scholarly journals Molecular detection and phylogenetic analysis to evaluate the evolutionary pattern of VP1 gene sequence of recent duck hepatitis A virus ( DHAV) isolate and assessment of new genotyping emerging in Egypt.

2021 ◽  
Vol 41 (1) ◽  
pp. 29-33
Author(s):  
Ahmed El-Kholy ◽  
Ehab EL-Nahas ◽  
Mohammad Khodier ◽  
saad Sharawi
Keyword(s):  
Phylogenetic Analysis ◽  
Hepatitis A ◽  
Gene Sequence ◽  
Molecular Detection ◽  
Hepatitis A Virus ◽  
Vp1 Gene ◽  
Evolutionary Pattern ◽  
Duck Hepatitis

Molecular detection and typing of duck hepatitis A virus directly from clinical specimens

2008 ◽  
Vol 131 (3-4) ◽  
pp. 247-257 ◽  
Author(s):  
Yu Fu ◽  
Meng Pan ◽  
Xiaoyan Wang ◽  
Yongliang Xu ◽  
Hanchun Yang ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Molecular Detection ◽  
Hepatitis A Virus ◽  
Clinical Specimens ◽  
Duck Hepatitis

scholarly journals Genetic variation of the VP1 gene of the virulent duck hepatitis A virus type 1 (DHAV-1) isolates in Shandong province of China

2012 ◽  
Vol 27 (4) ◽  
pp. 248-253 ◽  
Author(s):  
Jiming Gao ◽  
Junhao Chen ◽  
Xingkui Si ◽  
Zhijing Xie ◽  
Yanli Zhu ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Virus Type ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Shandong Province ◽  
Vp1 Gene ◽  
Duck Hepatitis

scholarly journals A viroporin-like 2B protein of Duck Hepatitis A Virus 1that induces incomplete autophagy in DEF cells

2021 ◽  
pp. 101331
Author(s):  
Zezheng Liu ◽  
Qian Ye ◽  
Anchun. Cheng ◽  
Xuming. Ou ◽  
Sai Mao ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Duck Hepatitis ◽  
2B Protein

scholarly journals Development of a Real-Time Quantitative PCR for Detecting Duck Hepatitis A Virus Genotype C

2012 ◽  
Vol 50 (10) ◽  
pp. 3318-3323 ◽  
Author(s):  
Q. Huang ◽  
H. Yue ◽  
B. Zhang ◽  
P. Nie ◽  
C. Tang
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Genotype ◽  
Real Time Quantitative Pcr ◽  
Duck Hepatitis ◽  
Genotype C

Comparative pathogenicity of different subtypes of duck hepatitis A virus in Pekin ducklings

2019 ◽  
Vol 228 ◽  
pp. 181-187 ◽  
Author(s):  
Rongchang Liu ◽  
Shaohua Shi ◽  
Yu Huang ◽  
Zhen Chen ◽  
Cuiteng Chen ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Duck Hepatitis

scholarly journals Molecular epidemiology and genetic diversity of duck hepatitis A virus type 3 in Shandong province of China, 2012–2014

2017 ◽  
Vol 61 (04) ◽  
pp. 463-472 ◽  
Author(s):  
R. ZHANG ◽  
L. XIA ◽  
J. CHEN ◽  
Y. GONG ◽  
L. ZHANG ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Epidemiology ◽  
Virus Type ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Shandong Province ◽  
Duck Hepatitis ◽  
Type 3

scholarly journals Construction of Recombinant Lactococcus lactis Strain Expressing VP1 Fusion Protein of Duck Hepatitis A Virus Type 1 and Evaluation of Its Immune Effect

Vaccines ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 1479
Author(s):  
Xiaoting Zhang ◽  
Ruihua Zhang ◽  
Jingyu Wang ◽  
Nana Sui ◽  
Guige Xu ◽  
...  
Keyword(s):  
Immune System ◽  
Fusion Protein ◽  
Lactococcus Lactis ◽  
Virus Type ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Interleukin 2 ◽  
Interleukin 4 ◽  
Duck Hepatitis

With the continuous development of duck farming and the increasing breeding density, the incidence of duck hepatitis A virus type 1 (DHAV-1) has been on the rise, seriously endangering the development of duck farming. To reduce the use of antibiotics in duck breeding, susceptibility risks and mortality, and avoid virulence recovery and immune failure risk, this study aims to develop a new type of mucosal immune probiotics and make full use of molecular biology techniques, on the level of genetic engineering, to modify Lactococcus lactis (L. lactis). In this study, a secretory recombinant L. lactis named MG1363-VP1 with an enhanced Green Fluorescent Protein (eGFP) and translation enhancer T7g10L was constructed, which could express the VP1-eGFP fusion protein of DHAV-1. The animal experiment in ducklings was performed to detect the immune response and protection effect of oral microecologics by recombinant L. lactis. The results showed that oral L. lactis MG1363-VP1 significantly induced the body’s humoral immune system and mucosal immune system to produce specific anti-VP1 IgG antibodies and mucosal secretory immunoglobulin A (sIgA) for DHAV-1 in ducklings, and cytokines including interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-10 (IL-10), and interferon gamma (IFN-γ). The mortality rate was monitored simultaneously by the natural infestation in the process of production and breeding; notably, the ducklings vaccinated with L. lactis MG1363-VP1 were effectively protected against the nature infection of DHAV-1. The recombinant L. lactis MG1363-VP1 constructed in this study provides a new means of preventing and controlling DHAV-1 infection in the future.

scholarly journals Duck Hepatitis A Virus Type 1 Induces eIF2α Phosphorylation-Dependent Cellular Translation Shutoff via PERK/GCN2

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuanzhi Liu ◽  
Anchun Cheng ◽  
Mingshu Wang ◽  
Sai Mao ◽  
Xumin Ou ◽  
...  
Keyword(s):  
Virus Type ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Translation Efficiency ◽  
Eif2α Phosphorylation ◽  
Duck Hepatitis ◽  
Cellular Translation ◽  
First Time

Duck hepatitis A virus type 1 (DHAV-1) is one of the most deadly pathogens that endanger the duck industry. Most viruses usually turn off host translation after infection to facilitate viral replication and translation. For the first time report to our knowledge, DHAV-1 can induce eIF2α phosphorylation and inhibit cellular translation in duck embryo fibroblasts (DEFs). Moreover, the activity of DHAV-1 in the cells caused obvious eIF2α phosphorylation, which has nothing to do with the viral protein. Subsequently, we screened two kinases (PERK and GCN2) that affect eIF2α phosphorylation through inhibitors and shRNA. Notably, the role of GCN2 in other picornaviruses has not been reported. In addition, when the phosphorylation of eIF2α induced by DHAV-1 is inhibited, the translation efficiency of DEFs restores to a normal level, indicating that DHAV-1 induced cellular translation shutoff is dependent on eIF2α phosphorylation.

scholarly journals Virologic and Immunologic Characteristics in Mature Ducks with Acute Duck Hepatitis A Virus 1 Infection

2017 ◽  
Vol 8 ◽  
Author(s):  
Sai Mao ◽  
Mingshu Wang ◽  
Xumin Ou ◽  
Di Sun ◽  
Anchun Cheng ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Duck Hepatitis
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