scholarly journals Detection of hepatitis C virus core protein in serum by atomic force microscopy combined with mass spectrometry

2015 ◽  
pp. 1597 ◽  
Author(s):  
Ivan Shumov ◽  
Yuri Ivanov ◽  
Anna Kaysheva ◽  
Pavel Frantsuzov ◽  
Tatyana Pleshakova ◽  
...  
2018 ◽  
Vol 90 (7) ◽  
pp. 4596-4602 ◽  
Author(s):  
Wenhui Li ◽  
Xiaolong Kou ◽  
Jiachao Xu ◽  
Wei Zhou ◽  
Rong Zhao ◽  
...  

2014 ◽  
Vol 43 (1) ◽  
pp. 565-580 ◽  
Author(s):  
Ana García-Sacristán ◽  
Miguel Moreno ◽  
Ascensión Ariza-Mateos ◽  
Elena López-Camacho ◽  
Rosa M. Jáudenes ◽  
...  

2008 ◽  
Vol 80 (1) ◽  
pp. 237-245 ◽  
Author(s):  
Carla dos Santos Riccardi ◽  
Christine Kranz ◽  
Janusz Kowalik ◽  
Hideko Yamanaka ◽  
Boris Mizaikoff ◽  
...  

Micromachines ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 129 ◽  
Author(s):  
Tatyana Pleshakova ◽  
Anna Kaysheva ◽  
Ivan Shumov ◽  
Vadim Ziborov ◽  
Jana Bayzyanova ◽  
...  

In the present study, we demonstrate atomic force microscopy (AFM)-based detection of hepatitis C virus (HCV) particles in serum samples using a chip with aptamer-functionalized surface (apta-based AFM chip). The target particles, containing core antigen of HCV (HCVcoreAg protein), were biospecifically captured onto the chip surface from 1 mL of test solution containing 10 µL of serum collected from a hepatitis C patient. The registration of aptamer/antigen complexes on the chip surface was performed by AFM. The aptamers used in the present study were initially developed for therapeutic purposes; herein, these aptamers have been successfully utilized as probe molecules for HCVcoreAg detection in the presence of a complex protein matrix (human serum). The results obtained herein can be used for the development of detection systems that employ affine enrichment for protein detection.


1994 ◽  
Vol 68 (6) ◽  
pp. 3631-3641 ◽  
Author(s):  
E Santolini ◽  
G Migliaccio ◽  
N La Monica

Author(s):  
Rin Yamaguchi ◽  
Seiya Momosaki ◽  
Guang Gao ◽  
Chu Hsia ◽  
Masamichi Kojiro ◽  
...  

1995 ◽  
Vol 2 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Z. Chen ◽  
I. Berkower ◽  
R. Y.-H. Wang ◽  
W.-M. Ching ◽  
H. J. Alter ◽  
...  

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