scholarly journals 2SC-01 Imaging of normal tailbud-stage Ciona intestinalis embryo at single cell level and Analysis of anatomy by constructing 3D Virtual Embryo(2SC Whole body imaging,The 49th Annual Meeting of the Biophysical Society of Japan)

2011 ◽  
Vol 51 (supplement) ◽  
pp. S15
Author(s):  
Kohji Hotta ◽  
Mitsuru Nakamura ◽  
Jun Terai ◽  
Reiko Ohkubo ◽  
Kotaro Oka
Keyword(s):  
Single Cell ◽  
Annual Meeting ◽  
Ciona Intestinalis ◽  
Whole Body ◽  
Whole Body Imaging ◽  
Single Cell Level ◽  
Cell Level ◽  
Body Imaging ◽  
Biophysical Society ◽  
Tailbud Stage

scholarly journals Dynamic Imaging of Marrow-Resident Granulocytes Interacting with Human Mesenchymal Stem Cells upon Systemic Lipopolysaccharide Challenge

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Jay T. Myers ◽  
Deborah S. Barkauskas ◽  
Alex Y. Huang
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Single Cell ◽  
Human Mesenchymal Stem Cells ◽  
Dynamic Imaging ◽  
Whole Body ◽  
Whole Body Imaging ◽  
Single Cell Level ◽  
Cell Level

Human mesenchymal stem cells (hMSCs) have gained intense research interest due to their immune-modulatory, tissue differentiating, and homing properties to sites of inflammation. Despite evidence demonstrating the biodistribution of infused hMSCs in target organs using static fluorescence imaging or whole-body imaging techniques, surprisingly little is known about how hMSCs behave dynamically within host tissues on a single-cell levelin vivo. Here, we infused fluorescently labeled clinical-grade hMSCs into immune-competent mice in which neutrophils and monocytes express a second fluorescent marker under the lysozyme M (LysM) promoter. Using intravital two-photon microscopy (TPM), we were able for the first time to capture dynamic interactions between hMSCs and LysM+granulocytes in the calvarium bone marrow of recipient mice during systemic LPS challenge in real time. Interestingly, many of the infused hMSCs remained intact despite repeated cellular contacts with host neutrophils. However, we were able to observe the destruction and subsequent phagocytosis of some hMSCs by surrounding granulocytes. Thus, our imaging platform provides opportunities to gain insight into the biology and therapeutic mechanisms of hMSCsin vivoat a single-cell level within live hosts.

scholarly journals 2SC-02 Coordination of mitosis and morphogenesis : Role of a prolonged G2 phase during chordate neural tube closure(2SC Whole body imaging,The 49th Annual Meeting of the Biophysical Society of Japan)

2011 ◽  
Vol 51 (supplement) ◽  
pp. S15-S16
Author(s):  
Yosuke Ogura ◽  
Asako Sakaue-Sawano ◽  
Masashi Nakagawa ◽  
Nori Satoh ◽  
Atsushi Miyawaki ◽  
...  
Keyword(s):  
Annual Meeting ◽  
Neural Tube ◽  
Neural Tube Closure ◽  
Whole Body ◽  
Whole Body Imaging ◽  
Body Imaging ◽  
Biophysical Society ◽  
G2 Phase ◽  
Tube Closure

scholarly journals 2SC-05 Imaging of structure, development and function of nervous system in a simple vertebrate(2SC Whole body imaging,The 49th Annual Meeting of the Biophysical Society of Japan)

2011 ◽  
Vol 51 (supplement) ◽  
pp. S16
Author(s):  
Kohei Hatta ◽  
Shinichi Okamoto ◽  
Masashi Nakagawa ◽  
Takanori Ikenaga ◽  
Tamami Yamamoto ◽  
...  
Keyword(s):  
Nervous System ◽  
Annual Meeting ◽  
Whole Body ◽  
Whole Body Imaging ◽  
Structure Development ◽  
Body Imaging ◽  
Biophysical Society ◽  
And Function

scholarly journals 2P-222 Measurement of conduction velocity in cardiomyocytes at the single-cell level for a cell network model(The 46th Annual Meeting of the Biophysical Society of Japan)

2008 ◽  
Vol 48 (supplement) ◽  
pp. S109
Author(s):  
Tomoyuki Kaneko ◽  
Fumimasa Nomura ◽  
Yuki Tomoe ◽  
Ikurou Suzuki ◽  
Junko Hayashi ◽  
...  
Keyword(s):  
Single Cell ◽  
Annual Meeting ◽  
Conduction Velocity ◽  
Network Model ◽  
Single Cell Level ◽  
Cell Level ◽  
Cell Network ◽  
Biophysical Society ◽  
A Cell
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