scholarly journals Single-Step Method for the Determination of the Amount of the Common Deletion in Mitochondrial DNA

BioTechniques ◽  
1999 ◽  
Vol 26 (2) ◽  
pp. 290-300 ◽  
Author(s):  
F. Ahmed ◽  
E. Kougianos ◽  
J.M. Cummins ◽  
A.M. Jequier ◽  
J. Whelan
Keyword(s):  
Mitochondrial Dna ◽  
Single Step ◽  
Step Method ◽  
The Common ◽  
Common Deletion

Single step method for the accurate concentration determination of polysorbate 80

1997 ◽  
Vol 786 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Travis H. Tani ◽  
Jamie M. Moore ◽  
Thomas W. Patapoff
Keyword(s):  
Single Step ◽  
Polysorbate 80 ◽  
Step Method ◽  
Concentration Determination

scholarly journals Common Deletion (CD) in mitochondrial DNA of irradiated rat heart

2014 ◽  
Vol 86 (2) ◽  
pp. 685-694 ◽  
Author(s):  
RAQUEL G. SIQUEIRA ◽  
DAYSE A. DA SILVA ◽  
LUIZ D.B. DE MELO ◽  
ELIZEU F. DE CARVALHO ◽  
SAMARA C. FERREIRA-MACHADO ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Ionizing Radiation ◽  
Real Time ◽  
Real Time Pcr ◽  
Rat Heart ◽  
Wistar Rats ◽  
Post Irradiation ◽  
Male Wistar Rats ◽  
The Common ◽  
Common Deletion

The purpose of this study was to map the common deletion (CD) area in mtDNA and investigate the levels of this deletion in irradiated heart. The assays were developed in male Wistar rats that were irradiated with three different single doses (5, 10 or 15 Gy) delivered directly to the heart and the analyses were performed at various times post-irradiation (3, 15 or 120 days). The CDs area were sequenced and the CD quantified by real-time PCR. Our study demonstrated that the CD levels progressively decreased from the 3rduntil the 15th day after irradiation, and then increased thereafter. Additionally, it was observed that the levels of CD are modulated differently according to the different categories of doses (moderate and high). This study demonstrated an immediate response to ionizing radiation, measured by the presence of mutations in the CD area and a decrease in the CD levels.

Quantification of the common deletion in mitochondrial DNA of ICSI-Amniocytes and Non-ICSI-Amniocytes by competitive PCR

2003 ◽  
Vol 80 ◽  
pp. 39-40
Author(s):  
Sook H. Lee ◽  
Sung W. Cho ◽  
Hyun A. Kim ◽  
In P. Kwak ◽  
Jee E. Han ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Competitive Pcr ◽  
The Common ◽  
Common Deletion

Containerless Thermal Diffusivity Determination of High-Temperature Levitated Spherical Specimen by Extended Flash Methods: Theory and Experimental Validation

1997 ◽  
Vol 119 (2) ◽  
pp. 210-219 ◽  
Author(s):  
F. Shen ◽  
J. M. Khodadadi ◽  
M. C. Woods ◽  
J. K. R. Weber ◽  
B. Q. Li
Keyword(s):  
Heat Transfer ◽  
High Temperature ◽  
Thermal Diffusivity ◽  
Transfer Problem ◽  
Single Step ◽  
Temperature History ◽  
Step Method ◽  
Measured Time ◽  
Analytic Expressions

In order to determine the thermal diffusivity of materials, especially solids and liquids at high temperatures, two extended containerless flash techniques that are applicable to levitated spherical specimen are proposed. The extended flash methods are modeled as an axisymmetric transient conduction heat transfer problem within the sphere. For the “single-step” method, analytic expressions for the temperature history on the surface of the sphere are obtained that are independent of the incident energy and the absorption layer thickness. It is shown that by knowing the sample diameter and recording the temperature transient history at least at two different points on the surface simultaneously, the thermal diffusivity can be determined. A detailed discussion of the effects of the various parameters is presented. For the “two-step” analysis the problem of nonlinearity of the radiative heat transfer boundary condition is overcome by replacing it with the measured time-dependent surface temperature data. Upon obtaining the temperature field the determination of the thermal diffusivity turns into a minimization problem. In performing the proposed two-step procedure there is a need to undertake a cool-down experiment. Results of an experimental study directed at determining the thermal diffusivity of high-temperature solid samples of pure Nickel and Inconel 718 superalloy near their melting temperatures using the single-step method are discussed. Based on close agreement with reliable data available in the literature, it is concluded that the proposed techniques can provide reliable thermal diffusivity data for high-temperature materials.

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