scholarly journals DNA-binding function of c-Myb R2R3 around thermal denaturation temperature

2021 ◽  
Vol 18 (0) ◽  
pp. 78-84
Author(s):  
Maki Kawasaki ◽  
Masayuki Oda
Keyword(s):  
Dna Binding ◽  
Thermal Denaturation ◽  
Denaturation Temperature ◽  
Binding Function ◽  
Thermal Denaturation Temperature

Mesophilic mutants of an obligate psychrophile, Micrococcus cryophilus

1969 ◽  
Vol 15 (10) ◽  
pp. 1145-1150 ◽  
Author(s):  
P-C. Tai ◽  
H. Jackson
Keyword(s):  
Growth Temperature ◽  
Metabolic Pathways ◽  
Thermal Denaturation ◽  
Deoxyribonucleic Acid ◽  
Elevated Temperatures ◽  
Maximum Growth ◽  
Maximal Growth ◽  
Denaturation Temperature ◽  
Maximum Growth Temperature ◽  
Thermal Denaturation Temperature

Several mutants with elevated maximal growth temperature (MGT) were developed from an obligate psychrophile, Micrococcus cryophilus ATCC 15174, by ultraviolet irradiation. Two of the mutants, T8 and M19, have the most similar characteristics to those of their parent. The mutants lost the ability to grow well at 0 °C and showed changes in metabolic pathways while acquiring the ability to grow at elevated temperatures. Heat resistance and deoxyribonucleic acid thermal denaturation temperature were shown to be unrelated to maximum growth temperature. The significance of the mutants is discussed.

Similar C-phycocyanins from two strains of thermotolerant cyanophyte Mastigocladus laminosus

1977 ◽  
Vol 23 (5) ◽  
pp. 510-517 ◽  
Author(s):  
Oranda H. W. Kao ◽  
Donald S. Berns
Keyword(s):  
Elevated Temperature ◽  
Thermal Denaturation ◽  
Ph Value ◽  
Blue Green Alga ◽  
Denaturation Temperature ◽  
Mastigocladus Laminosus ◽  
Thermal Denaturation Temperature ◽  
Temperature Ranges ◽  
Synechococcus Lividus ◽  
Immunologic Properties

C-phycocyanin from two strains of the thermotolerant blue-green alga, Mastigocladus laminosus (NZ-DB2-m and I-30-m), that grow within different temperature ranges have been characterized with respect to aggregation, immunologic properties, subunit composition, and thermodenaturation. The critical thermal-denaturation temperature for phycocyanin from both strains of M. laminosus phycocyanin is 60 °C which is higher than that for mesophilic phycocyanin. Immunodiffusion studies have shown that these two strains of M. laminosus exhibit no antigenic differences and are closely related to the mesophilic Plectonema calothricoides and the thermophilic Synechococcus lividus (strain 3). Neither phenol nor α-naphthol has any effect on phycocyanin aggregation in these two strains of M. laminosus. There is also no enhancement of formation of large aggregates at their elevated temperature of cultivation. Furthermore, the phycocyanin of both strains of M. laminosus does not demonstrate any large amount of 19S or higher aggregates at any pH value. These observations suggest that the mode of adaptation of M. laminosus phycocyanin to high temperature is different from that previously encountered. It is also important to note that phycocyanin is essentially unchanged whether it is extracted from the same strain, M. laminosus (NZ-DB2-m), grown at either 50° or 37 °C.

Effect of carboidiimide on thermal denaturation temperature of dentin collagen

2016 ◽  
Vol 32 (4) ◽  
pp. 492-498 ◽  
Author(s):  
Milena Cadenaro ◽  
Luca Fontanive ◽  
Chiara Ottavia Navarra ◽  
Pietro Gobbi ◽  
Annalisa Mazzoni ◽  
...  
Keyword(s):  
Thermal Denaturation ◽  
Denaturation Temperature ◽  
Thermal Denaturation Temperature ◽  
Dentin Collagen

Isolation and characterisation of acid- and pepsin-soluble collagen from the skin of Cervus korean TEMMINCK var. mantchuricus Swinhoe

2018 ◽  
Vol 58 (3) ◽  
pp. 585 ◽  
Author(s):  
Gaurav Lodhi ◽  
Yon-Suk Kim ◽  
Eun-Kyung Kim ◽  
Jin-Woo Hwang ◽  
Hyung-Sik Won ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Thermal Denaturation ◽  
Peptide Hydrolysis ◽  
Denaturation Temperature ◽  
Soluble Collagen ◽  
Acid Soluble Collagen ◽  
Thermal Denaturation Temperature ◽  
Skin Collagen ◽  
Pepsin Soluble Collagen ◽  
Calf Skin

Acid-soluble collagen and pepsin-soluble collagen were extracted from the skin of deer, Cervus korean TEMMINCK var. mantchuricus Swinhoe. The two types of collagen were then characterised using sodium dodecyl sulfate–polyacrylamide gel electrophoresis, amino acid composition analysis, peptide hydrolysis patterns, thermal denaturation temperature, differential scanning calorimetry, Fourier transform infrared spectroscopy, and nuclear magnetic resonance imaging. The yield of pepsin-soluble collagen (9.62%) was greater than that of acid-soluble collagen (2.24%), but both types of collagen showed similar electrophoretic patterns with each other and with calf skin collagen. The peptide hydrolysis pattern results suggested that calf skin collagen and pepsin-soluble collagen from deer skin may be similar in terms of their primary structure. The thermal denaturation temperature of acid-soluble collagen and pepsin-soluble collagen were 36.67°C and 36.44°C, respectively, and their melting temperatures were 110°C and 120°C, respectively, which suggest high thermal stability. Fourier transform infrared showed a triple helical structure and nuclear magnetic resonance confirmed the presence of ‘hydration’ water. These results provide a basis for large-scale production and further application as alternatives to other mammalian collagens.

scholarly journals Rif1 regulates telomere length through conserved HEAT repeats

2021 ◽  
Vol 49 (7) ◽  
pp. 3967-3980
Author(s):  
Calla B Shubin ◽  
Rini Mayangsari ◽  
Ariel D Swett ◽  
Carol W Greider
Keyword(s):  
Dna Binding ◽  
Telomere Length ◽  
Functional Role ◽  
Conserved Residues ◽  
Binding Interface ◽  
Binding Function ◽  
Length Regulation ◽  
Telomere Length Regulation ◽  
Telomere Regulation ◽  
Conserved Amino Acids

AbstractIn budding yeast, Rif1 negatively regulates telomere length, but the mechanism of this regulation has remained elusive. Previous work identified several functional domains of Rif1, but none of these has been shown to mediate telomere length. To define Rif1 domains responsible for telomere regulation, we localized truncations of Rif1 to a single specific telomere and measured telomere length of that telomere compared to bulk telomeres. We found that a domain in the N-terminus containing HEAT repeats, Rif1177–996, was sufficient for length regulation when tethered to the telomere. Charged residues in this region were previously proposed to mediate DNA binding. We found that mutation of these residues disrupted telomere length regulation even when Rif1 was tethered to the telomere. Mutation of other conserved residues in this region, which were not predicted to interact with DNA, also disrupted telomere length maintenance, while mutation of conserved residues distal to this region did not. Our data suggest that conserved amino acids in the region from 436 to 577 play a functional role in telomere length regulation, which is separate from their proposed DNA binding function. We propose that the Rif1 HEAT repeats region represents a protein-protein binding interface that mediates telomere length regulation.

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