Occurrence, Distribution, and Genetic Diversity of Alfalfa (Medicago sativa L.) Viruses in Four Major Alfalfa-Producing Provinces of China

Alfalfa (Medicago sativa L.) is one of the most widely cultivated forage crops in the world. China is the second largest producer of alfalfa in terms of the planting area worldwide, with Gansu, Henan, Inner Mongolia, and Shaanxi provinces being the production hubs. Alfalfa viruses have been reported on a small-scale survey in some of these areas, but they have not been well characterized. In the present study, seven viruses were detected in 12 fields of 10 cities/counties of the four abovementioned provinces by high-throughput sequencing and assembly of small RNA. Their incidence, distribution, and genetic diversity were analyzed by enzyme-linked immunosorbent assay, polymerase chain reaction (PCR)/reverse transcription-PCR and clone sequencing. The results showed that alfalfa mosaic virus (AMV), pea streak virus (PeSV), lucerne transient streak virus (LTSV), alfalfa dwarf virus (ADV), Medicago sativa alphapartitivirus 1 (MsAPV1), MsAPV2, and alfalfa leaf curl virus (ALCV) were the main viruses infecting alfalfa in four examined provinces. AMV and MsAPV1 had the highest incidences in all 4 provinces. SDT analysis of the 7 viruses isolated in China revealed a highly conserved among AMV, LTSV, ADV, MsAPV1, MsAPV2, and ALCV, but the sequence was a high variation between China isolates to abroad isolates in PeSV, ADV, and ALCV. To our knowledge, this is the first report of ADV in Inner Mongolia and Gansu, ALCV in Inner Mongolia, MsAPV1 and MsAPV2 in all 4 provinces, and PeSV and LTSV in China. These findings provide a basis for future research on the genetic evolution of alfalfa viruses in China and on strategies to prevent diseases in alfalfa caused by these viruses.

Productivity of fodder crops and their mixed planting depending on fertilizers and soil treatments

Relevance. The problem associated with the continuing decline in the yield of the main crops and soil fertility in most of the arable lands leads to the need of developing comprehensive measures, including the optimal selection of crops and methods of tillage and planting in order to increase the collection of plant protein and preserve soil fertility.Method. The work devoted to the study of the influence of tillage methods, mineral fertilizers and biological products on the productivity of forage crops and their mixed planting was carried out on the experimental fields of the Chechen Research Institute in 2019–2020 in the forest-steppe climatic zone. The experiment was multifactorial. Mineral fertilizers were used for crop rotation with a view to the planned yield. The biopreparation V417, created at the Institute of Agricultural Microbiology on the basis of the Bacillus subtilis strain, was used in the processing of seed material and crops during the period of intensive plant growth.Results. As a result of the research, it was revealed that each of the studied methods of tillage should correspond to the cultivated crop and the technology used. The introduction of mineral fertilizers provided an increase in the productivity of forage plants in their pure and mixed planting by 11–44%. The treatment of crops with biopreparation V417 reflected in the best way: an increase in yield was from 24 to 52%. At the same time, the highest yield of the green mass of 42.6 t/ha was obtained in a three-component mixture (wheat, peas, oats) in the variants where disking and chiseling were carried out. Mixed planting is a serious reserve for increasing the quantity and quality of plant products. It's feed productivity in the collection of dry matter and feed units was not inferior to pure planting of cereals, and in terms of the provision of 1 feed unit with digestible protein exceeded it by 3–14%.

