Interspecific crosses of onion with distant Allium species and characterization of the presumed hybrids by means of flow cytometry, karyotype analysis and genomic in situ hybridization

1996 ◽  
Vol 92 (3-4) ◽  
pp. 417-424 ◽  
Author(s):  
E. R. J. Keller ◽  
I. Schubert ◽  
J. Fuchs ◽  
A. Meister
Keyword(s):  
Flow Cytometry ◽  
In Situ Hybridization ◽  
Karyotype Analysis ◽  
Genomic In Situ Hybridization ◽  
Interspecific Crosses ◽  
Allium Species

Characterization of wheat-Thinopyrum partial amphiploids by meiotic analysis and genomic in situ hybridization

Genome ◽  
2000 ◽  
Vol 43 (4) ◽  
pp. 712-719 ◽  
Author(s):  
George Fedak ◽  
Qin Chen ◽  
Robert L. Conner ◽  
André Laroche ◽  
René Petroski ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Genomic In Situ Hybridization ◽  
Meiotic Analysis ◽  
Partial Amphiploids

Characterization by RFLP analysis and genomic in situ hybridization of a recombinant and a monosomic substitution plant derived from Hordeum vulgare L. × Hordeum bulbosum L. crosses

Genome ◽  
1997 ◽  
Vol 40 (2) ◽  
pp. 195-200 ◽  
Author(s):  
R. A. Pickering ◽  
A. M. Hill ◽  
R. G. Kynast
Keyword(s):  
In Situ Hybridization ◽  
Hordeum Vulgare ◽  
Rflp Analysis ◽  
Leaf Sheath ◽  
Genomic In Situ Hybridization ◽  
Barley Chromosome ◽  
Interspecific Crosses ◽  
Hordeum Bulbosum ◽  
Hordeum Vulgare L

Interspecific crosses in Hordeum have been made with the aim of transferring desirable traits, such as disease resistance, from a wild species, Hordeum bulbosum, into cultivated barley (Hordeum vulgare). Interspecific recombinants have previously been identified using several methods, but there are limitations with all the techniques. We improved our ability to characterize progeny from H. vulgare × H. bulbosum crosses by using genomic in situ hybridization (GISH). The plant material comprised a recombinant and a monosomic alien substitution plant derived from H. vulgare × H. bulbosum crosses. The recombinant possesses a pubescent leaf sheath conferred by a gene transferred from H. bulbosum into barley cultivar Golden Promise. The use of GISH on a plant homozygous for the pubescence gene confirmed the presence of H. bulbosum DNA located distally on two barley chromosomes and we mapped the introgression to barley chromosome 4HL using RFLP analysis. Furthermore, by means of an allelism test we found that the transferred gene for pubescence is allelic or closely linked to a gene for pubescence (Hs) located on barley chromosome 4HL. The presence of a single H. bulbosum chromosome in the monosomic substitution plant was confirmed by GISH. A distal introgression of H. bulbosum DNA was also observed on one barley chromosome, which was located on chromosome 3HL by RFLP analysis.Key words: Hordeum vulgare, Hordeum bulbosum, interspecific hybrid, gene introgression, genomic in situ hybridization.

Cytological characterization of potato - Solanum etuberosum somatic hybrids and their backcross progenies by genomic in situ hybridization

Genome ◽  
1999 ◽  
Vol 42 (5) ◽  
pp. 987-992 ◽  
Author(s):  
F Dong ◽  
R G Novy ◽  
J P Helgeson ◽  
J Jiang
Keyword(s):  
In Situ Hybridization ◽  
Dna Marker ◽  
Somatic Hybrids ◽  
Molecular Cytogenetics ◽  
Genomic In Situ Hybridization ◽  
Preferential Pairing ◽  
Potato Germplasm ◽  
Solanum Etuberosum

