leafroll Virus ◽  
Rt Pcr ◽  
Potato Virus S ◽  
Leafroll Virus ◽  
Das Elisa

<p>Las virosis son uno de los problemas fitosanitarios más limitantes para la producción de papa, siendo los virus Potato leafroll virus (PLRV), Potato virus Y (PVY), Potato virus S (PVS) y Potato ye-llow vein virus (PYVV) los que generan mayores pérdidas económicas en la región andina. Gene-ralmente estos virus son transmitidos por insectos hemípteros y por tubérculos-semilla. En esta investigación se evaluó la presencia y distribución del PLRV en tubérculos de <em>Solanum tuberosum</em> variedad Diacol-Capiro y S. phureja variedad Criolla-Colombia obtenidos en Antioquia, utilizando pruebas serológicas y moleculares. Mediante DAS-ELISA se evaluaron 128 muestras consisten-tes en tres tipos de tejidos (piel, yemas latentes y brotes), mientras que con RT-PCR en tiempo real (RT-qPCR), se utilizó un subgrupo de 12 muestras de cada variedad. Utilizando DAS-ELISA, el PLRV se encontró en el 3,1% y 10,1% de las muestras de Diacol-Capiro y Criolla-Colombia, respectivamente; siendo evidente una mayor detección del virus en la piel de los tubérculos de ésta última variedad. Las pruebas de RT-qPCR detectaron el PLRV en 83,3% y 91,6% de las 12 muestras de Diacol-Capiro y de Criolla-Colombia, respectivamente; con valores de Ciclo umbral (CT) entre 17,72 y 30,59. La naturaleza de los amplicones obtenidos por RT-qPCR fue confirmada por secuenciación como parte de una región codificante de la cápside del PLRV (Identidad=99%-100%). Estos resultados enfatizan en la necesidad de utilizar tubérculo-semilla certificados por su sanidad viral en los cultivos de papa del país y de fortalecer dichos programas a partir del empleo de técnicas de detección altamente sensibles como RT-qPCR.</p>

scholarly journals 647 PB 413 REACTION OF POTATO CV. BELRUS TO INFECTION WITH POTATO LEAFROLL VIRUS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 525d-525
Author(s):  
Robert W. Goth ◽  
Ernest W. Goins ◽  
Kathleen G. Haynes
Keyword(s):  
Solanum Tuberosum ◽  
Virus Disease ◽  
Potato Leafroll Virus ◽  
Field Observations ◽  
Green Mountain ◽  
Aphid Infestation ◽  
Immunosorbant Assay ◽  
Enzyme Linked Immunosorbant Assay ◽  
Leafroll Virus ◽  
Bottom Portion

Potato leafroll virus (PLRV) is a serious aphid transmitted virus disease of potato (Solanum tuberosum L.). Field observations suggest that the cv. BelRus is tolerant to PLRV. Greenhouse grown BelRus and PLRV susceptible potato cvs. Green Mountain and Katahdin were tested for PLRV with enzyme linked immunosorbant assay (ELISA) and subsequently infested with PLRV infected green peach aphids (Myzus persicae). ELISA was used to test leaves from the top, middle and bottom portion of the plants at 7 day intervals beginning 7 days after aphid infestation. PLRV was detected in all tested locations of the Green Mountain and Katahdin plants 21 days after inoculation. In BelRus, throughout the 11 week test, PLRV was detected predominantly in the top portion of the plants and at low titres. These results suggest that tolerance to PLRV infection in the cv. BelRus may be due to suppression of virus replication.

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