scholarly journals Glycine insertion modulates the fluorescence properties of Aequorea victoria green fluorescent protein and its variants in their ambient environment

2021 ◽  
Author(s):  
Takamitsu J Morikawa ◽  
Masayoshi Nishiyama ◽  
Keiko Yoshizawa ◽  
Hideaki Fujita ◽  
Tomonobu M Watanabe
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Fluorescence Properties ◽  
Aequorea Victoria ◽  
Ambient Environment ◽  
Green Fluorescent

scholarly journals Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein

1997 ◽  
Vol 94 (6) ◽  
pp. 2306-2311 ◽  
Author(s):  
K. Brejc ◽  
T. K. Sixma ◽  
P. A. Kitts ◽  
S. R. Kain ◽  
R. Y. Tsien ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Structural Basis ◽  
Aequorea Victoria ◽  
Green Fluorescent

scholarly journals The First Mutant of the Aequorea victoria Green Fluorescent Protein That Forms a Red Chromophore†

Biochemistry ◽  
2008 ◽  
Vol 47 (16) ◽  
pp. 4666-4673 ◽  
Author(s):  
Alexander S. Mishin ◽  
Fedor V. Subach ◽  
Ilia V. Yampolsky ◽  
William King ◽  
Konstantin A. Lukyanov ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Aequorea Victoria ◽  
Green Fluorescent

Synthesis and fluorescence properties of 4-diarylmethylene analogues of the green fluorescent protein chromophore

Tetrahedron ◽  
2015 ◽  
Vol 71 (30) ◽  
pp. 4987-4998 ◽  
Author(s):  
Masahiro Ikejiri ◽  
Kousuke Matsumoto ◽  
Hiraku Hasegawa ◽  
Daisuke Yamaguchi ◽  
Moe Tsuchino ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Fluorescence Properties ◽  
Green Fluorescent

Shedding light on ultrafast ring-twisting pathways of halogenated GFP chromophores from the excited to ground state

2021 ◽  
Author(s):  
Sean Boulanger ◽  
Cheng Chen ◽  
Longteng Tang ◽  
Liangdong Zhu ◽  
Nadezhda S. Baleeva ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Ground State ◽  
Fluorescent Protein ◽  
Fluorescence Properties ◽  
Cellular Biology ◽  
Keen Interest ◽  
Green Fluorescent

Since green fluorescent protein (GFP) has revolutionized molecular and cellular biology for about three decades, there has been a keen interest in understanding, designing, and controlling the fluorescence properties of...

scholarly journals Influence of nanobody binding on fluorescence emission, mobility and organization of GFP-tagged proteins

2020 ◽  
Author(s):  
Falk Schneider ◽  
Christian Eggeling ◽  
Erdinc Sezgin
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescence Microscopy ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescence Emission ◽  
Fluorescence Properties ◽  
Great Care ◽  
Target Molecules ◽  
Green Fluorescent

SummaryAdvanced fluorescence microscopy studies require specific and monovalent molecular labelling with bright and photostable fluorophores. This necessity led to the widespread use of fluorescently labelled nanobodies against commonly employed fluorescent proteins. However, very little is known how these nanobodies influence their target molecules. Here, we observed clear changes of the fluorescence properties, mobility and organisation of green fluorescent protein (GFP) tagged proteins after labelling with an anti-GFP nanobody. Intriguingly, we did not observe any co-diffusion of fluorescently-labelled nanobodies with the GFP-labelled proteins. Our results suggest significant binding of the nanobodies to a non-emissive, oligomerized form of the fluorescent proteins, promoting disassembly into more monomeric forms after binding. Our findings show that great care must be taken when using nanobodies for studying dynamic and quantitative protein organisation.

scholarly journals Photoacid behaviour in a fluorinated green fluorescent protein chromophore: ultrafast formation of anion and zwitterion states

2016 ◽  
Vol 7 (9) ◽  
pp. 5747-5752 ◽  
Author(s):  
S. P. Laptenok ◽  
J. Conyard ◽  
P. C. Bulman Page ◽  
Y. Chan ◽  
M. You ◽  
...  
Keyword(s):  
Excited State ◽  
Green Fluorescent Protein ◽  
Proton Transfer ◽  
Transfer Reaction ◽  
Fluorescent Protein ◽  
Proton Transfer Reaction ◽  
Aequorea Victoria ◽  
Excited State Proton Transfer ◽  
Green Fluorescent

The photophysics of the chromophore of the green fluorescent protein in Aequorea victoria (avGFP) are dominated by an excited state proton transfer reaction.

Effects of Threonine 203 Replacements on Excited-State Dynamics and Fluorescence Properties of the Green Fluorescent Protein (GFP)

2000 ◽  
Vol 104 (19) ◽  
pp. 4791-4798 ◽  
Author(s):  
Andreas D. Kummer ◽  
Jens Wiehler ◽  
Hermann Rehaber ◽  
Christian Kompa ◽  
Boris Steipe ◽  
...  
Keyword(s):  
Excited State ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Fluorescence Properties ◽  
Excited State Dynamics ◽  
Green Fluorescent
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