Four somatic hybrids derived from a diploid wild species Solanum etuberosum and a diploid tuber-bearing Solanum clone 463-4, together with five BC1 and three BC2 plants, were analyzed by genomic in situ hybridization (GISH). None of the four somatic hybrids had the expected chromosome constitutions, i.e., 24 chromosomes from each fusion parent. Either one chromosome from S. etuberosum or one from the potato parent 463-4 was lost in the hybrids. Three BC1 plants had exactly one set of S. etuberosum chromosomes. The other two BC1 plants either had one extra or one fewer S. etuberosum chromosome, possibly because their somatic hybrid parents had an extra or had lost one S. etuberosum chromosome. The presence of one set, or close to one set, of S. etuberosum chromosomes in all BC1 plants suggests a preferential pairing and segregation of the S. etuberosum chromosomes in the somatic hybrids. Two of the three BC2 plants had 52 chromosomes, deviating significantly from the expected chromosome number of 48. These results suggest poor pairing between S. etuberosum and S. tuberosum chromosomes in the BC1 plants. The present study demonstrates the importance of combining GISH and DNA marker analysis for a thorough characterization of potato germplasm containing chromosomes from different species.Key words: potato germplasm, Solanum etuberosum, molecular cytogenetics.

Paternity determination of interspecific rhododendron hybrids by genomic in situ hybridization (GISH)

Genome ◽  
2010 ◽  
Vol 53 (4) ◽  
pp. 277-284 ◽  
Author(s):  
Małgorzata Czernicka ◽  
Anna Mścichowska ◽  
Maria Klein ◽  
Piotr Muras ◽  
Ewa Grzebelus
Keyword(s):  
In Situ Hybridization ◽  
Mitotic Chromosome ◽  
Genomic In Situ Hybridization ◽  
Interspecific Crosses ◽  
Practical Tool ◽  
Paternity Determination ◽  
Hybrid Character ◽  
Positive Results

Genomic in situ hybridization (GISH) has been proved to be the most effective and accurate technique for confirmation of hybrid character. The objective of our study was to adapt and optimize a GISH protocol for identification of donor chromatin in hybrids obtained by interspecific crosses between five Rhododendron taxa ( R. aureum , R. brachycarpum , R. catawbiense ‘Catharine van Tol’, R. catawbiense ‘Nova Zembla’, and R. yakushimanum ‘Koichiro Wada’). Positive results were obtained only when we used mitotic chromosome spreads prepared from anthers. The best differentiation of maternal and paternal chromosomes in hybrid genomes was obtained when 50 ng of probe was applied together with blocking DNA at a concentration of 3.0 µg/µL. The results demonstrate that GISH is a practical tool for detection of alien genomes and analysis of the constitution of the chromosomes in rhododendron hybrids.

Characterization of the Chromosomal Transmission of Intergeneric Hybrids of Saccharum spp. and Erianthus fulvus by Genomic in situ Hybridization

Crop Science ◽  
2010 ◽  
Vol 50 (5) ◽  
pp. 1642-1648 ◽  
Author(s):  
Xian-Hong Wang ◽  
Qing-Hui Yang ◽  
Fu-Sheng Li ◽  
Li-Lian He ◽  
Shun-Chang He
Keyword(s):  
In Situ Hybridization ◽  
Intergeneric Hybrids ◽  
Genomic In Situ Hybridization ◽  
Saccharum Spp

Genome discrimination in progeny of interspecific hybrids between Brassica napus and Raphanus raphanistrum

Genome ◽  
2003 ◽  
Vol 46 (3) ◽  
pp. 469-472 ◽  
Author(s):  
A Benabdelmouna ◽  
G Guéritaine ◽  
M Abirached-Darmency ◽  
H Darmency
Keyword(s):  
In Situ Hybridization ◽  
Brassica Napus ◽  
Oilseed Rape ◽  
Interspecific Hybrid ◽  
Interspecific Hybrids ◽  
Genomic In Situ Hybridization ◽  
Interspecific Crosses ◽  
Wild Radish ◽  
Raphanus Raphanistrum

Genomic in situ hybridization (GISH) applied to the F1 interspecific hybrid between oilseed rape (Brassica napus, AACC, 2n = 38) and wild radish (Raphanus raphanistrum, RrRr, 2n = 18) showed the predicted 19 chromosomes from B. napus and 9 chromosomes from R. raphanistrum. The very low female fertility of these interspecific hybrids when backcrossed to R. raphanistrum led to only two descendants. Their chromosome number varied between 45 and 48. Both of these progenies showed only 9 chromosomes from R. raphanistrum and 36–39 chromosomes from B. napus. These results indicate the efficiency and limits of GISH as a suitable tool to assess and interpret the behavior of chromosomes after such interspecific crosses. The unexpected chromosome combination is discussed.Key words: genomic in situ hybridization, interspecific hybrid, introgression, oilseed rape, wild radish.

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