scholarly journals Enetic diversity in red clover (Trifolium pratense L.) using SSR markers

Genetika ◽  
2014 ◽  
Vol 46 (3) ◽  
pp. 949-961 ◽  
Author(s):  
Ramadan Ahsyee ◽  
S Sanjavasiljevic ◽  
Irena Calic ◽  
Miroslav Zoric ◽  
Djura Karagic ◽  
...  
Keyword(s):  
Trifolium Pratense ◽  
Molecular Genetic ◽  
Red Clover ◽  
Principal Coordinates Analysis ◽  
Breeding Programs ◽  
Average Value ◽  
Molecular Genetic Diversity ◽  
Principal Coordinates ◽  
Trifolium Pratense L ◽  
Efficient Breeding

Red clover (Trifolium pratense L.) is one of the main forage species from temperate regions and it's originated in Eurasia where wild-type populations are found in the Caucasus Mountains. Considerable variation for this crop has been recorded for few molecular variations. The identification and understanding of molecular genetic diversity in red clover accessions will help in effective genetic conservation along with efficient breeding programs in this crop. This study has shown molecular diversity using microsatellite markers in red clover accessions from around of the world. In this investigation, 40 genotypes have been selected and analyzed using 15 SSR primers. These primer pairs amplified 1146 polymorphic loci among the genotypes screened. The number of fragments amplified by each SSR primer combination varied from 24 for RCS3681 to 109 for RCS1729 with an average value of 80.78 per primer combination. The SSR marker data was further analyzed using cluster algorithms and Principal coordinates analysis (PCoA). The results indicated that the considerable genetic variations were discovered among the analyzed genotypes. The SSR based clustering could identify the putative pedigree types of the present red clover types of diverse origins.

Detection and assesment of red clover genetic sources with self-compatibility for development the varieties with high and sustainable seed productivity

2017 ◽  
Author(s):  
М.Ю. Новосёлов ◽  
О.А. Старшинова ◽  
Л.В. Дробышева ◽  
Г.П. Зятчина
Keyword(s):  
Trifolium Pratense ◽  
Red Clover ◽  
Morphological Characters ◽  
Biological Properties ◽  
Limiting Factor ◽  
Seed Productivity ◽  
Vegetative Period ◽  
Average Value ◽  
Self Compatibility ◽  
Trifolium Pratense L

Основными лимитирующими факторами в развитии клеверосеяния являются дефицит семян и их высокая стоимость. Наши исследования были направлены на поиск, выделение и изучение самосовместимых генотипов клевера лугового (Trifolium pratense L.). Потомство образца № 3000 I1, генетического источника самосовместимости, обладало данным свойством на уровне от 18 до 100 %. Средняя величина завязываемости семян при самоопылении составила 77 %, что сопоставимо с результатом при перекрёстном опылении (93 %) и многократно превосходит показатель самосовместимости у диплоидных сортов (0,5–1 %). Все изученные формы обладали завязываемостью семян на уровне 46 % без триппинга цветков при механическом воздействии на соцветия. Цитологическое изучение пыльцы и анализирующие скрещивания с диплоидными формами показали, что данный образец имеет диплоидное состояние. Сравнение по основным морфо-биологическим признакам образца № 3000 I1 с сортами Ранний 2 и ВИК 7 показало, что он отличается более продолжительным вегетационным периодом, низкорослостью, формирует большее количество стеблей, отличается повышенной облиственностью, содержанием сухого вещества и протеина. Выявлена высокая наследуемость самосовместимости в реципрокных скрещиваниях с сортом Ранний 2. Уровень самосовместимости более 50 % в поколении F1 был достигнут у 53 % генотипов при прямых и 74 % — при обратных скрещиваниях. Таким образом, созданный исходный материал может использоваться в селекционной практике для получения гомозиготного линейного материала и создания многолинейных сортов клевера лугового с высокой и устойчивой семенной продуктивностью. The basic limiting factor in development of clover-sowing is the deficit and high cost of seeds. Our investigations were directed on search, selection and study of self-compatible genotypes of red clover (Trifolium pratense L.). Progeny of a genetic source with self-compatibility (№ 3000 I1) exhibited this property with the level from 18 to 100 %. The average value of seed setting was 77 % at self-pollination, which was comparable with this parameter at cross-pollination — 93 %, and exceeded in many times self-compatibility of diploid varieties — 0.5–1 %. Every investigated genotype set seeds by 46 % in the absence of tripping, by mechanical stimulation of inflorescence. Cytology study of pollen and analyzing crossings with diploid forms have shown that studied sample is diploid. The № 3000 I1 and the varieties “Ranniy 2” and “VIK 7” were compared on morphological characters and biological properties. The studied sample had longer vegetative period, abundant foliage, was stunting, formed more stems, and contained more dry matter and protein. Self-compatibility highly inherited at reciprocal crossing with diploid variety “Ranniy 2”. Among progeny F1, at the direct crossings we have selected 53 % of genotypes with 50 % rate of self-compatibility and at the backcrossing — 74 %. Thus, the studied initial material is recommended to use in red clover breeding for creation homozygous lines and multilinear varieties with high and sustainable seed productivity.  

