scholarly journals Diagnostic Performance of Line Probe Assay for the Diagnosis of Rifampicin and Isoniazid ‎Resistant Tuberculosis in a Resource-Poor Country ‎

Author(s):  
Laura Madukaji ◽  
Francis Ejeh ◽  
Eke Ofuche ◽  
Jay Samuel ◽  
Femi Owolagba ◽  
...  
2004 ◽  
Vol 18 (11) ◽  
pp. 658-664 ◽  
Author(s):  
Daniel W. Fitzgerald ◽  
Ascencio Maxi ◽  
Abdias Marcelin ◽  
Warren D. Johnson ◽  
Jean William Pape

MedEdPublish ◽  
2018 ◽  
Vol 7 (3) ◽  
Author(s):  
Ninos Oussi ◽  
Mitra Sadeghi ◽  
Javeria S. Qureshi ◽  
Charles Mabedi ◽  
Peter Elbe ◽  
...  

2019 ◽  
Vol 128 (9) ◽  
pp. 848-854 ◽  
Author(s):  
Vijayendra Honnurappa ◽  
Sangeetha Ramdass ◽  
Nilesh Mahajan ◽  
Vinay Kumar Vijayendra ◽  
Miriam Redleaf

Introduction: Necrotizing otitis externa resolves best with antimicrobial treatment. How to care for these patients and monitor their resolution remains a problem. Our protocol in Bangalore can manage these patients inexpensively and well. Materials and Methods: Patients who were referred to our patients became the subjects for this paper. They were managed through our protocol, which consists of IV ciprofloxacin and meropenem, weekly labs, weekly examinations, and photodocumention. Results: Fifty-one people presented with necrotizing otitis externa (NOE) between October 2015 and November 2017 and completed our entire protocol. Forty-six had complete resolution of their disease, while 5 had to undergo surgical removal of necrotic bone. Six of 8 patients with facial weakness had improvement in their House-Brackmann scores. Reduction of self-reported nocturnal pain, dissolution of ear canal granulations, and normalization of the erythrocyte sedimentation rate (ESR) proved to be the most accurate indicators of disease regression. Conclusion: Patients are monitored closely with review of their otalgia, examination of their canal, repeated ESRs, effective control of their diabetes, and radiological imaging. All this can be done in a resource-poor country, which in turn serves as a model for the wealthier nations.


2019 ◽  
Vol 45 (2) ◽  
Author(s):  
Angela Pires Brandao ◽  
Juliana Maira Watanabe Pinhata ◽  
Rosangela Siqueira Oliveira ◽  
Vera Maria Neder Galesi ◽  
Helio Hehl Caiaffa-Filho ◽  
...  

ABSTRACT Objective: To evaluate the rapid diagnosis of multidrug-resistant tuberculosis, by using a commercial line probe assay for rifampicin and isoniazid detection (LPA-plus), in the routine workflow of a tuberculosis reference laboratory. Methods: The LPA-plus was prospectively evaluated on 341 isolates concurrently submitted to the automated liquid drug susceptibility testing system. Results: Among 303 phenotypically valid results, none was genotypically rifampicin false-susceptible (13/13; 100% sensitivity). Two rifampicin-susceptible isolates harboured rpoB mutations (288/290; 99.3% specificity) which, however, were non-resistance-conferring mutations. LPA-plus missed three isoniazid-resistant isolates (23/26; 88.5% sensitivity) and detected all isoniazid-susceptible isolates (277/277; 100% specificity). Among the 38 (11%) invalid phenotypic results, LPA-plus identified 31 rifampicin- and isoniazid-susceptible isolates, one isoniazid-resistant and six as non-Mycobacterium tuberculosis complex. Conclusions: LPA-plus showed excellent agreement (≥91%) and accuracy (≥99%). Implementing LPA-plus in our setting can speed up the diagnosis of multidrug-resistant tuberculosis, yield a significantly higher number of valid results than phenotypic drug susceptibility testing and provide further information on the drug-resistance level.


2012 ◽  
Vol 16 (4) ◽  
pp. 532-538 ◽  
Author(s):  
Rotimi Williams Braimoh ◽  
Monica Omolara Mabayoje ◽  
Christiana Oluwatoyin Amira ◽  
Hubert Coker

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