Characterization of the Chromosomal Transmission of Intergeneric Hybrids of Saccharum spp. and Erianthus fulvus by Genomic in situ Hybridization

Crop Science ◽  
2010 ◽  
Vol 50 (5) ◽  
pp. 1642-1648 ◽  
Author(s):  
Xian-Hong Wang ◽  
Qing-Hui Yang ◽  
Fu-Sheng Li ◽  
Li-Lian He ◽  
Shun-Chang He
Keyword(s):  
In Situ Hybridization ◽  
Intergeneric Hybrids ◽  
Genomic In Situ Hybridization ◽  
Saccharum Spp

Characterization of wheat-Thinopyrum partial amphiploids by meiotic analysis and genomic in situ hybridization

Genome ◽  
2000 ◽  
Vol 43 (4) ◽  
pp. 712-719 ◽  
Author(s):  
George Fedak ◽  
Qin Chen ◽  
Robert L. Conner ◽  
André Laroche ◽  
René Petroski ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Genomic In Situ Hybridization ◽  
Meiotic Analysis ◽  
Partial Amphiploids

Meiotic chromosome pairing in intergeneric hybrids of colchicaceous ornamentals revealed by genomic in situ hybridization (GISH)

Euphytica ◽  
2014 ◽  
Vol 200 (2) ◽  
pp. 251-257 ◽  
Author(s):  
Tomonari Kishimoto ◽  
Miki Yamakawa ◽  
Daisuke Nakazawa ◽  
Junji Amano ◽  
Sachiko Kuwayama ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Chromosome Pairing ◽  
Meiotic Chromosome ◽  
Intergeneric Hybrids ◽  
Genomic In Situ Hybridization ◽  
Meiotic Chromosome Pairing

Cytological characterization of potato - Solanum etuberosum somatic hybrids and their backcross progenies by genomic in situ hybridization

Genome ◽  
1999 ◽  
Vol 42 (5) ◽  
pp. 987-992 ◽  
Author(s):  
F Dong ◽  
R G Novy ◽  
J P Helgeson ◽  
J Jiang
Keyword(s):  
In Situ Hybridization ◽  
Dna Marker ◽  
Somatic Hybrids ◽  
Molecular Cytogenetics ◽  
Genomic In Situ Hybridization ◽  
Preferential Pairing ◽  
Potato Germplasm ◽  
Solanum Etuberosum

Four somatic hybrids derived from a diploid wild species Solanum etuberosum and a diploid tuber-bearing Solanum clone 463-4, together with five BC1 and three BC2 plants, were analyzed by genomic in situ hybridization (GISH). None of the four somatic hybrids had the expected chromosome constitutions, i.e., 24 chromosomes from each fusion parent. Either one chromosome from S. etuberosum or one from the potato parent 463-4 was lost in the hybrids. Three BC1 plants had exactly one set of S. etuberosum chromosomes. The other two BC1 plants either had one extra or one fewer S. etuberosum chromosome, possibly because their somatic hybrid parents had an extra or had lost one S. etuberosum chromosome. The presence of one set, or close to one set, of S. etuberosum chromosomes in all BC1 plants suggests a preferential pairing and segregation of the S. etuberosum chromosomes in the somatic hybrids. Two of the three BC2 plants had 52 chromosomes, deviating significantly from the expected chromosome number of 48. These results suggest poor pairing between S. etuberosum and S. tuberosum chromosomes in the BC1 plants. The present study demonstrates the importance of combining GISH and DNA marker analysis for a thorough characterization of potato germplasm containing chromosomes from different species.Key words: potato germplasm, Solanum etuberosum, molecular cytogenetics.

Molecular cytogenetic analysis of wheat–barley hybrids using genomic in situ hybridization and barley microsatellite markers

Genome ◽  
2003 ◽  
Vol 46 (2) ◽  
pp. 314-322 ◽  
Author(s):  
L Malysheva ◽  
T Sjakste ◽  
F Matzk ◽  
M Röder ◽  
M Ganal
Keyword(s):  
Molecular Markers ◽  
In Situ Hybridization ◽  
Microsatellite Markers ◽  
Genetic Material ◽  
Introgressive Hybridization ◽  
Intergeneric Hybrids ◽  
Genomic In Situ Hybridization ◽  
Genome Constitution ◽  
Hybrid Plants

In the present investigation, genomic in situ hybridization (GISH) and barley microsatellite markers were used to analyse the genome constitution of wheat–barley hybrids from two backcross generations (BC1 and BC2). Two BC1 plants carried 3 and 6 barley chromosomes, respectively, according to GISH data. Additional chromosomal fragments were detected using microsatellites. Five BC2 plants possessed complete barley chromosomes or chromosome segments and six BC2 plants did not preserve barley genetic material. Molecular markers revealed segments of the barley genome with the size of one marker only, which probably resulted from recombination between wheat and barley chromosomes. The screening of backcrossed populations from intergeneric hybrids could be effectively conducted using both genomic in situ hybridization and molecular microsatellite markers. GISH images presented a general overview of the genome constitution of the hybrid plants, while microsatellite analysis revealed the genetic identity of the alien chromosomes and chromosomal segments introgressed. These methods were complementary and provided comprehensive information about the genomic constitution of the plants produced.Key words: wheat–barley hybrids, introgressive hybridization, recombination, molecular markers, genomic in situ hybridization (GISH).

Genomic in situ hybridization (GISH) analysis of intergeneric hybrids in Colchicaceae

Euphytica ◽  
2011 ◽  
Vol 181 (2) ◽  
pp. 197-202 ◽  
Author(s):  
Daisuke Nakazawa ◽  
Tomonari Kishimoto ◽  
Takeru Sato ◽  
Tomoka Saito ◽  
Junji Amano ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Intergeneric Hybrids ◽  
Genomic In Situ Hybridization ◽  
Gish Analysis
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