Assessing the influence of cereal-legume mixtures on the productivity of degraded pastures in the Kostanay region of northern Kazakhstan

This study presents the results of some options to restore pastures with low productivity in the arid steppes of the Kostanay region of northern Kazakhstan, describing the effects associated with grass-legume mixtures. The effects of grass-legume mixtures, contribution to the preservation and maintenance of pasture forage crops, and the productivity of pastures were discussed. Mixtures of grasses and legumes were selected that are promising crops for arid regions.The plant density and its condition were determined based on test plots of adjacent rows of 0.5 m each, followed by counting. The plant height was determined before the yield of green mass by measuring 25 plants of each species. The yield of green mass in the maturity phase of the grass was determined by mowing and weighing the green mass in the plots, followed by the analysis of the species composition in the grass mixture and drying until air dry. The density of plants, the height of the plants, and the safety of the forage plants according to the sowing method were the data collected. In addition, the effect of grass mixtures on the productivity of forage crops to improve pastures was compared. According to these results, the highest productivity under experimental conditions was found in the wheat grassalfalfa-bromegrass variant. This information can contribute to the improvement of the state of the pastures since it is complete and inexpensive food for farm animals.

Terms of sowing forage crops for haylage production under the conditions of Central Yakutia

In recent years the agricultural production in the Republic of Sakha (Yakutia) has been introducing the technology of haylage production, which can replace part of the silage and hay in the ration of animals. However, stable raw materials of herbage are not enough for the technology of haylage production in Yakutia. Natural forage lands cannot provide a new technology of haylage production with herbage, since the entire area of haymaking goes to hay harvesting. Therefore, the selection of forage crops to create a reliable raw material base for haylage is a very relevant research topic. The purpose of the research was to study the terms of sowing forage crops for haylage production under the conditions in the Central Yakutia. The research has been carried out in the research and production permanent out-station in the M. G. Safronov Yakut Scientifi c Research Institute of Agriculture for three years. The experimental site was located on the second above-fl oodplain terrace of the Lena River Valley. The objects of research were the following varieties of zoned annual crops – oats of Pokrovsky variety 9, peas of Capital variety, spring vetch of Priobskaya variety 25, barley of Tammy variety. It has been found as a result of research that in the soil and climatic conditions in the Central Yakutia for the production of haylage for plant growth and development, dynamics of accumulation of dry matter, yield and nutritional qualities, the most effective mixtures are: vetch (1,2 million pcs/ha) + oats (2,5 million pcs/ha) and peas (0,8 million pcs/ha) + oats (2,5 million pcs/ha) germinating seeds. As the result of the conducted research, the optimal terms for sowing annual grasses for haylage have been established such as the 1st term (sowing in the third decade of May, harvesting in the first decade of August), the 2nd term (sowing in the second decade of June, harvesting in the third decade of August), the 3rd term (sowing in the first decade of July, harvesting in the first decade of September) in the phase of milk-wax ripeness in cereals and in the phase of fruit formation in legumes.

Ecological Intensification of Food Production by Integrating Forages

Forage crops have the potential to serve multiple functions, providing an ecological framework to sustainably intensify food production, i.e., ecological intensification. We review three categories of forages (annual forages, perennial forages, and dual-use perennial crops/forages) we believe hold the greatest promise for ecologically intensifying food production. Annual cover crops can provide additional forage resources while mitigating nutrient losses from agricultural fields when they are intercropped with, interseeded into, or following an annual crop, for instance. The integration of perennial forages either temporally, such as annual crop rotations that include a perennial forage phase, or spatially, such as the intercropping of perennial forages with an annual cash crop, provide weed suppression, soil quality, and yield and crop quality benefits. Dual-use crops/forages can provide forage and a grain crop in a single year while providing multiple ecological and economic benefits. However, tradeoffs in balancing multiple functions and limitations in reducing the risks associated with these practices exist. Advancing our understanding of these systems so we can overcome some of the limitations will play a critical role in increasing food production while promoting positive environmental outcomes.

