scholarly journals Sensitive and precise method to measure the glycosidase activity with bicinchoninic acid

2003 ◽  
Vol 43 (supplement) ◽  
pp. S73
Author(s):  
S. Souma ◽  
Karim Nurul ◽  
H. Arikawa ◽  
S. Kidokoro
1968 ◽  
Vol 12 ◽  
Author(s):  
R. Goossens

A precise method for the determination of the increment of the  basal area using the PressIer bore. Refering to  previous research showing that the basal area of the corsica pine could be  characterized by an ellips, we present in this paper a precise method for the  determination of the increment of the basal area. In this method we determine  the direction of the maximum diameter, we measure this diameter and we take a  core in one of the points of tangency of the caliper with the measured tree.  The determination of the diameter perpendicular to the maximum diameter  finishes the work wich is to be done in the forest. From the classical  measurements effectuated on the core and from the measured diameters we can  then determine the form (V) and the excentricity (e). Substituting these two  parameters in the formula 2 or 2', we can also calculate the error of a  radius measured on the core with respect to the representative radius, This  error with them allow us to correct the measured value of the minimum or the  maximum radius and we will be able to do a precise determination of the  increment.


The Analyst ◽  
2006 ◽  
Vol 131 (8) ◽  
pp. 889 ◽  
Author(s):  
Tanin Tangkuaram ◽  
Jared Q. Gerlach ◽  
Yun Xiang ◽  
Abdel-Nasser Kawde ◽  
Zong Dai ◽  
...  

2021 ◽  
Vol 19 (7) ◽  
pp. 1508-1513
Author(s):  
Sengodagounder Muthusamy ◽  
Muthukumar Malarvizhi ◽  
Eringathodi Suresh

An unambiguous and precise method for the synthesis of 3,1-benzoxathiin-4-ones/1,3-benzodioxin-4-ones by the reaction of propargylic alcohols and salicylic/thiosalicylic acids under a catalyst-free and open-air atmosphere is described.


2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Gloria Gamiz-Arco ◽  
Luis I. Gutierrez-Rus ◽  
Valeria A. Risso ◽  
Beatriz Ibarra-Molero ◽  
Yosuke Hoshino ◽  
...  

AbstractGlycosidases are phylogenetically widely distributed enzymes that are crucial for the cleavage of glycosidic bonds. Here, we present the exceptional properties of a putative ancestor of bacterial and eukaryotic family-1 glycosidases. The ancestral protein shares the TIM-barrel fold with its modern descendants but displays large regions with greatly enhanced conformational flexibility. Yet, the barrel core remains comparatively rigid and the ancestral glycosidase activity is stable, with an optimum temperature within the experimental range for thermophilic family-1 glycosidases. None of the ∼5500 reported crystallographic structures of ∼1400 modern glycosidases show a bound porphyrin. Remarkably, the ancestral glycosidase binds heme tightly and stoichiometrically at a well-defined buried site. Heme binding rigidifies this TIM-barrel and allosterically enhances catalysis. Our work demonstrates the capability of ancestral protein reconstructions to reveal valuable but unexpected biomolecular features when sampling distant sequence space. The potential of the ancestral glycosidase as a scaffold for custom catalysis and biosensor engineering is discussed.


1983 ◽  
Vol 27 (9) ◽  
pp. 786-789 ◽  
Author(s):  
E.N. Corlett ◽  
J.A.E. Eklund

The backrest on a working seat reduces back muscle tension and the need to lock the spine for the transmission of horizontal forces from the trunk to the chair. These effects reduce inter-vertebral compression forces, which cause the discs to collapse. A simple and precise method for measuring shrinkage in stature has been developed and used to examine variations in the design of seats. The findings are discussed in relation to the biomechanics of sitting, and conclusions for seat design presented.


2014 ◽  
Vol 69 ◽  
pp. 62-71 ◽  
Author(s):  
Xuefeng Zhang ◽  
Bo Liu ◽  
Jieqiong Wang ◽  
Zhe Zhang ◽  
Kaibo Shi ◽  
...  

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