Experimental study on resistance of air-water two-phase flow in a vertical 3 × 3 rod bundle was carried out under normal temperature and pressure. The rod diameter and pitch were 8 mm and 11 mm, respectively. The ranges of gas and liquid superficial velocity were 0.013∼3.763 m/s and 0.076∼1.792 m/s, respectively. The result indicated that the existing correlations for calculating frictional coefficient in the rod bundle and local resistance coefficient could not give favorable predictions on the single-phase experimental data. For the case of two-phase flow, eight correlations for calculating two-phase equivalent viscosity poorly predicted the frictional pressure drop, with the mean absolute errors around 60%. Meanwhile, the eight classical two-phase viscosity formulae were evaluated against the local pressure drop at spacer grid. It is shown that Dukler model predicted the experimental data well in the range of Rel<9000 while McAdams correlation was the best for Rel⩾9000. For all the experimental data, Dukler model provided the best prediction with MRE of 29.03%. Furthermore, approaches to calculate two-phase frictional pressure drop and local resistance were proposed by considering mass quality, two-phase Reynolds number and densities in homogenous flow model, resulting in a good agreement with the experimental data.