scholarly journals Efficacy of triage parasite panel in diagnosis of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum antigens in symptomatic children stool specimens

2021 ◽  
Vol 17 (1) ◽  
pp. 84-91
Author(s):  
Gamal AboSheishaa
Keyword(s):  
Entamoeba Histolytica ◽  
Cryptosporidium Parvum ◽  
Giardia Lamblia ◽  
Stool Specimens

scholarly journals Detection of Giardia lamblia,Entamoeba histolytica/Entamoeba dispar, andCryptosporidium parvum Antigens in Human Fecal Specimens Using the Triage Parasite Panel Enzyme Immunoassay

2000 ◽  
Vol 38 (9) ◽  
pp. 3337-3340 ◽  
Author(s):  
Lynne S. Garcia ◽  
Robyn Y. Shimizu ◽  
Caroline N. Bernard
Keyword(s):  
Enzyme Immunoassay ◽  
Entamoeba Histolytica ◽  
Cryptosporidium Parvum ◽  
Giardia Lamblia ◽  
Gold Standard ◽  
Cross Reactivity ◽  
Entamoeba Dispar ◽  
Specific Antibodies ◽  
Fresh Frozen ◽  
Stool Specimens

The Triage parasite panel (BIOSITE Diagnostics, San Diego, Calif.) is a new qualitative enzyme immunoassay (EIA) panel for the detection of Giardia lamblia, Entamoeba histolytica/E. dispar, and Cryptosporidium parvum in fresh or fresh, frozen, unfixed human fecal specimens. By using specific antibodies, antigens specific for these organisms are captured and immobilized on a membrane. Panel performance was evaluated with known positive and negative stool specimens (a total of 444 specimens) that were tested by the standard ova and parasite (O&P) examination as the “gold standard,” including staining with both trichrome and modified acid-fast stains. Specimens with discrepant results between the reference and Triage methods were retested by a different method, either EIA or immunofluorescence. A number of samples with discrepant results with the Triage device were confirmed to be true positives. After resolution of discrepant results, the number of positive specimens and the sensitivity and specificity results were as follows: for G. lamblia, 170, 95.9%, and 97.4%, respectively; for E. histolytica/E. dispar, 99, 96.0%, and 99.1%, respectively; and for C. parvum, 60, 98.3%, and 99.7%, respectively. There was no cross-reactivity with other parasites found in stool specimens, including eight different protozoa (128 challenges) and three different helminths (83 challenges). The ability to perform the complete O&P examination should remain an option for those patients with negative parasite panel results but who are still symptomatic.

scholarly journals Simultaneous Detection of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum in Fecal Samples by Using Multiplex Real-Time PCR

2004 ◽  
Vol 42 (3) ◽  
pp. 1220-1223 ◽  
Author(s):  
J. J. Verweij ◽  
R. A. Blange ◽  
K. Templeton ◽  
J. Schinkel ◽  
E. A. T. Brienen ◽  
...  
Keyword(s):  
Real Time ◽  
Entamoeba Histolytica ◽  
Cryptosporidium Parvum ◽  
Real Time Pcr ◽  
Giardia Lamblia ◽  
Simultaneous Detection ◽  
Fecal Samples

scholarly journals Prevalence of Entamoeba histolytica and Giardia lamblia among schoolchildren in Um-Asher Area, Sudan

2020 ◽  
Author(s):  
Khalid Hajissa ◽  
Abd Elhafiz M.A. Muhajir ◽  
Tmador Abd All ◽  
M.S. Zakeia ◽  
Hamza Adam Eshag ◽  
...  
Keyword(s):  
Public Health ◽  
Entamoeba Histolytica ◽  
Giardia Lamblia ◽  
Primary Schools ◽  
Gastrointestinal Parasites ◽  
Health Concern ◽  
Health Issues ◽  
School Aged Children ◽  
Stool Specimens ◽  
Fresh Stool

Abstract Objective: Infections with Entamoeba histolytica and Giardia lamblia are widely prevalent and responsible for serious public health issues among school-aged children in developing countries. This study was conducted to determine the prevalence of these parasites among children from two primary schools in Um-Asher area, Khartoum, Sudan. A total 170 fresh stool specimens were collected from November 2017 to June 2018. The samples were examined by microscopy for the presence of the two parasites. Results: The overall prevalence of both parasites among the 170 children was 20%, with 13 children infected with E. histolytica (7.6%) and 23 with G. lamblia (13.5%). Most of the positive cases were single infections. Only two children (1.2%) had mixed infections. This indicates that these gastrointestinal parasites remain a challenging public health concern wherever sanitation and health measures are limited.

scholarly journals Comparative Pathobiology of the Intestinal Protozoan Parasites Giardia lamblia, Entamoeba histolytica, and Cryptosporidium parvum

Pathogens ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 116 ◽  
Author(s):  
Hemphill ◽  
Müller ◽  
Müller
Keyword(s):  
Entamoeba Histolytica ◽  
Cryptosporidium Parvum ◽  
Giardia Lamblia ◽  
Intestinal Tract ◽  
Protozoan Parasites ◽  
Intestinal Protozoan ◽  
Colon Wall ◽  
Causative Agents ◽  
Life Threatening ◽  
Human Intestinal Tract

Protozoan parasites can infect the human intestinal tract causing serious diseases. In the following article, we focused on the three most prominent intestinal protozoan pathogens, namely, Giardia lamblia, Entamoeba histolytica, and Cryptosporidium parvum. Both C. parvum and G. lamblia colonize the duodenum, jejunum, and ileum and are the most common causative agents of persistent diarrhea (i.e., cryptosporidiosis and giardiasis). Entamoeba histolytica colonizes the colon and, unlike the two former pathogens, may invade the colon wall and disseminate to other organs, mainly the liver, thereby causing life-threatening amebiasis. Here, we present condensed information concerning the pathobiology of these three diseases.

scholarly journals Prevalence of Cryptosporidium parvum/hominis, Entamoeba histolytica and Giardia lamblia among Young Children with and without Diarrhea in Dar es Salaam, Tanzania

2015 ◽  
Vol 9 (10) ◽  
pp. e0004125 ◽  
Author(s):  
Marit G. Tellevik ◽  
Sabrina J. Moyo ◽  
Bjørn Blomberg ◽  
Torunn Hjøllo ◽  
Samuel Y. Maselle ◽  
...  
Keyword(s):  
Young Children ◽  
Entamoeba Histolytica ◽  
Cryptosporidium Parvum ◽  
Giardia Lamblia ◽  
Dar Es Salaam
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