scholarly journals Molecular and phenotypic diversity among chickpea (Cicer arietinum) genotypes as a function of drought tolerance

2018 ◽  
Vol 69 (2) ◽  
pp. 142 ◽  
Author(s):  
Supriya Sachdeva ◽  
C. Bharadwaj ◽  
Vinay Sharma ◽  
B. S. Patil ◽  
K. R. Soren ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Phenotypic Diversity ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Similarity Coefficients ◽  
Principal Coordinates Analysis ◽  
Breeding Programs ◽  
Average Value ◽  
Principal Coordinates

Diversity as a function of drought tolerance may be identified by morphological characters, and molecular tools used to find the most divergent genotypes for breeding programs for drought tolerance in future. The narrow genetic base of chickpea can be circumvented by using diverse lines in breeding programs. Forty chickpea genotypes were studied for their morphological and molecular diversity with an objective of identifying the most diverse drought-tolerant lines. In total, 90 alleles were detected with 3.6 alleles per locus. Polymorphism information content (PIC) values ranged from 0.155 to 0.782 with an average value of 0.4374 per locus. The size of amplified products ranged from 160 bp to 390 bp. Primer TA136 with eight alleles showed the highest PIC value of 0.7825, indicating its ability to differentiate the genotypes at molecular level. DARwin neighbour-joining tree analysis based on dissimilarity estimates was done for the molecular data and sequential agglomerative hierarchical non-overlapping (SAHN) grouping for the morphological data. It could clearly discriminate the tolerance and the sensitivity of genotypes. Two-dimensional principal coordinates analysis (PCoA) plot indicated good diversity for drought tolerance. The genetic similarity coefficients ranged from 0.115 (genotypes BGD72 to ICCV 5308) to 0.828 (genotypes ICCV 10316 to ICCV 92337).

Analysis of genetic diversity and structure in a genebank collection of red clover (Trifolium pratense L.) using SSR markers

2016 ◽  
Vol 15 (4) ◽  
pp. 376-379 ◽  
Author(s):  
Mamta Gupta ◽  
Vikas Sharma ◽  
Sunil K. Singh ◽  
Rakesh K. Chahota ◽  
Tilak R. Sharma
Keyword(s):  
Genetic Diversity ◽  
Trifolium Pratense ◽  
Fragment Size ◽  
Red Clover ◽  
Principal Component ◽  
Model Based Clustering ◽  
Average Value ◽  
Genetic Diversity And Structure ◽  
Trifolium Pratense L ◽  
High Level

AbstractGenetic diversity of a red clover global collection was assessed using 36 simple sequence repeat (SSR) primers representing all seven linkage groups (LGs). The number of fragments amplified ranged from 1 to 6 for all the primers. Primer RCS0060 detected highest number of fragments, whereas four SSRs viz., RCS0899, RCS1594, TPSSR40 and RCS6927 amplified single fragment. Size range of amplicons generated by all the primers varied from 100 to 400 bp. Polymorphism information content values ranged from 0.301 to 0.719 with an average value of 0.605. LG wise diversity analysis showed that LG 3 was most diverse (I = 0.65, Ht = 0.44), whereas LG-1 showed minimum diversity (I = 0.48, Ht = 0.26) for the microsatellites used. Bayesian model-based clustering inferred three genetically distinct populations in the red clover germplasm holding and showed considerable admixture in individuals within clusters. Neighbour-joining analysis showed intermixing of accessions within groups. Principal component analysis plot complemented the clustering shown by Structure and distinguished three populations to greater extent. Analysis of molecular variance showed that 91% of the genetic variation was residing within populations, while 9% variation was among populations. Overall, the results showed that a high level of genetic diversity is prevailing in this worldwide collection of red clover, which can be exploited for its genetic improvement through breeding approaches.

