DNA Fingerprinting by SSR-Analyses of Red Clover (Trifolium pratense L.) Cultivars

The results of experimental studies on polymorphism of 10 red clover cultivars of Russian selection are presented. Informative primer pairs that can be used for the identification of markers during intervariety differentiation were determined. Based on SSR fingerprints, molecular genetic formulas of the studied cultivars were compiled. Samples of genomic DNA of each cultivar were obtained from pooled aliquots of the plant tissue, each containing 30 seedlings. Eleven pairs of SSR primers developed for structural analysis of the red clover genome were used in genotyping. Unique amplification fragments were found with SSR locus RCS1307 for Trifon and Topaz cultivars, as well as with SSR locus RCS3095 for Trio and Mars cultivars, which can serve as identification DNA markers The results of this study are of practical importance for the molecular genetic certification of breeding achievements, which is necessary to assess the quality and genetic uniformity of seeds and to control hybridization in the breeding process. forage crops, red clover, DNA fingerprinting, SSR loci, cultivar identification This study was funded from the federal budget within the framework of a state assignment (project no. 0442-2019-0001 АААА-А19-119122590053-0).

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Efficiency of SSR and PawS markers for evaluation of genetic polymorphism among red clover (Trifolium pratense L.) cultivars

PROCEEDINGS ON APPLIED BOTANY GENETICS AND BREEDING ◽

10.30901/2227-8834-2020-3-100-109 ◽

2020 ◽

Vol 181 (3) ◽

pp. 100-109

Author(s):

I. A. Klimenko ◽

S. I. Kostenko ◽

Yu. M. Mavlyutov ◽

A. O. Shamustakimova

Keyword(s):

Dna Polymorphism ◽

Trifolium Pratense ◽

Red Clover ◽

Crop Varieties ◽

Pcr Products ◽

Ssr Loci ◽

Trifolium Pratense L ◽

The Mean ◽

Unique Amplicons ◽

Dna Profiles

Background. Identification of crop varieties is presently one of the most important aspects due a significant annual increase in the number of newly developed cultivars. Application of molecular markers makes it possible to identify cultivars and secure protection of plant breeders’ rights. Marker techniques based on SSR loci and PawS markers were evaluated for their efficiency in revealing the DNA polymorphism in Russian red clover cultivars, and the research results are presented in this publication.Materials and Methods. The total genome DNA was extracted by a modified SDS method from 30 seedlings per each cultivar. Nine simple sequence repeats (SSR) and 4 PawS markers were used for genotyping. The basic genetic diversity parameters were measured and analyzed using the software resources GelAnalyzer 2010а, MStools v.3, and Statistica 7.0.Results and conclusion. The mean level of intervarietal DNA polymorphism in red clover was 38.6%. Cultivar-specific amplicons were obtained for 4 accessions (cvs. ‘Trifon’, ‘Topaz’, ‘Trio’ and ‘Mars’) with SSR loci RCS1307 and RCS3095. These loci were found appropriate for identification and certification of such cultivars. The tested PawS markers (individually and in combinations) proved non-informative for the analysis of intervarietal DNA polymorphism in red clover. The only primer pair PawS5+PawS16 generated reproducible PCR products, but unique amplicons were absent in the DNA profiles. The data obtained in this study may be helpful for further identification and certification of Russian red clover cultivars and promising breeding materials.

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DNA fingerprinting of red clover (Trifolium pratense L.) with Jeffrey's probes: detection of somaclonal variation and other applications

Plant Cell Reports ◽

10.1007/bf00235293 ◽

1993 ◽

Vol 13 (2) ◽

pp. 72-78 ◽

Cited By ~ 4

Author(s):

Marek Nelke ◽

Jerzy Nowak ◽

Jonathan M. Wright ◽

Nancy L. McLean

Keyword(s):

Somaclonal Variation ◽

Dna Fingerprinting ◽

Trifolium Pratense ◽

Red Clover ◽

Trifolium Pratense L

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The biology of Canadian weeds. 121. Galium mollugo L.

Canadian Journal of Plant Science ◽

10.4141/p01-152 ◽

2003 ◽

Vol 83 (2) ◽

pp. 453-466 ◽

Cited By ~ 4

Author(s):

D. Mersereau ◽

A. DiTommaso

Keyword(s):