Florida's Organo-Auxin Herbicide Rule—2021

Organo-auxin (phenoxy) herbicides have found a place in weed control schemes for peanut, corn, small grains, sugarcane, turf, pasture and forage crops, and many other areas. It is the intent of this publication to clarify and disseminate the Florida Organo-Auxin Herbicide Rule to interested growers and applicators. Major revision by B. Bultemeier, J. A. Ferrell, and G. E. MacDonald; 4 pages.https://edis.ifas.ufl.edu/wg051

Optimizing Yield and Quality of Silage and Hay for Pea–Barley Mixtures Ratio under Irrigated Arid Environments

The changing climate, water scarcity, reduction in the land area under forage crops, expanding ruminant population, burgeoning demands for milk and meat production and food security concerns all insist on a necessary increase in forage production and quality. Cereal–legume mixed intercropping offers a biologically superior option to boost herbage yield and quality of preserved fodder (hay and silage), provided that rationalization of the component crop’s proportional ratio is performed. Therefore, the current study was conducted to determine the forage yield and quality of barley (Hordeum vulgare L.) and pea (Pisum sativum L.) mixtures at the ratios 100:0, 75:25, 50:50, 25:75 and 0:100. The experiment was laid out in the regular arrangement of a randomized complete block design with three replicates. The results exhibited that forage quality was significantly affected by the mixture ratio. There was a significant improvement in protein, fiber rate and digestibility from the mixture. The ratios of barley and pea with 50:50 and 25:75 produced the maximum productivity and the best quality of forage. Regarding forage preservation, silage recorded higher contents of crude protein, ash and lower fiber contents than hay. Among seed mixtures, 0:100 ratio of barley–pea yielded the highest quality of silage with the maximum crude protein, ash and digestibility with lowest fiber contents.

Heavy Metals and Microbes Accumulation in Soil and Food Crops Irrigated with Wastewater and the Potential Human Health Risk: A Metadata Analysis

Wastewater is actively used for irrigation of vegetable and forage crops in arid lands due to water scarcity and cost advantages. The objective of this review was to assess the effect of wastewater (mixture sources) reuse in irrigation on soil, crop (vegetable and forage crops), animal products, and human health. The metadata analysis of 95 studies revealed that the mean of toxic heavy metals including nickel (Ni), chromium (Cr), cadmium (Cd), lead (Pb), and zinc (Zn) in untreated wastewater were higher than the world standard limits in wastewater-irrigated regions. Although heavy metals in treated wastewater were within the standard limits in those areas, the concentration of those toxic elements (Pb, Cd, Ni, Cr, and As) exceeded the allowable limits in both soil and vegetables’ edible parts. In fact, the concentration of heavy metals in vegetables’ edible parts increased by 3–9 fold when compared with those irrigated with fresh water. Escherichia coli in wastewater-irrigated soil was about 2 × 106 (CFU g−1) and about 15 (CFU g−1) in vegetables’ edible parts (leaf, bulb, tuber and fruit) while the mean total coliforms was about 1.4 × 106 and 55 (CFU g−1) in soil and vegetables’ edible parts, respectively. For human health risk assessment, the estimated daily intake (EDI) and human health risk index (HRI) ranged from 0.01 to 8 (EDI and HRI > 1.0 associated with adverse health effects). Although the mean of EDI for heavy metals from wastewater-irrigated vegetables were less than 1, the HRI for Cd and Pb were above the limits for safe consumption. Overall, heavy metal levels in wastewater that used for irrigation of agricultural crops could be within the recommended levels by the world standards, but the long-term use of this reused water will contaminate soil and crops with several toxic heavy metals leading to potential carcinogenic risks to humans. Therefore, rigorous and frequent testing (wastewater, soil, and plant) is required in cultivated farms to prevent the translocation of heavy metals in the food chain.