RESPONSE TO SELECTION FOR STEM LENGTH IN RED CLOVER EVALUATED AT WIDE AND NARROW SPACINGS

1984 ◽  
Vol 64 (4) ◽  
pp. 925-934 ◽  
Author(s):  
S. R. BOWLEY ◽  
N. L. TAYLOR ◽  
P. L. CORNELIUS ◽  
C. T. DOUGHERTY
Keyword(s):  
Recurrent Selection ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Stem Length ◽  
Yield Response ◽  
Response To Selection ◽  
Breeding Programs ◽  
Herbage Yield ◽  
Trifolium Pratense L ◽  
Selection For

Six cycles of phenotypic recurrent selection for stem length were conducted in a 91-cm-spaced planted population of red clover (Trifolium pratense L.) as a means of increasing herbage yield. The objectives of this investigation were (1) to compare the effectiveness of selection for stem length tested in transplants at wide (91 cm) and narrow (7 cm) spacings, and in row seedings and (2) to determine the effect of selection for stem length on herbage yield. Response to selection for stem length was obtained under all plantings; however, greater gains were observed at the wide transplant spacing (3.3 cm∙cycle−1) compared to the narrow transplant spacing (0.7 cm∙cycle−1) and row seeding (0.8 cm∙cycle−1). Densely spaced transplants gave performance similar to row seedings and may be of value to forage crop breeders in situations where yield or quality assessment is desired but seed supplies are insufficient for the establishment of seeded plots, such as in the early stages of breeding programs. Selection for stem length was effective in increasing herbage yield at first harvest but was associated with reduced persistance, decreased regrowth yields, and increased partitioning of dry matter towards the shoot rather than the root during seedling development. Selection for stem length in the regrowth or in the second year, rather than in the primary (seedling) growth, might have avoided the declines in persistence and regrowth yields.Key words: Trifolium pratense L., red clover, recurrent selection, stem length, density, herbage yield

DNA Fingerprinting by SSR-Analyses of Red Clover (Trifolium pratense L.) Cultivars

2020 ◽  
Vol 36 (6) ◽  
pp. 55-60
Author(s):  
V.A. Dushkin ◽  
V.P. Klimenko ◽  
A.O. Shamustakimova
Keyword(s):  
Dna Fingerprinting ◽  
Trifolium Pratense ◽  
Molecular Genetic ◽  
Practical Importance ◽  
Experimental Studies ◽  
Red Clover ◽  
Federal Budget ◽  
Forage Crops ◽  
Ssr Loci ◽  
Trifolium Pratense L

The results of experimental studies on polymorphism of 10 red clover cultivars of Russian selection are presented. Informative primer pairs that can be used for the identification of markers during intervariety differentiation were determined. Based on SSR fingerprints, molecular genetic formulas of the studied cultivars were compiled. Samples of genomic DNA of each cultivar were obtained from pooled aliquots of the plant tissue, each containing 30 seedlings. Eleven pairs of SSR primers developed for structural analysis of the red clover genome were used in genotyping. Unique amplification fragments were found with SSR locus RCS1307 for Trifon and Topaz cultivars, as well as with SSR locus RCS3095 for Trio and Mars cultivars, which can serve as identification DNA markers The results of this study are of practical importance for the molecular genetic certification of breeding achievements, which is necessary to assess the quality and genetic uniformity of seeds and to control hybridization in the breeding process. forage crops, red clover, DNA fingerprinting, SSR loci, cultivar identification This study was funded from the federal budget within the framework of a state assignment (project no. 0442-2019-0001 АААА-А19-119122590053-0).

Comparisons of Floral Response of Seed Lots of Dollard Red Clover, Trifolium pratense L. 1

Crop Science ◽  
1965 ◽  
Vol 5 (5) ◽  
pp. 425-428 ◽  
Author(s):  
R. J. Bula ◽  
R. G. May ◽  
C. S. Garrison ◽  
C. M. Rincker ◽  
J. G. Dean
Keyword(s):  
Trifolium Pratense ◽  
Red Clover ◽  
Trifolium Pratense L

scholarly journals Volatile Constituents of Trifolium Pratense and T. Repens from N.E. Italian Alpine Pastures

2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Aldo Tava ◽  
Daniele Ramella ◽  
Maris Grecchi ◽  
Paolo Aceto ◽  
Renato Paoletti ◽  
...  
Keyword(s):  
Steam Distillation ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Volatile Oil ◽  
Volatile Fraction ◽  
Fresh Weight ◽  
Fresh Weight Basis ◽  
Qualitative And Quantitative ◽  
Aerial Parts ◽  
Trifolium Pratense L

The composition of the volatile fraction of two important forage legumes from Italian sub-alpine N.E. pastureland, namely Trifolium pratense L. subsp. pratense (red clover) and T. repens subsp. repens (white clover) were investigated. The volatile oil was obtained from the fresh aerial parts by steam distillation and analyzed by GC/FID and GC/MS. The oil yield was 0.018 and 0.021% (weight/fresh weight basis) for T. pratense and T. repens, respectively. Several classes of compounds were found in both the oils, including alcohols, aldehydes, ketones, terpenes, esters, hydrocarbons, phenolics and acids. Qualitative and quantitative differences were found.

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