Invasive Species ◽

Trifolium Pratense ◽

Red Clover ◽

Phleum Pratense ◽

Forage Crops ◽

Weed Biology ◽

Melilotus Officinalis ◽

Orchard Grass ◽

Trifolium Pratense L ◽

Dactylis Glomerata L

Galium mollugo L. (smooth bedstraw) is a long-lived perennial broadleaved plant that reproduces both vegetatively and by seed. Galium mollugo can be a strong competitor in long-lived forage crops such as bird’s-foot trefoil (Lotus corniculatus L.), timothy (Phleum pratense L.), and orchard grass (Dactylis glomerata L.), as well as in short-lived forage crops such as red clover (Trifolium pratense L.) and yellow sweet-clover [Melilotus officinalis (L.) Lam.]. It is also a problem weed in spruce plantations and re-vegetation areas. Livestock typically avoid this species, allowing it to become well-established in pastures where it outcompetes more favourable species. Moreover, G. mollugo is viewed as a successful invasive species because of its ability to colonize and proliferate in areas such as established meadows where most invasive species do not thrive. In natural meadow communities, G. mollugo is often a dominant species that is capable of colonizing areas left vacant by the death and/or displacement of other species. Under favourable conditions, G. mollugo growth and clonal expansion can increase rapidly. Galium mollugo is generally tolerant to herbicides such as 2,4-D, MCPA, 2,4-DB, and dicamba. Key words: Smooth bedstraw, GALMO, Galium mollugo, Rubiaceae, weed biology, pastures

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Enetic diversity in red clover (Trifolium pratense L.) using SSR markers

Genetika ◽

10.2298/gensr1403949a ◽

2014 ◽

Vol 46 (3) ◽

pp. 949-961 ◽

Cited By ~ 5

Author(s):

Ramadan Ahsyee ◽

S Sanjavasiljevic ◽

Irena Calic ◽

Miroslav Zoric ◽

Djura Karagic ◽

...

Keyword(s):

Trifolium Pratense ◽

Molecular Genetic ◽

Red Clover ◽

Principal Coordinates Analysis ◽

Breeding Programs ◽

Average Value ◽

Molecular Genetic Diversity ◽

Principal Coordinates ◽

Trifolium Pratense L ◽

Efficient Breeding

Red clover (Trifolium pratense L.) is one of the main forage species from temperate regions and it's originated in Eurasia where wild-type populations are found in the Caucasus Mountains. Considerable variation for this crop has been recorded for few molecular variations. The identification and understanding of molecular genetic diversity in red clover accessions will help in effective genetic conservation along with efficient breeding programs in this crop. This study has shown molecular diversity using microsatellite markers in red clover accessions from around of the world. In this investigation, 40 genotypes have been selected and analyzed using 15 SSR primers. These primer pairs amplified 1146 polymorphic loci among the genotypes screened. The number of fragments amplified by each SSR primer combination varied from 24 for RCS3681 to 109 for RCS1729 with an average value of 80.78 per primer combination. The SSR marker data was further analyzed using cluster algorithms and Principal coordinates analysis (PCoA). The results indicated that the considerable genetic variations were discovered among the analyzed genotypes. The SSR based clustering could identify the putative pedigree types of the present red clover types of diverse origins.

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Interaction of the rate of P and K fertilization on the yield response of three forage legume species

Canadian Journal of Plant Science ◽

10.4141/cjps91-127 ◽

1991 ◽

Vol 71 (3) ◽

pp. 877-880

Author(s):

B. J. Zebarth ◽

R. W. Sheard

Keyword(s):

Response Surface ◽

Trifolium Pratense ◽

Red Clover ◽

Response Surface Model ◽

Yield Response ◽

Surface Model ◽

Soil Test ◽

Forage Crops ◽

Trifolium Pratense L ◽

K Fertilization

A response surface model was used to study the interaction between the rate of P and K fertilization on yield of three legume forage crops: alfalfa (Medicago sativa L.), trefoil (Lotus corniculatus L.), and red clover (Trifolium pratense L.). The results suggest that the soil test calibration recommendations developed for P or K on forage legumes are relatively insensitive to the rate of the other nutrient applied. Key words: Alfalfa, red clover, trefoil, soil test calibration, response surface model

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GENETIC CERTIFICATION OF FODDER CROPS BREEDING ACHIEVEMENTS

Vestnik of the Russian agricultural science ◽

10.30850/vrsn/2020/5/40-46 ◽

1970 ◽

pp. 40-46

Author(s):

V. M. Kosolapov ◽

N. N. Kozlov ◽

I. А. Klimenko ◽

V. N. Zolotarev

Keyword(s):

Dna Analysis ◽

Molecular Genetic ◽

Practical Importance ◽

Genetic Identification ◽

Necessary Condition ◽

Forage Crops ◽

Fodder Crops ◽

Long Time ◽

Analytical Review ◽

Prospects Of Application

The methods of genetic identification of forage crops varieties and forms have significant scientific and practical importance in breeding and seed multiplication, in protection of author’s rights. At the current moment molecular markers on the base of DNA-polymorphism have been applied widely for these aims. This analytical review examines the possibilities and the prospects of application the different DNA-analysis methods for assessment of forage crops genetic diversity and for development the molecular-genetic passports of breeding achievements. The objective estimation of varieties structure and presence impurities is a necessary condition for improving the methodical approaches in approbation of crops and for decision the problems of timely variety-seed renovation and its systematic replacement. The system of DNA markers that registered in genetic passport will enable to keep the initial genetic structure of variety and to maintain it in production process during long time without fluctuations of agronomic important characteristics and properties. This factor is especially valuable for development the primary seed multiplication.

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