The phytosanitary risks posed by seeds for sowing trade networks

When successful, the operation of local and international networks of crop seed distribution or “seed systems” ensures farmer access to seed and impacts rural livelihoods and food security. Farmers are both consumers and producers in seed systems and benefit from access to global markets. However, phytosanitary measures and seed purity tests are also needed to maintain seed quality and prevent the spread of costly weeds, pests and diseases, in some countries regulatory controls have been in place since the 1800s. Nevertheless, seed contaminants are internationally implicated in between 7% and 37% of the invasive plant species and many of the agricultural pests and diseases. We assess biosecurity risk across international seed trade networks of forage crops using models of contaminant spread that integrate network connectivity and trade volume. To stochastically model hypothetical contaminants through global seed trade networks, realistic dispersal probabilities were estimated from quarantine weed seed detections and incursions from border security interception data in New Zealand. For our test case we use contaminants linked to the global trade of ryegrass and clover seed. Between 2014 and 2018 only four quarantine weed species (222 species and several genera are on the quarantine schedule) warranting risk mitigation were detected at the border. Quarantine weeds were rare considering that average import volumes were over 190 tonnes for ryegrass and clover, but 105 unregulated contaminant species were allowed in. Ryegrass and clover seed imports each led to one post-border weed incursion response over 20 years. Trade reports revealed complex global seed trade networks spanning >134 (ryegrass) and >110 (clover) countries. Simulations showed contaminants could disperse to as many as 50 (clover) or 80 (ryegrass) countries within 10 time-steps. Risk assessed via network models differed 18% (ryegrass) or 48% (clover) of the time compared to risk assessed on trade volumes. We conclude that biosecurity risk is driven by network position, the number of trading connections and trade volume. Risk mitigation measures could involve the use of more comprehensive lists of regulated species, comprehensive inspection protocols, or the addition of field surveillance at farms where seed is planted.

Forage production for dairy farming in terms of the Research Station “Poyma”

Важнейшей проблемой продовольственной безопасности страны является уровень обеспеченности населения молочными продуктами. В связи с этим большое значение имеет опыт работы передовых хозяйств такого профиля в определённых почвенно-климатических и природно-экологических условиях. ФГБУ «Опытная станция «Пойма» является одним из наиболее эффективных хозяйств в Московской области по производству молока. Общая площадь сельскохозяйственных угодий составляет 6135 га, из них 2000 га пашни. В структуре посевных площадей 95% и более занимают кормовые культуры, в том числе 12–14% — однолетние травы, 68–70% — многолетние травы и природные кормовые угодья, 15–16% — кукуруза на силос. Основное производство кормов сосредоточено на высокоплодородных пойменных землях, на долю которых приходится 84% кормовых угодий. В статье изложены результаты системного применения достижений науки и передового опыта по стабильному производству высококачественных объёмистых кормов. Проведён анализ состояния кормопроизводства и животноводства хозяйства с 1982 по 2020 год, освещены научные подходы к увеличению производства кормов и животноводческой продукции. Системное освоение передовых научных разработок по созданию высокопродуктивных кормовых посевов и стабильному производству высококачественных объёмистых кормов, их рациональному хранению и использованию, техническая модернизация позволили увеличить среднегодовой надой на корову с 4079 до 9708 кг, или более чем в 2,3 раза, при одновременном снижении расхода кормов на 1 кг молока с 1,37 до 0,90 корм. ед. при повышении рентабельности с 16 до 28%. Food safety of the country is highly affected by the availability of milk products. Therefore, the experience of leading farms is of great importance under certain environmental conditions. The Research Station “Poyma” is one of the leading milk producers in the Moscow region. Its fields occupy around 6135 ha. The proportion of forage crops amounts to 95% including 12–14% of annual grasses, 68–70% of perennial grasses and natural forage lands as well as 15–16% of maize for silage production. 84% of the total cultivation area are high-fertile floodlands. This article focuses on the application of the latest scientific findings and advanced experience for stable production of high-quality bulk fodder. The efficiency of forage production and Animal Husbandry was analyzed in the period from 1982 to 2020. The methods and practices were reviewed to optimize forage and animal product resources. Average annual milk yield was improved from 4079 to 9708 kg per cow, or by more than 2.3 times due to the introduction of the latest findings into the production process including the cultivation of high-productive forage crops, stabilization of bulk fodder supplies, feed effective storage and use. Forage consumption dropped from 1.37 to 0.90 feed units for the production of 1 kg of milk, payback increased from 16 to 28%.