scholarly journals Glycolytic Inhibitor 2-Deoxy-D-Glucose Attenuates SARS-CoV-2 Multiplication in Host Cells and Weakens the Infective Potential of Progeny Virions

2021 ◽  
Author(s):  
Anant Narayan Bhatt ◽  
Abhishek Kumar ◽  
Yogesh Rai ◽  
Neeraj Kumari ◽  
Dhiviya Vedagiri ◽  
...  
Keyword(s):  
Host Cells ◽  
Glycolytic Inhibitor ◽  
Infective Potential

scholarly journals Glycolytic inhibitor 2-Deoxy-D-glucose attenuates SARS-CoV-2 multiplication in host cells and weakens the infective potential of progeny virions

2021 ◽  
Author(s):  
Anant Narayan Bhatt ◽  
Abhishek Kumar ◽  
Yogesh Rai ◽  
Neeraj Kumari ◽  
Dhiviya Vedagiri ◽  
...  
Keyword(s):  
Glucose Transporter ◽  
Acid Synthesis ◽  
Virus Multiplication ◽  
Metabolic Reprogramming ◽  
Host Cells ◽  
High Accumulation ◽  
Infected Cells ◽  
Glycolytic Inhibitor ◽  
Infective Potential

The COVID-19 pandemic is an ongoing public health emergency of international concern. Millions of people lost their lives to this pandemic. While a lot of efforts are being invested in vaccinating the population, there is also an emergent requirement to find potential therapeutics to effectively counter this fast mutating SARS-CoV-2 virus-induced pathogenicity. Virus-infected host cells switch their metabolism to a more glycolytic phenotype. This switch induced by the virus is needed for faster production of ATP and higher levels of glycolytic intermediates, which are required for anabolic processes such as fatty acid synthesis and nucleotide generation for new virion synthesis and packaging. In this study, we used 2-Deoxy-D-glucose (2-DG) to target and inhibit the metabolic reprogramming induced by SARS-CoV-2 infection. Our results showed that virus infection induces glucose influx and glycolysis resulting in selective high accumulation of the fluorescent glucose/2-DG analogue, 2-NBDG in these cells. Subsequently, 2-DG reduces the virus multiplication and alleviates the cells from infection-induced cytopathic effect (CPE) and cell death. Herein, we demonstrate that progeny virions produced from 2-DG treated cells are defective with compromised infectivity potential. Further, it was also observed that mannose inhibits 2-NBDG uptake at a very low concentration, suggesting that 2-DG uptake in virus-infected cells might be exploiting the specific mannose transporter or high-affinity glucose transporter, GLUT3, which was found to be increased on SARS-CoV-2 infection. In conclusion, our findings suggest that 2-DG effectively inhibits the SARS-CoV-2 multiplication and can be used as a treatment regimen. Based on these preliminary in-vitro findings this molecule reached clinical trial in COVID patients.

High Voltage Electron Microscopy of Viral Inclusion Bodies

1980 ◽  
Vol 38 ◽  
pp. 486-487 ◽  
Author(s):  
H.M. Mazzone ◽  
W.F. Engler ◽  
G. Wray ◽  
A. Szirmae ◽  
J. Conroy ◽  
...  
Keyword(s):  
New York ◽  
Environmental Protection Agency ◽  
Inclusion Bodies ◽  
Host Cells ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron ◽  
Viral Particles ◽  
Pest Insects ◽  
Viral Inclusion ◽  
Insect Gut

Viral inclusion bodies isolated from infected pest insects are being evaluated by the U.S. Dept. of Agriculture as biological insecticides against their hosts. Our research on these inclusion bodies constitutes part of an effort to support their approval by the Environmental Protection Agency as insect control agents. The inclusion bodies in this study are polyhedral in shape and contain rod-shaped viral particles. When ingested by pest insects, the inclusion bodies are broken down in the insect gut and release the viral particles which infect and multiply in the nuclei of host cells. These viruses are termed nucleopolyhedrosis viruses (NPV) and are representatives of the baculoviruses (Wildy, P. 1971 IN J.L. Melnick, ed., Monographs in Virology, vol. 5, S.Karger, New York).

Interaction between host cells and Salmonella Typhimurium isolates from septicemic pigs

2009 ◽  
Author(s):  
Nadia Bergeron ◽  
J. Corriveau ◽  
Ann Letellier ◽  
F. Daigle ◽  
L. Lessard ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Host Cells
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